糖尿病疼痛大鼠脊髓细胞自噬与凋亡相互作用的研究

发布时间：2018-05-29 14:57

本文选题：神经病理性疼痛 + 自噬　；参考：《实用医学杂志》2017年24期

【摘要】：目的探讨细胞自噬对糖尿病神经病理性疼痛(DNP)大鼠脊髓内细胞凋亡的影响。方法 SD大鼠腹腔注射链脲霉素(STZ)建立DNP模型,随机分为DNP组、DNP+雷帕霉素组(DNP-R组)、DNP+3-甲基嘌呤组(DNP-M组)及对照组(C组)。DNP-R及DNP-M组造模后鞘内注射雷帕霉素或3-甲基嘌呤(3-MA)。采用Western-Blot测定脊髓内LC3-II、Bcl-2、Caspase-3蛋白表达,采用TUNEL检测脊髓内细胞凋亡率。结果与DNP组相比,DNP-R组LC3-II表达显著上调,Caspase-3及Bcl-2蛋白表达显著下调,凋亡细胞比例显著减少,DNP-M组LC3-II表达显著下调,Caspase-3及Bcl-2蛋白表达显著上调,凋亡细胞比例显著增加。结论 DNP大鼠脊髓内自噬激活抑制细胞凋亡,反之自噬抑制促进细胞凋亡,表明两者间可能存在相互影响。
[Abstract]:Objective to investigate the effect of autophagy on cell apoptosis in the spinal cord of diabetic neuropathic pain (DNP) rats. Methods SD rats were intraperitoneally injected with streptozotocin (STZ) to establish DNP model and were randomly divided into DNP group, DNP+ rapamycin group (DNP-R group), DNP+3- methyl purine group (DNP-M group) and control group (C group).DNP-R and DNP-M group intrabinic injection thunder. Paramycin or 3- methyl purine (3-MA). The expression of LC3-II, Bcl-2, Caspase-3 protein in the spinal cord was measured by Western-Blot. The apoptosis rate of the spinal cord was detected by TUNEL. The expression of LC3-II in the spinal cord was significantly up-regulated in the DNP-R group. The expression of Caspase-3 and Bcl-2 protein was significantly down and the proportion of apoptotic cells decreased significantly. Down regulation, the expression of Caspase-3 and Bcl-2 protein was significantly up-regulated, and the proportion of apoptotic cells increased significantly. Conclusion autophagy activation inhibits apoptosis in the spinal cord of DNP rats, and the inhibition of autophagy promotes cell apoptosis, suggesting that there may be interaction between them.
【作者单位】：中山大学附属第三医院麻醉科;
【基金】：广东省自然科学基金-博士启动项目(编号:2016A030310157) 广东省医学科研基金项目(编号:A2015110)
【分类号】：R587.2

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