高尿酸血症细胞模型的构建及其在降尿酸肽筛选中的应用

发布时间：2018-07-06 08:00

本文选题：高尿酸血症 + 尿酸　；参考：《现代食品科技》2017年08期

【摘要】：高尿酸血症是由于嘌呤代谢紊乱使尿酸生成增加和(或)排泄减少所致的代谢性疾病,是引起痛风的重要生化基础。本文建立了一种高尿酸血症肝细胞模型,并研究了乳清蛋白肽的降尿酸活性。具体步骤为:将肝细胞铺板,分为空白对照组和模型组,48 h后,模型组给予一定浓度的腺苷溶液,空白对照组给予基础培养基,36 h后,加入黄嘌呤氧化酶继续孵育12 h,收集细胞上清液,高效液相测定尿酸含量。结果显示模型组尿酸含量显著高于空白对照组,且腺苷的最佳诱导浓度、细胞铺板密度及诱导时间分别为2.5 mmol/L、10~5个/m L、36 h。采用最佳建模条件,将非布索坦和乳清蛋白肽用于该模型,结果显示相对于模型组,非布索坦组尿酸含量显著降低,并呈一定的浓度剂量效应,该结果进一步表明高尿酸血症模型构建成功。采用乳清蛋白酶解液孵育肝细胞后,当乳清蛋白肽达到一定浓度时,相对于模型组其尿酸生成量显著降低,表明乳清蛋白酶解物具有一定的降尿酸活性。
[Abstract]:Hyperuricemia is a metabolic disease caused by increased uric acid production and / or decreased excretion due to purine metabolic disorder, which is an important biochemical basis for gout. A hepatocyte model of hyperuricemia was established and the antiuric acid activity of whey protein peptide was studied. The specific steps were as follows: the hepatocytes were divided into blank control group and model group after 48 hours, the model group was given a certain concentration of adenosine solution, and the blank control group was given basic culture medium for 36 h. Xanthine oxidase was added for 12 hours to collect cell supernatant and determine uric acid content in high performance liquid phase. The results showed that the uric acid content in the model group was significantly higher than that in the control group, and the optimal concentration of adenosine, the cell paving density and the induction time were 2.5 mmol 路L ~ (-1) ~ (10 ~ 5) / mL ~ (-1) for 36 h, respectively. The results showed that the uric acid content of non-buzotam and whey protein peptide groups was significantly decreased compared with the model group, and the concentration of uric acid in the non-buzotam group was in a dose-dependent manner. The results further indicated that the model of hyperuricemia was successfully constructed. After incubating hepatocytes with whey proteolysis solution, when the concentration of whey protein peptide reached a certain concentration, the uric acid production was significantly decreased compared with the model group, which indicated that whey protease hydrolysate had a certain uric acid reducing activity.
【作者单位】：华南理工大学食品科学与工程学院;无限极(中国)有限公司;
【基金】：国家自然科学基金项目(31671804) 广东省自然科学杰出青年基金项目(S2013050013954) 教育部新世纪人才支持计划项目(NCET-13-0213) “广东特支计划”科技青年拔尖人才项目(2014TQ01N645)
【分类号】：R589.7

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