糖化低密度脂蛋白对成骨细胞低氧诱导因子1α表达的影响

发布时间：2018-05-26 19:15

本文选题：低密度脂蛋白 + 成骨细胞　；参考：《中国糖尿病杂志》2017年02期

【摘要】：目的研究不同糖化程度的低密度脂蛋白(LDL)对成骨细胞增殖及代谢的影响并探讨其可能机制。方法用不同程度(4.97%和8.24%)的糖化LDL(gly-LDL)处理小鼠成骨细胞(MC3T3-E1)48 h,设普通LDL(n-LDL)组和空白对照(Blank)组,测定缺氧诱导因子-1α(HIF-1α),VEGF mRNA表达,以及HIF-1α蛋白表达,同时检测细胞存活率及细胞上清骨钙素(OC)水平。结果 n-LDL组、4.97%gly-LDL组和8.24%gly-LDL组细胞存活率分别为95.6%、57.1%和71.2%。与Blank组比较,各实验组OC降低,且随着LDL的糖化,OC分泌受到抑制的程度越大。n-LDL组与Blank组比较,VEGFmRNA表达量下调(P0.05);8.24%gly-LDL组与n-LDL组比较,VEGF表达量相对上调。与Blank组及n-LDL组比较,8.24%gly-LDL上调HIF-1αmRNA的表达(P0.01),同时上调HIF-1α的蛋白表达。结论高糖化程度的LDL可影响小鼠成骨细胞HIF-1α的基因及蛋白表达影响,这可能是gly-LDL影响成骨细胞增殖与代谢的机制之一。
[Abstract]:Objective to study the effects of low density lipoprotein (LDL) on proliferation and metabolism of osteoblasts and its possible mechanism. Methods Murine osteoblasts MC3T3-E _ (1) were treated with glycosylated LDL (4.97% and 8.24%) for 48 h. The expression of mRNA and the expression of HIF-1 伪 were measured in normal LDL- (n-LDL) group and blank control group (Blank). Cell survival rate and osteocalcin (OC) level in supernatant were also measured. Results the cell survival rates in n-LDL group and 8.24%gly-LDL group were 57.1% and 71.2%, respectively. Compared with Blank group, OC of each experimental group decreased, and with the degree of inhibition of LDL secretion increased. N-LDL group and Blank group compared with Blank group, the expression of VEGF mRNA was down-regulated and the expression of VEGF mRNA was increased relatively compared with n-LDL group and P0.05%. 24gly-LDL group and n-LDL group. Compared with Blank group and n-LDL group, gly-LDL upregulated the expression of HIF-1 伪 mRNA and up-regulated the expression of HIF-1 伪 protein. Conclusion LDL with high degree of glycosylation can affect the expression of HIF-1 伪 gene and protein in osteoblasts, which may be one of the mechanisms of gly-LDL affecting the proliferation and metabolism of osteoblasts.
【作者单位】：同济大学附属同济医院内分泌科;上海中医药大学附属普陀医院内分泌科;
【基金】：863科研专项基金(2014AA022304) 上海市卫生局科研课题(20124256)
【分类号】：R587.1;R580

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