连续和间断运动对高脂饮食肥胖大鼠胸主动脉血管反应性影响的研究

发布时间：2018-06-21 07:03

本文选题：连续运动 + 间断运动　；参考：《宁夏医科大学》2015年硕士论文

【摘要】：目的本实验以高脂饮食肥胖大鼠为模型,通过连续和间断运动(continuous and intermittent exercise)干预,观察连续和间断运动对高脂饮食肥胖大鼠胸主动脉血管反应性的影响,以期初步阐明高脂饮食中引起心血管功能损伤的可能机制,也为进一步开发连续和间断运动提供实验依据和理论指导。方法将雄性SD大鼠48只,随机分成6组,普通饮食组(conventional diet,CD)、普通饮食连续运动组(conventional with continuous exercise group,CCE)、普通饮食间断运动组(conventional with intermittent exercise group,CIE)、高脂饮食组(high-fat diet,HD)、高脂饮食连续运动组(high-fat diet with continuous exercise,HCE)、高脂饮食间断运动组(high-fat diet with intermittent exercise,HIE)。进行8周游泳训练后,观察大鼠体重、食物的利用率、Lee’s指数、体长的变化;采用血管环组织浴槽离体技术测定去甲肾上腺素(Noradrenaline,NA)对大鼠胸主动脉血管的反应性;检测血清中超氧化物歧化酶(Super oxide dismutase,SOD)、丙二醛(Malondialdehyde,MDA)、一氧化氮(Nitric oxide,NO)的变化,低密度脂蛋白(low density lipoprotein,LDL)、胆固醇(Cholesterol,CHOL)、甘油三酯(Triglyceride,TG)、高密度脂蛋白(High-density lipoprotein,HDL);检测大鼠血管胸主动脉一氧化氮合酶(Endothelial nitric oxide synthase,e NOS)基因的表达水平;观察胸主动脉形态学变化。结果1.各组大鼠体重均逐周增长,均有统计学意义(P0.01)。2.各组大鼠体长均逐渐增长,与普通饮食组比较,高脂饮食组体长略长一些(P0.05),而普通饮食连续运动和间断运动组无统计学意义(P0.05);与高脂饮食组比较,高脂饮食间断运动组体长略小一些(P0.05),高脂饮食连续运动组和高脂饮食间断运动组比较,无统计学意义(P0.05)。3.高脂饮食组大鼠食物利用率显著高于普通饮食组(P0.01),普通饮食连续运动组和间断运动组食物利用率显著低于高脂饮食组(P0.01),高脂饮食连续运动组和高脂饮食间断运动组食物利用率低于高脂饮食组(P0.01);而连续运动和间断运动组比较,无统计学意义(P0.05)。4.高脂饮食组LEE’S指数明显高于普通饮食组(P0.01),普通饮食连续运动和间断动和间断运动组LEE’S指数低于高脂饮食组(P0.01),但高脂饮食连续运动组和高脂饮食间断运动组LEE’S指数比较,无统计学差异(P0.05)。5.高脂饮食组大鼠胸主动脉血管环对NA的收缩反应高于普通饮食组(P0.01);与高脂饮食组相比,高脂饮食连续运动组和高脂饮食间断运动组对NA的收缩反应有所降低,有显著性差异(P0.01),与高脂饮食连续运动组比较,高脂饮食间断运动组对NA的收缩反应降低更明显(P0.01);与普通饮食组比较,普通饮食连续运动组和普通饮食间断运动组也有所下降(P0.01),而与普通饮食连续运动组比较,普通饮食间断运动组略下降(P0.05)。6.普通饮食连续运动组和普通饮食间断运动组血清中CHOL、TG、LDL含量低于普通饮食组(P0.05),高脂饮食组血清中CHOL、TG、LDL含量高于普通饮食组(P0.01),高脂饮食连续运动组和高脂饮食间断运动组CHOL、TG、LDL低于高脂饮食组(P0.01),间断运动组血清中CHOL、TG、LDL含量低于连续运动组(P0.01);高脂饮食组血清中HDL的含量低于普通饮食组,无统计学意义(P0.05),普通饮食连续运动组和间断运动组血清中HDL的含量略高于普通饮食组,无统计学意义(P0.05);而高脂饮食连续运动组和间断运动组HDL含量高于高脂饮食组(P0.01),间断运动组和连续运动组HDL含量比较并无统计学差异(P0.05)。与普通饮食组比较,高脂饮食组NO含量减少(P0.01),普通饮食连续和间断运动组NO含量并无明显变化(P0.05);与高脂饮食组比较,高脂饮食连续运动和间断运动组NO含量略上升,但无统计学意义(P0.05)。7.高脂饮食组心肌中MDA含量高于普通饮食组(P0.01),普通饮食连续运动组和间断运动组心肌中MDA含量低于普通饮食组(P0.01),而普通饮食间断运动组心肌中MDA含量比普通饮食连续运动组下降得更明显(P0.05);高脂饮食连续运动和间断运动组心肌中MDA含量低于高脂饮食组(P0.01),而高脂饮食连续运动组和高脂饮食间断运动组并无差异(P0.05)。与普通饮食组比较,高脂饮食组心肌中SOD含量下降(P0.01),普通饮食连续运动组和间断运动组心肌中SOD含量上升(P0.01),普通饮食连续运动组和间断运动组比较并无统计学差异(P0.05);和高脂饮食组比较,高脂饮食连续运动和间断运动组心肌中SOD含量上升(P0.01),而高脂饮食连续运动组和高脂饮食间断运动组比较也无统计学差异(P0.05)。8.普通饮食组eNOS mRNA基因表达水平高于高脂饮食组(P0.05),与普通饮食组相比,普通饮食连续运动组和间断运动组胸主动脉血管e NOS m RNA基因表达有上调趋势,但无统计学意义(P0.05);普通饮食连续运动组和普通饮食间断运动组比较无统计学差异(P0.05);高脂饮食连续运动和间断运动组中胸主动脉血管e NOS m RNA基因的表达量显著高于高脂饮食组(P0.05),而高脂饮食间断运动与高脂饮食连续运动组比较无统计学差异(P0.05)。结论连续运动和间断运动具有改善高脂饮食肥胖大鼠动脉血管反应性的作用;增加血清抗氧化酶活力,减少心肌自由基的代谢及改善血清脂质代谢,使e NOS m RNA的表达上调,增加NO含量,间断运动方式总体来说改善的更好。
[Abstract]:Objective To observe the effect of continuous and intermittent movement (continuous and intermittent exercise) on the vascular reactivity of thoracic aorta in high fat diet obese rats by continuous and intermittent movement (intermittent exercise), and to clarify the possible mechanism of cardiovascular damage in high fat diet. The experimental basis and theoretical guidance were provided for further development of continuous and intermittent movement. Methods 48 male SD rats were randomly divided into 6 groups, ordinary diet group (conventional diet, CD), common diet continuous exercise group (conventional with continuous exercise group, CCE), common diet intermittent exercise group (conventional with) Group, CIE), the high fat diet group (high-fat diet, HD), the high fat diet continuous exercise group (high-fat diet with continuous exercise, HCE), and the high fat diet intermittent exercise group (high-fat). After swimming training, the body weight, the utilization rate of food, the change of body length, the use of blood vessels and the blood vessel were observed. The reactivity of Noradrenaline (NA) to the aorta of thoracic aorta in rats was measured in vitro. The changes in serum superoxide dismutase (Super oxide dismutase, SOD), malondialdehyde (Malondialdehyde, MDA), nitric oxide (Nitric oxide, NO), low density lipoprotein (low), gallbladder, and bile solid were detected. Alcohol (Cholesterol, CHOL), triglyceride (Triglyceride, TG), high density lipoprotein (High-density lipoprotein, HDL), the expression level of nitric oxide synthase (Endothelial nitric oxide synthase, e) gene in the thoracic aorta of rats and the morphological changes of the thoracic aorta were observed. Results 1. groups of rats were all body weight per week. In P0.01.2., the body length of rats in each group increased gradually. Compared with the normal diet group, the high fat diet group was slightly longer (P0.05), while the continuous exercise and the intermittent exercise group had no statistical significance (P0.05). Compared with the high fat diet group, the body length of the high fat diet group was slightly smaller (P0.05) and the high fat diet continuous exercise group was more than that of the high fat diet group. Compared with the high fat diet intermittent exercise group, the food utilization rate of the.3. high fat diet group was significantly higher than that of the normal diet group (P0.01). The food utilization rate of the continuous exercise group and the intermittent exercise group was significantly lower than that of the high fat diet group (P0.01). The food in the high fat diet continuous exercise group and the high fat diet intermittent exercise group were better than those in the high fat diet group (P0.01). The use rate was lower than the high fat diet group (P0.01), while the continuous exercise and intermittent exercise group had no statistical significance (P0.05) the LEE 'S index of the high fat diet group was significantly higher than that of the normal diet group (P0.01). The LEE' S index of the regular diet and discontinuous exercise group was lower than the high fat diet group (P0.01), but the high fat diet continuous exercise group and the high fat diet group were higher than the high fat diet group (P0.01), but the high fat diet group and the high fat diet group were higher than the high fat diet group (P0.01). There was no statistical difference in the LEE 'S index in the discontinuous exercise group (P0.05). The contraction response of the thoracic aorta ring to the NA was higher in the.5. high fat diet group than that of the normal diet group (P0.01). Compared with the high fat diet group, the contraction reaction of the high fat diet continuous exercise group and the high fat diet intermittent movement group was lower than the high fat diet group (P0). (P0 .01), compared with the high fat diet continuous exercise group, the contractile response of NA in the high fat diet group decreased significantly (P0.01). Compared with the ordinary diet group, the continuous exercise group and the normal diet discontinuous exercise group also decreased (P0.01), but compared with the regular diet exercise group, the normal diet intermittent exercise group decreased slightly (P0.05 The content of CHOL, TG, and LDL in the serum of the.6. diet continuous exercise group and the normal diet discontinuous exercise group was lower than that of the ordinary diet group (P0.05). The content of CHOL, TG, LDL in the serum of high fat diet group was higher than that of the ordinary diet group (P0.01). The high fat diet continuous exercise group and the high fat diet break exercise group were CHOL, TG, LDL was lower than the high fat diet group and intermittent exercise group. The content of CHOL, TG and LDL in the serum was lower than that of the continuous exercise group (P0.01), and the content of HDL in the serum of high fat diet