滤泡辅助性T细胞及白介素-21在类风湿关节炎外周血和关节滑液中的表达及临床意义
发布时间：2018-07-13 19:35
【摘要】：目的:本实验拟采用流式细胞术(FCM)和酶联免疫吸附(ELISA)的方法分别检测类风湿关节炎(RA)患者外周血单个核细胞(PBMC)和关节液中CD4+CXCR5+T细胞的表达以及相关细胞因子白细胞介素(IL)-21的表达,探讨滤泡辅助性T细胞(Tfh)在类风湿关节炎发病机制中的作用。方法:采集RA病人和对照组(骨关节炎患者)外周血、关节液,流式细胞术检测外周血及关节液中CD4+CXCR5+T细胞的百分比;血清及关节液中IL-21蛋白的水平用酶联免疫吸附法(ELISA法)检测。结果:RA组活动期及缓解期外周血单个核细胞中Tfh细胞百分率(14.15%±2.74%,12.80%±2.15%)均明显高于对照组(7.26%±1.62%)(P0.05),活动期与缓解期组间比较,差异无统计学意义;RA组活动期及缓解期IL-21水平(41.88±15.63pg/ml,37.27±11.44pg/ml)均明显高于对照组(22.25±1.18)pg/ml(P0.05),RA组中活动期与缓解期组间比较,差异无统计学意义;RA组活动期关节液Tfh细胞阳性表达率(3.15%±1.42%)与对照组关节液Tfh细胞阳性表达率(2.88%±1.17%)相比,差异无统计学意义;RA组活动期关节液中IL-21蛋白水平(37.92±15.49)pg/ml与对照组关节液IL-21蛋白水平(34.31±5.15)pg/ml相比,差异无统计学意义。RA患者外周血中Tfh细胞百分比、IL-21水平与抗环瓜氨酸肽抗体(An-CCP)、类风湿因子(RF)均呈正相关结论:Tfh细胞可能启动了RA的发病过程,但对疾病活动未见明显促进作用;IL-21可能并非为启动RA疾病活动的主要因素。
[Abstract]:Objective: flow cytometry (FCM) and enzyme-linked immunosorbent assay (Elisa) were used to detect the expression of CD4 CXCR5 T cells and cytokines in peripheral blood mononuclear cells (PBMC) and joint fluid of patients with rheumatoid arthritis (RA). Expression of interleukin (IL) -21, To investigate the role of follicular helper T cells (Tfh) in the pathogenesis of rheumatoid arthritis. Methods: peripheral blood and joint fluid were collected from RA patients and controls (osteoarthritis patients). The percentage of CD4 CXCR5 T cells in peripheral blood and joint fluid were detected by flow cytometry. The level of IL-21 protein in serum and joint fluid was detected by enzyme linked immunosorbent assay (Elisa). Results the percentage of Tfh cells in the peripheral blood mononuclear cells in the active phase and remission phase of the control group (14.15% 卤2.74 卤12.80% 卤2.15%) was significantly higher than that in the control group (7.26% 卤1.62%) (P0.05). The level of IL-21 in RA group (41.88 卤15.63pg / ml, 37.27 卤11.44pg/ml) was significantly higher than that in control group (22.25 卤1.18) pg/ml (P0.05). The positive expression rate of Tfh cells in active synovial fluid (3.15% 卤1.42%) in RA group was higher than that in control group (2.88% 卤1.17%). The level of IL-21 protein in active synovial fluid of RA group was (37.92 卤15.49) pg/ml and that in control group was (34.31 卤5.15) pg/ml. There was no significant difference in the percentage of Tfh cells in peripheral blood of RA patients. The level of IL-21 was positively correlated with anti-cyclic citrullin-peptide antibody (An-CCP) and rheumatoid factor (RF). But there was no obvious effect on disease activity. IL-21 may not be the main factor to initiate RA disease activity.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R593.22
[Abstract]:Objective: flow cytometry (FCM) and enzyme-linked immunosorbent assay (Elisa) were used to detect the expression of CD4 CXCR5 T cells and cytokines in peripheral blood mononuclear cells (PBMC) and joint fluid of patients with rheumatoid arthritis (RA). Expression of interleukin (IL) -21, To investigate the role of follicular helper T cells (Tfh) in the pathogenesis of rheumatoid arthritis. Methods: peripheral blood and joint fluid were collected from RA patients and controls (osteoarthritis patients). The percentage of CD4 CXCR5 T cells in peripheral blood and joint fluid were detected by flow cytometry. The level of IL-21 protein in serum and joint fluid was detected by enzyme linked immunosorbent assay (Elisa). Results the percentage of Tfh cells in the peripheral blood mononuclear cells in the active phase and remission phase of the control group (14.15% 卤2.74 卤12.80% 卤2.15%) was significantly higher than that in the control group (7.26% 卤1.62%) (P0.05). The level of IL-21 in RA group (41.88 卤15.63pg / ml, 37.27 卤11.44pg/ml) was significantly higher than that in control group (22.25 卤1.18) pg/ml (P0.05). The positive expression rate of Tfh cells in active synovial fluid (3.15% 卤1.42%) in RA group was higher than that in control group (2.88% 卤1.17%). The level of IL-21 protein in active synovial fluid of RA group was (37.92 卤15.49) pg/ml and that in control group was (34.31 卤5.15) pg/ml. There was no significant difference in the percentage of Tfh cells in peripheral blood of RA patients. The level of IL-21 was positively correlated with anti-cyclic citrullin-peptide antibody (An-CCP) and rheumatoid factor (RF). But there was no obvious effect on disease activity. IL-21 may not be the main factor to initiate RA disease activity.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R593.22
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