游离SIVmac239病毒与T细胞介导两种方式感染内皮细胞的效果比较

发布时间：2018-07-06 15:48

本文选题：SIV + 艾滋病脑病　；参考：《中国比较医学杂志》2016年06期

【摘要】：目的比较游离病毒感染与T细胞介导的感染方式对SIV感染内皮细胞的影响,探索SIVmac239感染内皮细胞的主要途径,从而为SIV入侵血脑屏障的机制提供理论基础。方法采用游离SIV病毒直接感染内皮细胞和SIV感染的CEMx174细胞与内皮细胞共培养的两种方式,通过巢式PCR、间接免疫荧光法、Western blotting以及ELISA检测内皮细胞的感染程度。结果两种感染方式都能在内皮细胞内检测到前病毒DNA,感染细胞与内皮细胞共培养时,内皮细胞内前病毒DNA含量,SIV P27蛋白表达量和细胞培养上清液中P27含量远高于游离病毒直接感染的方式。结论细胞介导的感染方式相较于游离病毒直接感染方式对内皮细胞的感染能力更强,可能是SIV病毒入侵血脑屏障的主要途径。
[Abstract]:Objective to compare the effects of free virus infection and T cell mediated infection on SIV infected endothelial cells, and to explore the main pathway of SIVmac239 infection on endothelial cells, so as to provide a theoretical basis for the mechanism of SIV invading the blood-brain barrier. Methods the infection degree of endothelial cells was detected by nested PCR, indirect immunofluorescence assay and Elisa, respectively, in which free SIV virus directly infected endothelial cells and CEMx174 cells infected with SIV-infected CEMx174 cells co-cultured with endothelial cells. Results Previrus DNA could be detected in endothelial cells in both ways, and when the infected cells were co-cultured with endothelial cells, The expression of SIV P27 protein and the P27 content in the supernatant of cell culture were significantly higher than those in the direct infection of free virus. Conclusion compared with the direct infection of free virus, the infection mode mediated by SIV has stronger ability to infect endothelial cells, which may be the main way for SIV virus to invade the blood-brain barrier.
【作者单位】：北京协和医学院比较医学中心中国医学科学院医学实验动物研究所卫生部人类疾病比较医学重点实验室国家中医药管理局人类疾病动物模型三级实验室;
【基金】：国家十二五科技重大专项课题(2012ZX10004-501-001和2013ZX10004-608-003)
【分类号】：R512.91

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