替诺福韦酯对小鼠成骨细胞MC3T3-E1增殖、凋亡及矿化的影响

发布时间：2018-05-28 06:07

本文选题：富马酸替诺福韦酯 + 成骨细胞　；参考：《中国骨质疏松杂志》2016年05期

【摘要】：目的探讨富马酸替诺福韦酯(Tenofovir disoproxil fumarate,TDF)对体外培养小鼠胚胎前体成骨样细胞MC3T3-E1增殖、凋亡及矿化的影响。方法体外培养MC3T3-E1细胞,分为正常细胞对照组、二甲基亚砜(dimethyl sulfoxide,DMSO)对照组、TDF处理组(50 nM、500 nM、5000 nM、50000 nM、250000 nM、500000 nM),干预24 h、48 h。cell counting kit-8(CCK8)试剂盒检测细胞增殖抑制率,ANNEXIN V/7AAD凋亡试剂盒检测细胞凋亡率,茜红素染色检测钙结节形成。结果TDF对MC3T3-E1成骨细胞的增殖和凋亡的影响受TDF浓度和干扰时间的影响,即低浓度对增殖和凋亡无影响,高浓度抑制增殖并促进凋亡,并随干扰时间增加,增殖抑制率和促凋亡率增大,并抑制MC3T3-E1成骨细胞钙结节形成;结论在血药浓度(约500 nM)时,TDF对MC3T3-E1成骨细胞增殖和凋亡无影响,但抑制钙结节形成,进而影响成骨,可能是导致TDF相关性骨量减少和骨质疏松的因素之一。
[Abstract]:Objective to investigate the effects of tenofovir disoproxil fumarate (TDF) on the proliferation, apoptosis and mineralization of mouse embryonic precursor osteoblast-like cells (MC3T3-E1) in vitro. Methods MC3T3-E1 cells were cultured in vitro and divided into normal cell control group and dimethyl sulfoxide-DMSO-treated group. The apoptosis rate was detected by ANNEXIN V/7AAD assay kit. The formation of calcium nodules was detected by hematoxylin staining. Results the effects of TDF on the proliferation and apoptosis of MC3T3-E1 osteoblasts were influenced by the concentration of TDF and the interfering time. Conclusion MC3T3-E1 has no effect on the proliferation and apoptosis of MC3T3-E1 osteoblasts at the concentration of 500nm, but inhibits the formation of calcium nodules and then affects the formation of osteoblasts. It may be one of the factors leading to TDF-related bone loss and osteoporosis.
【作者单位】：泰山医学院研究生部;首都医科大学附属北京地坛医院骨科;首都医科大学附属北京地坛医院传染病研究所新发突发传染病研究北京市重点实验室;潍坊医学院;
【分类号】：R580

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