咖啡豆提取物对营养肥胖型大鼠减肥的作用及机制初探

发布时间：2018-06-27 18:11

本文选题：咖啡豆提取物 + 脂联素　；参考：《浙江工业大学》2015年硕士论文

【摘要】：目的:比较两种咖啡豆提取物(CBE(一)和CBE(二))对营养肥胖模型大鼠的减肥作用,并对其中CBE(一)的减肥的作用机制进行了初步研究,为临床应用作前期基础研究。方法:按“国食药监保化[2012]107号”文附件8减肥功能评价方法进行造模并给药,观察给药组与模型对照组(MC)的情况,记录CBE(一)和CBE(二)各组大鼠的体重变化、摄食量;试验结束,测定各组大鼠体内脂肪重量、全自动生化仪测定CBE(一)血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的值;用Elisa法检测脂联素(APN)、瘦素(LP);试剂盒检测游离脂肪酸(NEFA)的含量;用Western blot法检测各组大鼠肝脏组织AdipoR2、p38、PGC-1、PPARγ蛋白的表达和脂肪组织中AdipoR2、PGC-1、PPARγ蛋白的表达以及肌肉组织中AdipoR2、PGC-1的表达;用RT-PCR法测定大鼠肝脏组织中AdipoR2、p38、PPARγ、SREBF1、SREBF2基因表达水平。结果:大鼠营养肥胖模型成功建立,两种咖啡豆提取物均有减肥作用。具体表现在:与正常对照组(NC)大鼠比较,模型对照组(MC)的体重终重、体重增重和脂体比有明显差异(P0.01);与MC比较,两种给药组大鼠体重终重、体重增重和脂体比均下降,均数间差异均有显著性(P0.01)。与NC比较,MC大鼠TC、TG、LDL均升高,HDL含量下降,差异有显著性(P0.01);与MC比较,CBE(一)(133mg·kg-1、267 mg·kg-1、800 mg·kg-1)三个剂量组TC、TG均降低,HDL均升高,其中133 mg·kg-1、267 mg·kg-1两个剂量组LDL无明显变化,而800 mg·kg-1组LDL降低(P0.05)。同时与NC相比,MC大鼠APN均明显降低(P0.01),LP增多;与MC相比,CBE(一)给药组大鼠APN增多(P0.01或0.05),LP降低。Western blot实验表明,与MC相比,CBE(一)给药组大鼠肝脏组织中AdipoR2、p38、PGC-1、PPARγ蛋白表达增多,脂肪组织中AdipoR2、PGC-1、PPARγ蛋白表达增多,而肌肉组织中AdipoR2、PGC-1蛋白表达增多,未检测出PPARγ蛋白的表达。RT-PCR结果表明,大鼠肝脏SREBF1、SREBF2 mRNA显著降低,而AdipoR2、p38、PPARγmRNA显著增多。结论:按“国食药监保化[2012]107号”文附件8减肥功能评价方法进行试验,两种咖啡豆提取物均有减肥作用,其中其作用机制可能是通过升高血清APN水平,降低LP水平,PGC-1表达增加,在一定程度上上调PPARγ的水平,从而促进脂肪组织释放APN,增加APN与Adipo R2结合激活下游的AMPKα、p38、PPARα,下调SREBF1,从而促进脂肪的分解,抑制脂肪的生成,达到减肥的效果。
[Abstract]:Objective: to compare the effects of two kinds of coffee bean extracts (CBE (1) and CBE (2) on the weight loss of nutritional obese rats. Methods: according to the evaluation method of weight-loss function in annex 8 of "National Food and Drug Administration Supervision, Baohua [2012] 107", the changes of body weight and food intake were observed between the administration group and the model control group (MC), and the changes of body weight and the food intake were recorded in each group (CBE (1) and CBE (2). Total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL) in the serum of CBE (I) were measured by automatic biochemical instrument. Adiponectin (APN), leptin (LP), free fatty acid (NEFA) were detected by Elisa, AdipoR2P38-PGC-1PPAR 纬 protein and AdipoR2PGC-1PPAR 纬 protein were detected by Western blot assay, and AdipoR2PGC-1PPAR 纬 protein in muscle tissue were detected by Western blot assay. The expression of AdipoR2 (p38) PPAR 纬 -SREBF1 (SREBF2) gene in rat liver was determined by RT-PCR. Results: the model of nutritional obesity in rats was established successfully. Compared with the normal control group (NC), the model control group (MC) had a significant difference in body weight, weight gain and fat body ratio (P0.01), and compared with MC, the weight gain, weight gain and lipids weight ratio of the two groups were decreased, and compared with the control group, the weight gain and fat body weight ratio of the model control group (MC) were significantly lower than that of the normal control group (P0.01). There were significant differences between the mean values (P0.01). Compared with NC, TCU TGG-LDL increased and decreased significantly (P0.01), compared with MC (1) (133mg kg-1267 mg kg-1800 mg kg-1), TCG-TG decreased significantly, and 133mg kg-1267 mg kg-1 had no significant change, but 800mg kg-1 decreased LDL-C (P0.05). At the same time, compared with NC, APNs of MC rats decreased significantly (P0.01) and those of MC-treated rats decreased. Western blot assay showed that compared with MC group, the expression of AdipoR2p38PGC-1PPAR 纬 protein in liver tissue of rats treated with MC-CBE (I) was higher than that of MC group. The expression of PPAR 纬 protein in adipose tissue was higher than that in muscle tissue. The expression of PPAR 纬 protein was not detected in adipose tissue. The results of RT-PCR showed that the expression of PPAR 纬 protein in rat liver was significantly decreased, while that of AdipoR2p38PPAR 纬 mRNA was significantly increased in rat liver. Conclusion: according to the evaluation method of weight-loss function in Annex 8 of "National Food and Drug Supervision, Baohua [2012] 107", the two kinds of extracts of coffee bean have the effect of reducing weight loss, and the mechanism may be to increase the level of serum APN and increase the expression of PGC-1 by increasing serum APN level, reducing the level of LP and increasing the expression of PGC-1. The level of PPAR- 纬 was upregulated to a certain extent, which promoted the adipose tissue to release APN, increased APN binding with Adipo R2 and activated the downstream AMPK 伪 p38 PPAR 伪, down-regulated SREBF1, thus promoted the decomposition of fat, inhibited the formation of fat, and achieved the effect of weight loss.
【学位授予单位】：浙江工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589.2

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