有氧运动在预防小鼠2型糖尿病过程中对骨骼肌NO、SIRT1的影响

发布时间：2018-06-05 03:25

本文选题：有氧运动 + 2型糖尿病　；参考：《河北师范大学》2017年硕士论文

【摘要】：研究目的:目前,我国以及全球的2型糖尿病(T2DM)患病人数不断增加,引起了各界研究学者的普遍关注,由于T2DM很难治愈,因此寻找预防T2DM的方法显得尤为重要。规律的运动是促进代谢适应,防治多种代谢性疾病的有效手段。一氧化氮(NO)是一个无机分子,在细胞间充当信号分子,在生理学、生物化学、生物医学领域被广泛研究。沉默交配型信息调节因子2同源蛋白1(SIRT1)参与了葡萄糖/脂质代谢、线粒体生物合成等生理过程。最近有研究表明,运动可以通过NO/SIRT1介导的调控通路来改善T2DM患者的骨骼肌胰岛素敏感性,但在预防T2DM过程中,此通路的作用鲜有报道。本实验通过观察有氧运动干预后,高糖高脂膳食小鼠腓肠肌中一氧化氮合酶(NOS)活性、NO浓度和SIRT1蛋白表达变化,明确有氧运动刺激小鼠腓肠肌NOS/NO和SIRT1有调节空腹血糖、调节胰岛素敏感性和预防T2DM的重要作用,为预防T2DM提供新思路。研究方法:8周龄雄性Balb/Cj品系小鼠24只,随机分为:安静普通饲料组(CC组)、有氧运动普通饲料组(EC组)、高脂高糖膳食安静组(HC组)、高脂高糖膳食有氧运动组(HE组),每组6只。EC组和HE组的小鼠进行为期6周的跑台实验,速度从10m/min递增到15m/min,每一周递增一次速度(1m/min),每周5次,每次运动时间为45min,坡度为0。CC组和EC组进行普通饲料喂养,HC组和HE组进行高糖高脂膳食喂养。实验动物处死前,用罗氏血糖仪检测空腹血糖(FBG)水平,处死取材后,ELISA法检测空腹胰岛素(FIN)含量并计算出胰岛素敏感指数(ISI)和胰岛素抵抗指数(IR),采用生化试剂盒检测小鼠腓肠肌NOS活力、NO含量,Western blot法测定小鼠腓肠肌SIRT1蛋白表达。所得数据用SPSS20.0软件进行双因素方差分析。研究结果:(1)6周干预后,HC组小鼠的FBG水平和FIN含量均高于CC组,HE组、EC组分别均低于HC组、CC组,有氧运动和高糖高脂膳食干预对小鼠FBG水平的影响均显著(P0.01),对小鼠FIN含量的影响均显著(P0.01,P0.05);HC组小鼠的ISI低于CC组,HE组、EC组分别高于HC组、CC组,有氧运动和高糖高脂膳食干预对小鼠ISI的影响均显著(P0.01);HC组小鼠的IR高于CC组,HE组、EC组分别低于HC组、CC组,有氧运动和高糖高脂膳食干预对小鼠IR的影响均显著(P0.01)。(2)有氧运动和膳食干预对小鼠腓肠肌tNOS活力、iNOS活力的交互作用影响均显著(P0.01);HE组小鼠腓肠肌tNOS活力、iNOS活力均低于HC组(P0.01),EC组tNOS活力高于CC组,EC组iNOS活力与CC组无差异;有氧运动和高糖高脂膳食干预对小鼠腓肠肌中cNOS活力影响均显著(P0.01),HE组小鼠腓肠肌中cNOS活力高于HC组,EC组高于CC组。(3)有氧运动和高糖高脂膳食干预对小鼠腓肠肌NO含量的交互作用影响显著(P0.05);6周后,HE组低于HC组,有氧运动对高糖高脂膳食小鼠腓肠肌NO含量的影响显著(P0.05),对普通膳食小鼠无影响。(4)有氧运动和高糖高脂膳食干预对小鼠腓肠肌SIRT1蛋白表达的交互作用影响显著(P0.05),HE组小鼠腓肠肌SIRT1蛋白表达高于HC组,有氧运动对高糖高脂膳食小鼠腓肠肌SIRT1蛋白表达影响显著(P0.01),对普通膳食小鼠无影响。研究结论:(1)有氧运动降低了高糖高脂膳食小鼠的空腹血糖水平和空腹胰岛素含量,提高了高糖高脂膳食小鼠的胰岛素敏感性,降低了胰岛素抵抗。(2)有氧运动降低了高糖高脂膳食小鼠腓肠肌的tNOS活性,有助于缓解高糖高脂膳食小鼠的NO含量大量增加。(3)有氧运动显著提高了高糖高脂膳食小鼠腓肠肌SIRT1蛋白表达。
[Abstract]:Research purposes: at present, the number of patients with type 2 diabetes (T2DM) in China and the world is increasing, which has attracted the attention of researchers from all walks of life. Because T2DM is difficult to cure, it is very important to find a way to prevent T2DM. Regular exercise is an effective means to promote metabolic adaptation and prevent many kinds of metabolic diseases. Nitric oxide (NO) It is an inorganic molecule that acts as a signal molecule between cells and is widely studied in the field of physiology, biochemistry and biomedicine. The silent mating type information regulator 2 homologous protein 1 (SIRT1) participates in the physiological processes such as glucose / lipid metabolism and mitochondrial biosynthesis. The most recent studies show that exercise can be regulated by NO/SIRT1 mediated regulation. The pathway is used to improve the insulin sensitivity of skeletal muscle in T2DM patients, but in the process of preventing T2DM, the role of this pathway is rarely reported. In this experiment, the activity of nitric oxide synthase (NOS) in the gastrocnemius muscle of high glucose and high fat diet mice, the concentration of NO and the expression of SIRT1 egg white in the high fat and high fat diet mice were observed, and the NOS of the gastrocnemius muscle of mice was clearly stimulated by the aerobic exercise. /NO and SIRT1 have an important role in regulating fasting blood glucose, regulating insulin sensitivity and preventing T2DM, and providing new ideas for the prevention of T2DM. Methods: 8 weeks old male Balb/Cj strain mice were randomly divided into the quiet ordinary diet group (CC group), the aerobic exercise normal diet group (group EC), the high fat and high sugar diet quiet group (HC group), the high fat and high sugar diet. The oxygen exercise group (group HE), each group of 6.EC groups and HE groups of mice in group HE for 6 