维生素D对活动期系统性红斑狼疮患者内皮祖细胞的影响

发布时间：2018-06-26 20:53

  本文选题：红斑狼疮 + 系统性　； 参考：《暨南大学学报(自然科学与医学版)》2017年06期

【摘要】：目的:观察活动期系统性红斑狼疮(SLE)患者内皮祖细胞(EPCs)数量及功能的变化,并探讨维生素D对SLE患者EPCs数量及功能的影响.方法:收集30例SLEDAI评分大于8分,病情均处于活动期的SLE患者,30例年龄与性别匹配的健康人作为正常对照;酶联免疫吸附试验(ELISA)检测外周血25羟基维生素D[25(OH)D]水平;密度梯度离心和贴壁培养法分离培养EPCs,流式细胞术检测CD34~+/VEGFR-2~+EPCs在全血中的比例;通过计数再贴壁和构建侵袭小室检测EPCs黏附和迁移能力.结果:(1)体外培养过程中,活动期SLE患者EPCs数量(0.028±0.017)%显著低于正常对照组(0.067±0.012)%,有统计学差异(P0.05);(2)活动期SLE患者EPCs迁移率(1.7±0.9)‰及黏附能力(19±7)显著低于正常对照组EPCs迁移率(3.1±1.6)‰及黏附能力(34±11),有统计学差异(P0.05);(3)活动期SLE患者外周血25(OH)D质量浓度(14.47±10.39)ng/mL水平低于健康对照组质量浓度(24.15±7.98)ng/mL,有统计学差异(P0.05);(4)25(OH)D能够增加活动期SLE患者EPCs数量(0.045±0.012)%,高于未加25(OH)D组(0.031±0.012)%,有统计学差异(P0.05);(5)25(OH)D能够增加活动期SLE患者EPCs迁移率(2.6±0.7)‰及黏附能力(24±9),高于未加25(OH)D组迁移率(1.3±0.8)‰及黏附能力(13±6),有统计学差异(P0.05).结论:SLE患者维生素D水平降低,可诱发Ⅰ型干扰素通路活化,从而导致EPCs数量和/或功能异常,最终造成狼疮患者血管内皮损伤后修复障碍引发动脉粥样硬化.
[Abstract]:Aim: to observe the changes of endothelial progenitor cells (EPCs) in active systemic lupus erythematosus (SLE) patients, and to investigate the effect of vitamin D on the number and function of EPCs in SLE patients. Methods: 30 SLE patients with SLEDAI score more than 8 and 30 healthy subjects matched with age and sex were collected as normal control, and the levels of 25 hydroxyvitamin D (25 (OH) D) in peripheral blood were detected by Elisa. EPCs were isolated and cultured by density gradient centrifugation and adherent culture. CD34 ~ / VEGFR-2 ~ EPCs were detected by flow cytometry, and the adhesion and migration of EPCs were detected by counting and reattachment and building invasive chambers. Results: (1) in vitro culture, The number of EPCs in active SLE patients (0.028 卤0.017)% was significantly lower than that in normal controls (0.067 卤0.012). There was statistical difference (P0.05) between active SLE patients and controls (P0.05). The mobility and adhesion ability of active); (patients were significantly lower than those of normal controls (3.1 卤1.6 鈥，

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