皮下和内脏脂肪细胞分布与大小和2型糖尿病的关系研究

发布时间：2018-05-30 17:48

本文选题：脂肪细胞 + 皮下脂肪　；参考：《浙江大学》2015年硕士论文

【摘要】：目的：肥胖是一个全球性的问题,中国人以腹型肥胖为主。肥胖和2型糖尿病密切相关,但是,肥胖如何能促进2型糖尿病的发生、发展仍未完全揭晓。但是很多文献指出,腹部脂肪的分布及脂肪细胞的大小与胰岛素抵抗密切相关。本研究旨在通过比较腹部皮下脂肪、内脏脂肪(网膜、肠系膜)分布及细胞大小与2型糖尿病、代谢状态的关系。方法：选取30名进行减肥手术的过度肥胖的患者,分为肥胖合并2型糖尿病组和单纯肥胖组,记录入院临床资料。用胶原酶消化法分离取自腹部皮下脂肪组织(Sc)细胞、网膜脂肪(Om)细胞、肠系膜脂肪(Mes)细胞。每个部位用电子显微镜分别截取350个脂肪细胞以测量直径。结果：1.总体来说,Sc脂肪细胞要大于Om脂肪细胞和Mes脂肪细胞(98.5μm vs88.8gm,p=0.100和86.7μm,p=0.036)。2.对于Sc脂肪细胞和Om脂肪细胞,肥胖合并2型糖尿病的患者,小细胞比例明显增加(14.9% vs 31.4%,p=0.006和14.0% vs 30.5%,p=0.015),应地,大细胞比例明显减少。对于Mes脂肪细胞,肥胖合并2型糖尿病的患者也有相同的趋势,但是没有达到统计学差异(p=0.090)。对于0m和Mes脂肪细胞,肥胖合并2型糖尿病患者中小细胞的直径也明显减小。3.空腹血糖和0m脂肪细胞和Mes脂肪细胞小细胞的比例成正相关(r=0.409,p=0.028 and r=0.523,p=0.004)。HBA1C和Om脂肪细胞小细胞的比例成正比(r=0.423,p=0.028)。Sc、Om、Mes脂肪细胞中大细胞的直径未与血糖、血脂、BMI等临床参数相关。结论：腹部Sc、Om、Mes脂肪细胞中小细胞比例的增加和2型糖尿病高度相关。
[Abstract]:Objective: obesity is a global problem in China. Obesity is closely related to type 2 diabetes. However, how obesity can promote the development of type 2 diabetes is not fully understood. However, many literatures indicate that the distribution of abdominal fat and the size of adipocytes are closely related to insulin resistance. The purpose of this study was to compare the distribution of abdominal subcutaneous fat, visceral fat (omentum, mesentery) and cell size with type 2 diabetes mellitus. Methods: 30 obese patients were divided into two groups: obesity combined with type 2 diabetes mellitus group and simple obesity group. The clinical data of admission were recorded. The cells were isolated from abdominal subcutaneous adipose tissue, omentum adipose tissue and mesenteric fat Mesa cells by collagenase digestion. The diameter of 350 adipocytes was measured by electron microscope. The result is 1: 1. On the whole, Sc adipocytes were larger than Om adipocytes and Mes adipocytes (98.5 渭 m vs 88.8gm-1, 0.100 and 86.7 渭 m). For SC adipocytes and Om adipocytes, the proportion of small cells in obese patients with type 2 diabetes increased significantly by 14.9% vs 31.4p0. 006 and 14.0% vs 30.5%. For Mes adipocytes, the same trend was found in obese patients with type 2 diabetes, but there was no statistical difference between them. For 0 m and Mes adipocytes, the diameters of small cells in obese patients with type 2 diabetes also decreased significantly. There was a positive correlation between fasting blood glucose and the ratio of 0 m adipocytes to small cells of Mes adipocytes. There was no correlation between the diameter of large cells in adipocytes of 0 m and 0. 409 and / 0. 028 and / L 0. 0023 and. HBA1C and Om adipocytes. The diameter of large cells in adipocytes was not correlated with the clinical parameters such as blood glucose, serum lipids and serum lipids. Conclusion: there is a high correlation between the increase of small cell proportion and type 2 diabetes mellitus.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.1;R589.2

【共引文献】