丹参提取物促进骨髓间充质干细胞增殖及成骨分化的研究

发布时间：2018-06-25 14:37

  本文选题：丹参提取物 + 骨髓间充质干细胞　； 参考：《中国骨质疏松杂志》2017年08期

【摘要】：目的研究丹参提取物对骨髓间充质干细胞增殖及成骨分化的作用。方法采用全骨髓细胞接种法和贴壁纯化培养骨髓间充质干细胞,将浓度为0.2、0.4、0.8、1.6 mg/m L和3.2 mg/m L的丹参提取物加入到骨髓间充质干细胞中,观察不同浓度的丹参提取物对骨髓间充质干细胞的增殖作用;采用0.4、0.8 mg/m L和3.2 mg/m L的丹参提取物诱导骨髓间充质干细胞向成骨细胞分化,连续诱导21 d,利用ALP试剂盒测定成骨细胞内ALP活性和茜素红染色观察成骨细胞钙化结节情况;采用Western bloting法测定成骨细胞相关蛋白BMP-2和Runx的含量。结果不同浓度的丹参提取物均能够促进骨髓间充质干细胞的增殖,丹参提取物浓度0.8 mg/m L时对骨髓间充质干细胞的增殖作用最强;ALP测定结果发现0.4、0.8 mg/m L和3.2mg/m L的丹参提取物均能够提高ALP活性,丹参提取物浓度0.8 mg/m L时ALP活性最高;茜素红染色结果发现0.4、0.8 mg/m L和3.2 mg/m L丹参提取物均能够促进成骨细胞的钙化,丹参提取物浓度为0.8 mg/m L时钙化结节数量最多;Western bloting结果表明,0.8 mg/m L的丹参提取物能够促进成骨细胞分化相关蛋白BMP-2和Runx-2表达。结论丹参提取物能够促进大鼠骨髓间充质干细胞的增殖和成骨细胞分化作用。
[Abstract]:Objective to study the effects of Salvia miltiorrhiza extract on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. Methods Bone marrow mesenchymal stem cells (BMSCs) were cultured by whole bone marrow cell inoculation and adherent method. The extracts of Salvia miltiorrhiza (0.2G) 0.40.81.6 mg/m / L and 3.2 mg/m / L were added to bone marrow mesenchymal stem cells (BMSCs). The proliferation of bone marrow mesenchymal stem cells (BMSCs) was observed with different concentrations of Salvia miltiorrhiza extract, and the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts was induced by 0.40.8-and 3.2 mg/m / L Danshen extract. ALP activity and alizarin red staining were used to detect calcified nodules in osteoblasts and the contents of BMP-2 and Runx in osteoblasts were determined by Western bloting assay. Results different concentrations of Salvia miltiorrhiza extract could promote the proliferation of bone marrow mesenchymal stem cells. The proliferation of bone marrow mesenchymal stem cells was the strongest when the extract concentration of Salvia miltiorrhiza was 0. 8 mg/m / L. The results showed that the extract of 0. 4 ~ 0. 8 mg/m / L and 3.2mg/m / L could increase the activity of ALP, and the concentration of 0. 8 mg/m / L of Salvia miltiorrhiza had the highest activity of ALP. The results of alizarin red staining showed that 0.40.8 mg/m / L and 3.2 mg/m / L Salvia miltiorrhiza extracts could promote the calcification of osteoblasts. When the extract concentration of Salvia miltiorrhiza was 0. 8 mg/m / L, the number of calcified nodules was the highest. The results showed that the extract of Salvia miltiorrhiza at 0. 8 mg/m / L could promote the expression of BMP-2 and Runx-2 proteins associated with osteoblast differentiation. Conclusion Salvia miltiorrhiza extract can promote the proliferation and osteoblast differentiation of rat bone marrow mesenchymal stem cells.
【作者单位】： 牡丹江医学院红旗医院骨科;牡丹江医学院内分泌科;牡丹江医学院;
【分类号】：R580
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本文编号：2066346