强直性脊柱炎患者外周血T细胞亚群及相关免疫分子的变化

发布时间：2018-06-25 04:31

本文选题：强直性脊柱炎 + 免疫细胞　；参考：《现代免疫学》2017年01期

【摘要】：为探究强直性脊柱炎(ankylosing spondylitis,AS)的发病机制和免疫细胞在病情发展中的功能和重要性,我们设计本实验以比较强直性脊柱炎患者与正常人外周血中免疫细胞格局的变化。实验以42例AS患者和42例健康者为研究对象,检测外周血T细胞以及相应的细胞因子和转录因子。实验采用流式细胞术检测T细胞亚群的比例、通过ELISA方法检测细胞因子并使用Q-PCR检测转录因子的表达。实验发现,与健康人相比,AS患者外周血T细胞中CD4~+细胞占优势,并且effect T细胞与na?ve T细胞的比值升高;对CD4~+T细胞亚群进一步的检测结果显示Th1与Tfh的比例显著增高,Treg细胞的比例较正常人稍高。在对细胞因子的检测中,我们发现IFN-γ、TGF-β显著下降,但IL-21有上升趋势;对转录因子的检测显示,Bcl-6/Foxp3、RORγt/Foxp3和T-bet/Foxp3值均升高。上述结果提示强直性脊柱炎患者外周血中T细胞亚群的格局发生了变化,其相关免疫分子也呈现免疫活化状态。
[Abstract]:In order to investigate the pathogenesis of ankylosing spondylitis (as) and the function and importance of immune cells in the development of the disease, we designed this experiment to compare the pattern of immune cells in peripheral blood between patients with ankylosing spondylitis and normal subjects. Peripheral blood T cells, cytokines and transcription factors were measured in 42 patients with as and 42 healthy subjects. Flow cytometry was used to detect the proportion of T cell subsets, Elisa was used to detect cytokines and Q-PCR was used to detect the expression of transcription factors. It was found that CD4- cells in peripheral blood T cells were dominant in patients with as compared with healthy controls, and the ratio of effect T cells to na?ve T cells was increased. Further detection of CD4 ~ T cell subsets showed that the ratio of Th1 to Tfh was significantly higher than that of normal controls. In the detection of cytokines, we found that IFN- 纬 TGF- 尾 decreased significantly, but IL-21 increased, and transcription factor detection showed that Bcl-6 / Foxp3 ROR 纬 -t / Foxp3 and T-betr / Foxp3 increased. The above results suggest that the pattern of T cell subsets in peripheral blood of patients with ankylosing spondylitis has changed, and the related immune molecules are also in the state of immune activation.
【作者单位】：上海交通大学医学院免疫学与微生物学系;
【基金】：国家级大学生创新创业训练计划项目(201610248072) 上海交通大学医学院第九期大学生科技创新项目(201610248072);上海交通大学医学院2013级PBL项目
【分类号】：R593.23

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