酸中毒致大鼠离体冠状动脉收缩的机制

发布时间：2018-05-29 18:54

本文选题：酸中毒 + 冠状动脉　；参考：《中国病理生理杂志》2017年05期

【摘要】：目的:探讨细胞外酸中毒(pH_(ex)6.8)致大鼠离体冠状动脉(RCA)收缩的机制。方法:采用离体微血管张力法,通过观察Na~+-H~+交换体1(NHE-1)选择性抑制剂cariporide(HOE-642)或Na~+-HCO_3~-共同转运体(NBC)抑制剂S0859预孵对pH_(ex)6.8所致RCA收缩的影响,探讨酸碱转运体在酸中毒收缩RCA中的作用;通过观察氯通道抑制剂NPPB和尼氟酸(NFA)预孵及细胞外去除氯离子对pH_(ex)6.8所致RCA收缩的影响,探讨氯离子通道在酸中毒收缩RCA中的作用。结果:pH_(ex)6.8引起RCA静息张力升高,最大张力为(3.90±0.95)mN。30μmol/L HOE-642和100μmol/L S0859均抑制pH_(ex)6.8引起的RCA收缩(P0.01)。NPPB和NFA均呈浓度依赖性地抑制pH_(ex)6.8引起的RCA收缩和KCl(60 mmol/L)引起的收缩。100μmol/L的NPPB和NFA均抑制U46619(1μmol/L)引起的RCA收缩(P0.01)。等摩尔NaBr代替细胞外液中NaCl后,几乎完全抑制pH_(ex)6.8引起的RCA收缩(P0.01),但是对KCl(60 mmol/L)或U46619(1μmol/L)引起的RCA收缩无显著影响。结论:细胞外液酸化所致的RCA收缩与激活NHE-1和NBC及促进氯离子跨细胞膜运动有关。
[Abstract]:Aim: to investigate the mechanism of extracellular acidosis induced by extracellular acidosis in isolated rat coronary artery (RCA). Methods: in vitro microvascular tension method was used to investigate the effects of selective inhibitor cariporide-HOE-642) or Na-HCO3-co-transporter (S0859) on RCA contraction induced by pH_(ex)6.8, and to explore the role of acid-base transporter in RCA induced by acidosis. The effect of chloride channel inhibitor NPPB and nifloric acid NFAs on RCA contraction induced by pH_(ex)6.8 was investigated by observing the effects of preincubation and extracellular chloride removal on RCA contraction induced by pH_(ex)6.8. Results the resting tension of RCA was increased in a dose-dependent manner. The maximum tension was 3.90 卤0.95)mN.30 渭 mol/L HOE-642 and 100 渭 mol/L S0859. Both of them inhibited RCA contraction induced by pH_(ex)6.8 in a concentration-dependent manner. NPPB and NFA inhibited pH_(ex)6.8 induced RCA contraction and KCl(60 mmol / L NPPB and NFA both inhibited RCA contraction induced by U46619 渭 mol / L (U46619n-1 渭 mol / L) in a concentration-dependent manner. When isomolar NaBr replaced NaCl in extracellular fluid, it almost completely inhibited RCA contraction induced by pH_(ex)6.8, but had no significant effect on RCA contraction induced by KCl(60 mmol / L or U46619 1 渭 mol / L. Conclusion: the contraction of RCA induced by extracellular fluid acidification is related to the activation of NHE-1 and NBC and the promotion of chloride ion transmembrane movement.
【作者单位】：山西医科大学药理学教研室;
【基金】：国家自然科学基金资助项目(No.81603111) 山西省生物优势学科基金山西医科大学博士启动基金(No.03201510)
【分类号】：R363

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