miR-23b-3p对3T3-L1前脂肪细胞增殖和成脂分化的影响

发布时间：2018-06-20 16:19

本文选题：miR-b-p + T-L前脂肪细胞　；参考：《中国兽医学报》2017年10期

【摘要】：为探讨miR-23b-3p对3T3-L1前脂肪细胞增殖和成脂分化的影响,通过化学修饰的miR-23b-3p模拟物成功转入3T3-L1前脂肪细胞后,利用CCK-8法和流式细胞术检测过表达miR-23b-3p对3T3-L1前脂肪细胞增殖的影响;并通过实时荧光定量PCR和Western blot检测细胞周期基因CyclinD1和CDK4以及成脂标志基因PPARγ和FABP4的表达情况;油红O染色测定甘油三酯积累情况。结果显示,过表达miR-23b-3p抑制3T3-L1前脂肪细胞增殖,导致细胞G1期阻滞,CyclinD1、CDK4在mRNA和蛋白水平极显著下调;在3T3-L1前脂肪细胞成脂分化过程中,miR-23b-3p的表达水平上调;过表达miR-23b-3p促进3T3-L1前脂肪细胞甘油三酯积累,PPARγ、FABP4在mRNA和蛋白水平显著或极显著上调。结果表明,miR-23b-3p能够抑制3T3-L1前脂肪细胞增殖并促进其分化为成熟脂肪细胞。
[Abstract]:In order to investigate the effect of miR-23b-3p on the proliferation and adipogenic differentiation of 3T3-L1 preadipocytes, the chemically modified miR-23b-3p mimics were successfully transferred into 3T3-L1 preadipocytes. The effects of miR-23b-3p expression on the proliferation of 3T3-L1 preadipocytes were detected by CCK-8 and flow cytometry. The expression of cyclin D1 and CDK4, lipogenic marker PPAR 纬 and FABP4 were detected by real-time fluorescence quantitative PCR and Western blot, and the accumulation of triglyceride was detected by oil red O staining. The results showed that the overexpression of miR-23b-3p inhibited the proliferation of 3T3-L1 preadipocytes and led to the down-regulation of cyclin D1 CDK4 at the mRNA and protein levels, and the up-regulation of miR-23b-3p expression during the adipogenic differentiation of 3T3-L1 preadipocytes. Overexpression of miR-23b-3p promoted the accumulation of triglyceride in 3T3-L1 preadipocytes. The results showed that miR-23b-3p could inhibit the proliferation of 3T3-L1 preadipocytes and promote their differentiation into mature adipocytes.
【作者单位】：华南农业大学兽医学院;
【基金】：国家自然科学基金资助项目(31272519)
【分类号】：R589.2

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