血管活性肠肽对实验性自身免疫性脑脊髓炎大鼠脑组织IFN-γ、IL-4因子含量的影响

发布时间：2018-06-02 17:59

本文选题：血管活性肠肽 + 实验性自身免疫性脑脊髓炎　；参考：《东南大学学报(医学版)》2017年01期

【摘要】：目的:探讨血管活性肠肽(VIP)对实验性自身免疫性脑脊髓炎(EAE)大鼠脑组织IFN-γ、IL-4含量的影响。方法:将60只健康雌性Wistar大鼠随机分成正常对照组,EAE对照组,VIP低剂量、高剂量干预组。利用髓鞘碱性蛋白(MBP)+完全福氏佐剂(CFA)建立EAE模型。自造模当日起,每隔1 d分别对VIP低、高剂量干预组大鼠腹腔注射VIP 4 nmol·kg-1(0.2 ml)、16 nmol·kg-1(0.8 ml),正常对照组及EAE对照组则给予生理盐水0.8 ml,连续10 d。观察大鼠发病情况、脑组织中IFN-γ、IL-4因子含量及脑组织HE染色。结果:与EAE对照组比较,VIP各剂量干预组大鼠发病潜伏期延长、进展期时间缩短、发病高峰期神经功能障碍评分(NDS)降低;脑组织中IFN-γ含量降低、IL-4含量升高,且VIP高剂量干预组效果最明显;脑组织HE染色显示炎症细胞浸润程度下降。结论:VIP通过降低脑组织中IFN-γ、升高IL-4含量减轻脑组织炎症细胞浸润,发挥对EAE的免疫调节作用。
[Abstract]:Aim: to investigate the effect of vasoactive intestinal peptide (VIP) on the content of IFN- 纬 -IL-4 in the brain of rats with experimental autoimmune encephalomyelitis (EAE). Methods: sixty healthy female Wistar rats were randomly divided into two groups: normal control group, low dose group and high dose group. The EAE model was established by using the complete Freund's adjuvant CFAs of myelin basic protein (MBP). From the same day, the rats in the low VIP group and the high dose group were injected intraperitoneally with VIP 4 nmol kg-1(0.2 Mel 16 nmol kg-1(0.8 ml, while the normal control group and EAE control group were given normal saline 0. 8 ml for 10 days. The incidence, the level of IFN- 纬 -IL-4 in brain tissue and HE staining in brain tissue were observed. Results: compared with the control group, the incubation period was prolonged, the time of progression was shortened, the neurologic dysfunction score was decreased, the content of IFN- 纬 in brain tissue was decreased, and the content of IL-4 was increased in the brain tissue. The effect of high dose VIP intervention group was the most obvious, and HE staining of brain tissue showed that the degree of inflammatory cell infiltration decreased. Conclusion by decreasing IFN- 纬 in brain tissue, increasing the content of IL-4 can reduce the infiltration of inflammatory cells in brain tissue and play an important role in the immune regulation of EAE.
【作者单位】：西南医科大学附属医院神经内科;
【分类号】：R744.51

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