哮喘患者FCER2基因T2206C位点多态性与吸入糖皮质激素治疗反应的相关性

发布时间：2018-05-27 04:08

本文选题：哮喘 + FCER基因　；参考：《广东医学》2017年19期

【摘要】：目的探讨哮喘患者FCER2基因T2206C位点多态性与吸入糖皮质激素治疗反应的相关性。方法对153例哮喘患者采集外周静脉血测定嗜酸性粒细胞,并应用DNA提取、PCR扩增、基因测序观察FCER2基因T2206C位点多态性,且153例哮喘患者吸入布地奈德200μg、2次/d,治疗12周,并于治疗前后均进行ACT评分、肺功能测定。结果哮喘患者中T2206C位点存在基因多态现象,表型包括TT、TC和CC 3种基因型,分布频率分别为79例(51.6%)、49例(32.0%)、25例(16.3%)。哮喘患者T2206C位点基因型分布频率及等位基因频率比较均差异有统计学意义(P0.05)。治疗前在TT基因型组与CC基因型组哮喘患者中,ACT评分及EOS%比较差异有统计学意义(P0.05)。治疗后在TT基因型哮喘组,吸入布地奈德治疗前后第1秒用力呼气容积占预计值的百分比(FEV1/Pred%)、ACT评分比较均差异有统计学意义(P0.05)。结论哮喘患者存在着FCER2基因T2206C位点多态现象,其中TT基因型哮喘患者吸入糖皮质激素后肺功能、ACT评分改善情况均明显优于TC及CC基因型哮喘患者。FCER2基因T2206C位点多态性可能与哮喘患者ICS治疗反应存在一定相关性。
[Abstract]:Objective to investigate the association of T 2206 C polymorphism of FCER2 gene with corticosteroid inhalation in asthmatic patients. Methods Peripheral venous blood samples were collected from 153 asthmatic patients to determine eosinophils, and DNA extraction was used to amplify the eosinophils. The polymorphism of T2206C locus of FCER2 gene was observed by gene sequencing. In 153 asthmatic patients, budesonide 200 渭 g / d was inhaled for 12 weeks. ACT score and pulmonary function were measured before and after treatment. Results the polymorphism of T2206C locus was found in asthmatic patients. The phenotypes of T2206C locus included TTTC and CC genotypes. The distribution frequency of T2206C locus was 79 cases (51.66C) and 49 cases (32.0%) respectively. There were significant differences in genotype distribution and allele frequency of T2206C locus in asthmatic patients (P 0.05). There was significant difference in ACT score and EOS% between TT genotype group and CC genotype group before treatment. After treatment, in TT genotype asthma group, the percentage of forced expiratory volume to predicted value in 1 second before and after inhalation of budesonide was significantly higher than that of FEV 1 / Predna ACT score. Conclusion the polymorphism of T2206C locus of FCER2 gene exists in asthmatic patients. The improvement of pulmonary function score of TT genotype asthma patients after inhaling glucocorticoid was significantly better than that of TC and CC genotype asthma patients. The polymorphism of T2206C locus of FCER2 gene in patients with TT genotype asthma may be related to the ICS response of asthma patients.
【作者单位】：深圳市宝安区人民医院海华社康中心;深圳市宝安区人民医院呼吸科;
【基金】：深圳市宝安区医疗卫生科研项目(编号:2016CX184)
【分类号】：R562.25

【相似文献】