Caveolin-1在甘精胰岛素改善2型糖尿病小鼠糖代谢及骨骼肌炎症中的作用

发布时间：2018-07-06 11:37

本文选题：Caveolin- + 骨骼肌　；参考：《实用医学杂志》2017年08期

【摘要】：目的:观察Caveolin-1下调的2型糖尿病小鼠接受甘精胰岛素治疗后糖代谢及骨骼肌炎症的改善情况。方法:C57BL/6J小鼠随机分为2组:对照组(NC组)和2型糖尿病模型组(T组)。T组采用高脂高糖饮食加小剂量链脲佐菌素腹腔注射方法成模。T组根据胰岛素治疗及尾静脉接受病毒注射情况再分为4组:无干预组(T2DM组),接受胰岛素治疗组(insulin组),注射sh RNA沉默Caveolin-1表达并胰岛素治疗组(LV-sh RNACav1组),注射阴性病毒并胰岛素治疗组(LV-GFP),治疗组均皮下注射甘精胰岛素3周,记录小鼠随机血糖及胰岛素剂量,取小鼠骨骼肌用Western blot检测Caveolin-1及TNF-α的表达。结果:与insulin组及LV-GFP组小鼠相比,LV-sh RNACav1组小鼠控制血糖需要的甘精胰岛素量最大(P0.05),骨骼肌中炎症因子TNF-α表达量最多(P0.05)。结论:Caveolin-1与机体胰岛素敏感性相关,并可能参与了胰岛素对骨骼肌炎症的改善作用。
[Abstract]:Aim: to observe the improvement of glucose metabolism and skeletal muscle inflammation in type 2 diabetic mice treated with insulin glargine. Methods Twenty C57BL / 6J mice were randomly divided into two groups: control group (NC group) and type 2 diabetic model group (T group). Subdivided into 4 groups: no intervention group (T2DM group), insulin treatment group (insulin group), injection of sh RNA silencing Caveolin-1 expression and insulin treatment group (LV-sh RNA Cav1 group), negative virus injection combined with insulin treatment group (LV-GFP), treatment group skin Insulin glargine was injected for 3 weeks. Random blood glucose and insulin were recorded and the expressions of Caveolin-1 and TNF- 伪 in skeletal muscle of mice were detected by Western blot. Results: compared with insulin group and LV-GFP group, LV-sh RNA Cav1 group had the largest amount of insulin glargine (P0.05) and the highest expression of TNF- 伪 in skeletal muscle (P0.05). ConclusionCaveolin-1 is associated with insulin sensitivity and may be involved in the improvement of skeletal muscle inflammation by insulin.
【作者单位】：中山大学附属第三医院内分泌与代谢病广东省糖尿病重点实验室;
【基金】：国家自然基金(编号:81070661) 诺和诺德中国糖尿病英才研究基金(2014)
【分类号】：R587.1

【参考文献】