生酮饮食导致小鼠骨质疏松的显微骨结构和生物力学研究

发布时间：2018-05-28 14:18

本文选题：生酮饮食 + 骨质疏松　；参考：《中国骨质疏松杂志》2017年03期

【摘要】：目的探讨酮体代谢小鼠骨结构和生物力学改变,以及所导致的小鼠骨质疏松。方法 10只8周龄的C57小鼠随机分为生酮饮食(KD)组和正常饮食(SD)组,每组5只,喂养3个月。生酮饮食的碳水化合物与脂肪的比例为1∶4。每两周监测小鼠体重、血糖和血酮。采用显微CT测量小鼠股骨远端松质骨的骨微结构和中段皮质骨的骨结构参数,厚度和面积。采用脱钙后的石蜡切片HE染色观察骨小梁分布。采用三点弯曲实验评价胫骨力学强度。结果 KD组血酮水平为0.8 mmol/L,明显高于SD组(0.2 mmol/L,P0.05)。KD组股骨远端松质骨的骨密度、骨体积分数和骨小梁连接密度分别为(49.5±3.6)mg HA/ccm、(4.1±0.5)%和(12.4±1.9)mm-3,而SD组分别为(69.1±4.0)mg HA/ccm、(6.4±1.0)%和(27.4±9.7)mm-3,两组间的差异均有统计学意义(P0.05)。两组间的骨小梁数目和厚度的差异没有统计学意义(P0.05)。KD组股骨中段皮质骨平均横截面积为(0.69±0.08)mm2,显著小于SD组(0.80±0.06)mm2。KD组胫骨三点弯曲的最大力、刚度和最大吸收能量分别为(7.3±0.7)N、(25.1±2.4)N/mm、(1.4±0.4)m J,显著小于SD组[(10.1±0.6)N、(33.4±2.9)N/mm、(2.3±0.5)m J]。结论本研究表明生酮饮食降低松质骨的骨密度、骨体积分数、骨小梁的连接程度,减少皮质骨的横截面积,降低骨力学强度,导致骨质疏松。
[Abstract]:Objective to investigate the changes of bone structure and biomechanics in mice with ketone body metabolism and the osteoporosis in mice. Methods Ten 8-week-old C57 mice were randomly divided into ketogenic diet (KD) group and normal diet (SDD) group with 5 mice in each group, fed for 3 months. The ratio of carbohydrates to fat in a ketogenic diet is 1: 4. Body weight, blood glucose and blood ketone were monitored every two weeks. Bone microstructure of cancellous bone of distal femur and bone structure parameters thickness and area of middle cortical bone were measured by microCT. The distribution of trabecular bone was observed by HE staining of decalcified paraffin sections. The mechanical strength of tibia was evaluated by three-point bending test. Results the plasma ketone level in KD group was significantly higher than that in SD group (0.2 mmol / L P0.05N 路KD group). The bone volume fraction and bone trabecular junction density were 49.5 卤3.6)mg 卤0.5 卤0.5% and 12.4 卤1.9 mm ~ (-3), respectively, and those in SD group were 69.1 卤4.0)mg 路ha ~ (-1) 卤1.0% and 27.4 卤9.7 m ~ (-3), respectively. The difference between the two groups was statistically significant (P 0.05). There was no significant difference in the number and thickness of bone trabeculae between the two groups. The average cross sectional area of the cortical bone in the middle segment of femur was 0.69 卤0.08 mm ~ 2 in P0.05N 路KD group, which was significantly lower than that in SD group (0.80 卤0.06)mm2.KD group). The stiffness and the maximum absorption energy of the SD group were (7.3 卤0.7) N ~ (-1) N ~ (-1) 卤2.4 ~ (-1) N 路m ~ (-1), respectively, which were significantly lower than those of the SD group [(10.1 卤0.6) N ~ (-1) N ~ (-1) N ~ (-1) 卤2.9N / m ~ (-1) 卤(2.3 卤0.5) m 路m ~ (-1) 路m ~ (-1). Conclusion the results showed that ketone diet reduced bone density, bone volume fraction, trabecular connection, cortical bone cross-sectional area and bone mechanical strength, resulting in osteoporosis.
【作者单位】：南方医科大学南方医院脊柱骨科;
【基金】：国家自然科学基金(81472084) 广东省自然科学基金(2015A030313276)
【分类号】：R580

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