miRNA-126在支气管哮喘中的表达及临床意义

发布时间：2018-06-12 22:15

本文选题：miRNA- + 哮喘　；参考：《中国老年学杂志》2017年11期

【摘要】：目的探讨检测miRNA-126基因在支气管哮喘患者及健康者外周血中的表达及在哮喘急性加重期的临床意义。方法哮喘急性加重期患者24例为哮喘组,健康志愿者24例为对照组。留取外周血标本,采用实时荧光定量PCR(RT-PCR)检测miRNA-126基因的表达,分析其表达差异情况以及与白细胞介素(IL)-6细胞炎症因子、嗜酸性细胞、IgE的关系,受试者工作特征曲线(ROC)特征评价miRNA-126基因对支气管哮喘诊断的预测价值。结果两组miRNA-126相对含量差异有统计学意义(Z=-2.444,P=0.015)。哮喘组IL-6、嗜酸性细胞、IgE比率显著高于对照组(P0.01)。miR-126在支气管哮喘患者血浆中ROC曲线下面积(AUC)为0.294,诊断价值较低。结论 miRNA-126在哮喘急性加重期患者外周血中表达,呈下调状态,可能通过辅助T细胞(Th)2优势应答,使炎性细胞因子升高,有可能成为治疗哮喘的新靶点。
[Abstract]:Objective to investigate the expression of miRNA-126 gene in peripheral blood of asthmatic patients and healthy subjects and its clinical significance in acute exacerbation of asthma. Methods Twenty-four patients with acute exacerbation of asthma were treated with asthma and 24 healthy volunteers as control group. The expression of miRNA-126 gene was detected by real-time fluorescence quantitative PCR-RT-PCR in peripheral blood samples, and the relationship between the expression of miRNA-126 gene and the inflammatory factor and eosinophilic cell IgE in IL-6 cells was analyzed. Objective: to evaluate the predictive value of miRNA-126 gene in the diagnosis of bronchial asthma. Results the difference of the relative content of miRNA-126 between the two groups was statistically significant. The ratio of IL-6 and eosinophil IgE in asthma group was significantly higher than that in control group (P 0.01). The area under ROC curve was 0.294, and the diagnostic value was lower. Conclusion the expression of miRNA-126 in peripheral blood of patients with acute exacerbation of asthma is down-regulated and may be a new target for the treatment of asthma.
【作者单位】：新疆医科大学第二附属医院;
【基金】：新疆医科大学科研创新基金项目(No.XYDCX201435)
【分类号】：R562.25

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