促红细胞生成素在大鼠急性一氧化碳中毒迟发型脑病中的表达及意义

发布时间：2018-06-23 01:59

本文选题：急性一氧化碳中毒迟发型脑病 + Morris水迷宫　；参考：《实用医学杂志》2017年19期

【摘要】：目的探讨促红细胞生成素(EPO)在大鼠急性一氧化碳中毒迟发型脑病(DEACMP)中的表达及意义。方法将90只SD级雄性健康成年大鼠按简单随机化方法随机分为3组:空白对照组(BC组)、空气对照组(AC组)、DEACMP组。DEACMP组采用腹腔注射一氧化碳法建立DEACMP模型,AC组在腹腔内注射等量空气,BC组不作任何处理。将3个组按染毒后第1、3、7、14、21、28天分为6个亚组。运用Morris水迷宫实验检测大鼠认知行为学改变,TUNEL染色观察大鼠海马CA1区细胞凋亡情况,免疫组化染色观察大鼠海马CA1区EPO的表达。结果 DEACMP组大鼠平均逃避潜伏期较BC组和AC组明显延长,差异有统计学意义(P0.05)。DEACMP组凋亡指数(AI)在染毒后第1天开始逐渐升高,第7天达峰值,并保持较高水平持续到第28天,各时间点均高于BC组和AC组,差异有统计学意义(P0.05)。DEACMP组EPO蛋白的表达从染毒后的第1天开始有较明显的升高,在染毒后第3天达到峰值,第7天后开始逐渐降低,各时间点EPO表达高于BC组和AC组,差异有统计学意义(P0.05)。结论 EPO在急性一氧化碳中毒中后期表达降低、抗凋亡作用减弱可能是DEACMP发病原因之一。
[Abstract]:Objective to investigate the expression and significance of erythropoietin (EPO) in rats with delayed encephalopathy (DEACMP) with acute carbon monoxide poisoning (DEACMP). Methods 90 male healthy adult rats were randomly divided into 3 groups according to the simple randomization method: blank control group (group BC), air control group (AC group), and group DEACMP.DEACMP group by intraperitoneal injection of carbon monoxide method The DEACMP model was established, group AC was injected with equal amount of air in the abdominal cavity and no treatment was done in group BC. The 3 groups were divided into 6 subgroups according to the 1,3,7,14,21,28 days after exposure. The Morris water maze test was used to detect the change of cognitive behavior in rats. TUNEL staining was used to observe the apoptosis in the hippocampus CA1 region, and the EPO rat hippocampus CA1 area EPO was observed by immunohistochemistry. Results the average escape latency of the DEACMP group was significantly longer than that of the BC group and the AC group. The difference was statistically significant (P0.05) in.DEACMP group, the apoptosis index (AI) began to increase gradually at first days after exposure, and the peak value of seventh Tianda remained high and maintained at a high level to twenty-eighth days. All time points were higher than those of the BC group and AC group, the difference was statistically significant (P0.05). The expression of EPO protein in group.DEACMP began to rise obviously from the first day after exposure. It reached a peak at third days after exposure and gradually decreased in seventh days. The expression of EPO in each time point was higher than that in group BC and AC group. The difference was statistically significant (P0.05). Conclusion the expression of EPO decreased in the middle and late stage of acute carbon monoxide poisoning and the anti apoptosis effect may be weakened. It is one of the causes of DEACMP.
【作者单位】：四川省泸州市人民医院神经内科;重庆市璧山区人民医院神经内科;西南医科大学附属医院神经内科;
【基金】：四川省卫生厅科研基金项目(编号:09095)
【分类号】：R595.1;R747.9

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