新疆地区维吾尔族与汉族强直性脊柱炎患者的差异性基因表达

发布时间：2018-06-07 03:47

本文选题：组织工程 + 遗传　；参考：《中国组织工程研究》2016年15期

【摘要】：背景:目前强直性脊柱炎的发病机制尚不清楚,其发生可能与遗传因素、环境因素、慢性感染和自身免疫功能紊乱等因素有关。目前普遍认为遗传学因素在发病机制中起着重要作用,而且强直性脊柱炎的发病具有明显的家族聚集以及民族间的差异现象,目前寻找不同地区、不同民族之间强直性脊柱炎的差异性基因成为研究热点之一。目的:应用基因表达谱芯片技术筛选出当地维吾尔族与汉族强直性脊柱炎患者差异性基因,并对这些差异性基因进行比较分析。方法:随机采集新疆维吾尔自治区中医医院风湿科处于活动期的维吾尔族、汉族强直性脊柱炎患者各5例,另外分别选取各3例健康志愿者为对照,提取外周血中的RNA,制备探针后用于表达谱芯片杂交,筛选强直性脊柱炎组织差异表达基因,并采用半定量RT-PCR法验证芯片结果。结果与结论:通过对维吾尔族强直性脊柱炎与汉族强直性脊柱炎患者差异性基因表达研究发现,两组之间筛选出差异有显著性意义的miRNA 20个(P0.05);通过对维吾尔族强直性脊柱炎与汉族强直性脊柱炎患者靶基因与miRNA对应关系分析发现,相关的靶基因共有15个,这些靶基因相对应并存在一定的调控关系的miRNA有79个,这些基因主要为白细胞抗原类和白细胞介素类,均与人体免疫相关。结果证实,在中国新疆地区维吾尔族与汉族强直性脊柱炎患者之间,可筛选出与强直性脊柱炎相关多个差异表达基因。
[Abstract]:Background: at present, the pathogenesis of ankylosing spondylitis is not clear. The pathogenesis of ankylosing spondylitis may be related to genetic factors, environmental factors, chronic infection and autoimmune dysfunction. It is generally believed that genetic factors play an important role in the pathogenesis of ankylosing spondylitis, and the incidence of ankylosing spondylitis has obvious family aggregation and ethnic differences. The differentially expressed genes of ankylosing spondylitis among different nationalities have become one of the hotspots. Objective: to screen the differentially expressed genes of the local Uygur and Han nationality patients with ankylosing spondylitis by gene expression microarray and to compare these genes. Methods: five active Uygur and five Han ankylosing spondylitis patients were randomly collected from rheumatology department of Xinjiang Uygur Autonomous region traditional Chinese Medicine Hospital, and 3 healthy volunteers were selected as control. RNA was extracted from peripheral blood, and then the probe was used for microarray hybridization to screen differentially expressed genes in ankylosing spondylitis tissues. The microarray results were verified by semi-quantitative RT-PCR. Results & conclusion: the differential gene expression in patients with ankylosing spondylitis in Uygur and Han nationality was studied. A significant difference between the two groups of 20 miRNA P0.05A was screened, through the Uygur ankylosing spondylitis and Han nationality ankylosing spondylitis target gene and miRNA corresponding relationship analysis, found that there are 15 related target genes. There are 79 miRNA genes corresponding to these target genes. These genes are mainly leukocyte antigens and interleukins-related to human immunity. The results showed that multiple differentially expressed genes associated with ankylosing spondylitis could be screened between Uygur and Han patients in Xinjiang, China.
【作者单位】：新疆维吾尔自治区中医医院骨科;
【基金】：新疆维吾尔自治区自然科学基金项目(2013211B68)~~
【分类号】：R593.23

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