骨质疏松症患者肠道菌群多样性分析

发布时间：2018-06-07 23:18

本文选题：骨质疏松症 + 肠道菌群　；参考：《中国骨质疏松杂志》2017年06期

【摘要】：目的探讨原发性骨质疏松患者肠道菌群的多样性变化。方法收集原发性骨质疏松患者7例与健康对照组7例的粪便样本,提取肠道菌群DNA,16S rRNA基因扩增,Illumina平台测序。对测序结果进行物种注释、多样性分析、物种差异分析。结果原发性骨质疏松患者肠道菌群在纲、目、科、属、种、分类操作单位水平的菌群种类要多于对照组,且两组人群在纲、目、科、属、种、分类操作单位水平的肠道菌群种类有一定的重叠性;Alpha多样性指标中的赵氏指数及艾森指数在两组间差异具有统计学意义(P0.05),香农指数及辛普森指数在两组间差异无统计学意义(P0.05);Beta多样性中的层级聚类能够将两组样本进行鉴别区分;骨质疏松组与对照组样本在门、科、种分类水平的部分肠道菌群含量存在差异(P0.05),芽单胞菌门、绿弯菌门、酸杆菌门、芽单胞菌科、丛毛单胞菌科、厌氧绳菌科、毛螺菌、优杆菌等肠道菌群在骨质疏松患者中所占比例要大于正常对照组(P0.05)。结论 16S rRNA测序有助于分析原发性骨质疏松患者的肠道菌群多样性变化,为研究肠道菌群与骨质代谢的关系提供理论依据。
[Abstract]:Objective to investigate the diversity of intestinal flora in patients with primary osteoporosis. Methods fecal samples from 7 patients with primary osteoporosis and 7 healthy controls were collected, and the 16s rRNA gene of intestinal flora was amplified by Illumina platform sequencing. The sequencing results were analyzed by species annotation, diversity analysis and species diversity analysis. Results the intestinal flora of primary osteoporosis patients was more than that of the control group at the level of class, order, family, genus, species, classification and operation unit, and the two groups were in class, order, family, genus and species. There was a certain overlap between the two groups in the number of intestinal flora and the Issen index. There was statistical difference between the two groups, but there was no difference between the two groups in Shannon index and Simpson index. The statistical significance was that the hierarchical clustering in Beta diversity could distinguish the two groups of samples. There were significant differences in the intestinal flora between the osteoporosis group and the control group at the levels of phylum, family, species classification, P0.05, phylum, acidobacterium, Cytomonas, Cytomonidae, Anaerobicaceae, Helicobacter trichomes. The proportion of enteric bacteria such as eubacillus in osteoporosis patients was higher than that in normal control group (P 0.05). Conclusion 16s rRNA sequencing is helpful to analyze the diversity of intestinal flora in patients with primary osteoporosis and to provide a theoretical basis for the study of the relationship between intestinal flora and bone metabolism.
【作者单位】：西安交通大学医学院附属红会医院;电信科学技术第十研究所;
【基金】：国家自然科学基金(81601898) 西安市红会医院科研基金资助课题(YJ2016013) 中国博士后科学基金资助项目(2017M613176,2017M613177)
【分类号】：R580

【相似文献】