group was lower than that of the ordinary diet group (P0.05). The content of HDL in the serum of the normal diet continuous exercise group and the intermittent exercise group was slightly higher than that of the ordinary diet group (P0.05), but the high fat diet continuous exercise group and the intermittent transport group were not statistically significant (P0.05). The content of HDL in the dynamic group was higher than that of the high fat diet group (P0.01), and there was no significant difference in the HDL content between the intermittent exercise group and the continuous exercise group (P0.05). Compared with the normal diet group, the NO content of the high fat diet group decreased (P0.01), and the NO content in the normal diet continuous and discontinuous exercise group had no obvious changes (P0.05), and the high fat diet was compared with the high fat diet group. The content of NO in the dynamic and intermittent exercise groups was slightly higher, but the content of MDA in the myocardium of the high fat diet group was higher than that of the ordinary diet group (P0.01). The content of MDA in the myocardium of the normal diet continuous exercise group and the intermittent exercise group was lower than that of the normal diet group (P0.01), while the content of MDA in the myocardium of the normal diet group was more than that of the normal diet group (P0.01). The content of MDA in the normal diet group was more than that of the normal diet group (P0.01). The MDA content in the myocardium of the high fat diet continuous exercise and the intermittent exercise group was lower than that of the high fat diet group (P0.01), but there was no difference between the high fat diet continuous exercise group and the high fat diet intermittent exercise group (P0.05). Compared with the normal diet group, the SOD content decreased (P0.01) in the high fat diet group (SOD) and the normal diet continuous exercise group. There was no significant difference in the SOD content in the myocardium in the intermittent exercise group (P0.01), and there was no statistical difference between the continuous exercise group and the intermittent exercise group (P0.05). Compared with the high fat diet group, the SOD content in the continuous and intermittent exercise groups of the high fat diet increased (P0.01), while the high fat diet continuous exercise group and the high fat diet intermittent exercise group were also compared. There was no statistical difference (P0.05).8. common diet group eNOS mRNA gene expression level is higher than the high fat diet group (P0.05), compared with the ordinary diet group, the common diet continuous exercise group and intermittent exercise group of thoracic aorta, e NOS m RNA gene expression up-regulated, but no statistical significance (P0.05); common diet continuous exercise group and ordinary diet. There was no statistical difference in the discontinuous exercise group (P0.05); the expression of the e NOS m RNA gene in the thoracic aorta in the high fat diet continuous exercise group and the intermittent exercise group was significantly higher than that in the high fat diet group (P0.05), while the high fat diet intermittent movement and the high fat diet continuous exercise group had no statistical difference (P0.05). Conclusion continuous exercise and intermittent exercise have no statistical difference (P0.05). Improve arterial responsiveness of rat with high fat diet effect; increase the antioxidant activity of serum, reduce myocardial free radical metabolism and improve serum lipid metabolism, increase the expression of RNA e NOS m, increase the content of NO, generally intermittent exercise better.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589.2

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