曲美他嗪降低1型糖尿病大鼠心肌组织炎症反应和氧化应激水平

发布时间：2018-05-26 21:52

本文选题：曲美他嗪 + 型糖尿病　；参考：《中山大学学报(医学科学版)》2016年03期

【摘要】：【目的】探讨曲美他嗪对链佐菌素诱导的1型糖尿病大鼠心肌组织肿瘤坏死因子-α(TNF-α)和超氧化物歧化酶-2(SOD-2)蛋白表达、心肌组织氧化应激水平及心脏功能的影响。【方法】选用6周龄雄性SD大鼠30只,随机分为3组:正常对照组(N组);糖尿病对照组(10只,C组);糖尿病联合曲美他嗪干预组(TMZ:15 mg·kg-1·d-1;N=10,TMZ组)。将链佐菌素溶解于0.1 mol/L柠檬酸缓冲液中,以40 mg·kg-1剂量腹腔注射大鼠,诱导1型糖尿病大鼠模型。TMZ组以曲美他嗪(15 mg·kg-1·d-1)灌胃,持续16周。16周后采用彩色多普勒超声及颈动脉导管检测大鼠心功能及血流动力学指标,Western blot技术及试剂盒检测TNF-α和SOD 2蛋白表达及丙二醛(MDA)水平,称量身体质量(BW)、大鼠左室质量(LVW)、全心质量(HW),HE染色观察心肌组织形态。【结果】与N组相比,C组心脏功能降低;LVW/HW及MDA增高(P0.05),而SOD-2表达降低(P0.001)。与C组比较,TMZ组TNF-α、MDA、LVEDP和LVW/HW降低,SOD 2升高(P0.05)。曲美他嗪治疗组心肌组织病理形态改变较糖尿病对照组减轻。【结论】心肌组织TNF-α表达上调和氧化应激水平升高在糖尿病发生发展中可能起重要作用。曲美他嗪可降低炎症因子TNF-α表达、改善氧化应激损伤、减缓心肌重塑和改善心脏舒张功能。
[Abstract]:[objective] to investigate the effects of trimetazidine on the expression of tumor necrosis factor- 伪 (TNF- 伪) and superoxide dismutase (SOD) -2- (SOD-2) protein in myocardial tissue of type 1 diabetic rats induced by streptozotocin. Effects of oxidative stress on myocardial tissue and cardiac function. [methods] Thirty male SD rats aged 6 weeks were selected. They were randomly divided into 3 groups: normal control group (n = 10), diabetic control group (n = 10), and diabetes combined with trimetazidine intervention group (n = 10). Streptozotocin was dissolved in 0. 1 mol/L citric acid buffer, and 40 mg kg-1 was injected intraperitoneally into rats. The model of type 1 diabetes was induced by intragastric administration of trimetazidine 15 mg kg-1 d-1. The expression of TNF- 伪 and SOD _ 2 protein and the level of malondialdehyde (MDA) were detected by using color Doppler ultrasound and carotid artery catheter after 16 weeks and 16 weeks later. The left ventricular mass (LVW), left ventricular mass (LVWN), total cardiac mass (TWH) and myocardial tissue morphology were observed by weighing BWN, left ventricular mass (LVW) and left ventricular mass (LVWN). [results] compared with group N, the cardiac function of group C decreased and LVW / HW and MDA increased (P 0.05), while the expression of SOD-2 decreased (P 0. 001). Compared with group C, TNF- 伪 MDA-LVEDP and LVW/HW decreased in TMZ group. [conclusion] the up-regulation of TNF- 伪 expression and the increase of oxidative stress may play an important role in the pathogenesis and development of diabetes mellitus. Trimetazidine could decrease the expression of TNF- 伪, improve oxidative stress injury, slow down myocardial remodeling and improve cardiac diastolic function.
【作者单位】：中山大学附属第三医院心内科;中山大学附属第三医院超声科;
【基金】：中国健康促进基金会心脏代谢治疗药物研究基金(20130507)
【分类号】：R587.2;R542.2

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