硫辛酸对糖尿病小鼠氧化还原状态及大血管并发症的影响

发布时间：2018-05-27 14:47

  本文选题：氧化应激 + 糖尿病　； 参考：《中国糖尿病杂志》2017年07期

【摘要】：目的建立糖尿病小鼠模型,研究硫辛酸(LA)干预后氧化还原状态、糖代谢酶活性和大血管的变化。方法小鼠分为正常对照(NC)组、糖尿病(DM)组和硫辛酸干预(LA)组,每组各10只,检测FBG、FIns,谷胱甘肽还原型氧化型比值(GSH/GSSG),超氧化物歧化酶活性抑制率(SODI),肝脏丙酮酸羧化酶(PC)和己糖激酶(HK)活性;HE染色观察胰岛细胞、电镜观察腹主动脉的微结构。结果 LA组干预6周后,FBG低于DM组[(10.05±0.40)vs(7.03±0.26)mmol/L,P0.05)];干预结束后GSH/GSSG、肝脏PC活性低于DM组[(23.15×10~(-2)±7.99×10~(-2))vs(36.20×10~(-2)±1.14×10~(-2)),(2.87×10~(-2)±0.32×10~(-2))vs(17.30×10~(-2)±4.01×10~(-2))Abs/μg pro/min,P0.05];胰岛细胞体积和排列、腹主动脉内皮细胞形态等较DM组恢复。结论 LA可以改善糖尿病小鼠模型氧化应激状态,减轻胰岛细胞和主动脉内皮的损伤。
[Abstract]:Objective to establish a diabetic mouse model and to study the redox state, the activity of glucose metabolism enzymes and the changes of large blood vessels after the intervention of lipoxylic acid (LAA). Methods the mice were divided into normal control group (n = 10), DM group (n = 10) and lipoic acid intervention group (n = 10). The activities of FBGN FIns, GSH / GSSGG, SOD activity inhibition rate (SODIN), hepatic pyruvate carboxylase (PCP) and hexokinase (HKK) were detected by HE staining, and the microstructures of abdominal aorta were observed by electron microscope. 缁撴灉 LA缁勫共棰，

本文编号：1942567