weeks of treadmill experiment, the speed increased from 10m/min to 15m/min, each week increased one time (1m/min), 5 times a week, each time was 45min, the slope was 0.CC and EC group feed, the HC and HE groups were fed high sugar and high fat diet. Experimental animals. Before death, the level of fasting blood glucose (FBG) was detected by the Roche glucose meter. After being killed, the content of fasting insulin (FIN) was detected by ELISA method and the insulin sensitivity index (ISI) and insulin resistance index (IR) were calculated. The NOS activity of the gastrocnemius, NO content and the expression of SIRT1 protein in the gastrocnemius muscle of mice were measured by the biochemical kit. The data were analyzed with SPSS20.0 software. The results were as follows: (1) the level of FBG and FIN in group HC were higher than that in group CC, group HE, group EC and CC group, and the effect of aerobic exercise and high glucose diet on FBG level of mice were all significantly (P0.01). 0.05): ISI of group HC mice was lower than group CC, HE group and EC group were higher than group HC, group CC, aerobic exercise and high sugar and high fat diet intervention were significant (P0.01), IR in HC group was higher than that of CC group, and the effect of aerobic exercise and high sugar diet intervention on mice was significant. (2) aerobic exercise. The effects of dynamic and dietary intervention on the activity of gastrocnemius tNOS and the interaction of iNOS activity were all significantly (P0.01); the tNOS activity of the gastrocnemius muscle in the group HE mice was lower than that of the HC group (P0.01), the tNOS activity of the EC group was higher than that of the CC group, and the activity of the EC group was not different from that of the CC group; the effects of aerobic exercise and high sugar and high fat diet on the activity of the calastus muscle in mice were all affected. Significantly (P0.01), the activity of cNOS in the gastrocnemius muscle of group HE was higher than that in group HC, and in group EC was higher than that in group CC. (3) the interaction of aerobic exercise and high sugar diet intervention on the NO content of the gastrocnemius muscle of mice was significantly affected (P0.05); after 6 weeks, the HE group was lower than the HC group, and the effect of aerobic exercise on the NO content of the gastrocnemius muscle in high glucose and high fat diet mice was significantly (P0.05). (4) the interaction of aerobic exercise and high fat diet intervention on the expression of SIRT1 protein in the gastrocnemius muscle of mice was significantly affected (P0.05). The expression of SIRT1 protein in the gastrocnemius muscle of group HE was higher than that of the HC group. The effect of aerobic exercise on the expression of gastrocnemius SIRT1 protein in high glucose and high fat diet mice was significant (P0.01), and no effect on the normal diet mice. Conclusions: (1) aerobic exercise reduces the fasting blood glucose level and fasting insulin content in high glucose and high fat diet mice, improves insulin sensitivity and reduces insulin resistance in high glucose and high fat diet mice. (2) aerobic exercise reduces the tNOS activity of the gastrocnemius in high glucose and high fat diet mice, and helps to alleviate the NO content of high glucose and high fat diet mice. (3) aerobic exercise significantly increased the expression of SIRT1 protein in gastrocnemius muscle of mice fed with high glucose and high-fat diet.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R587.1

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