二甲双胍对正常饮食大鼠血清成纤维细胞生长因子19、21及胰腺成纤维细胞生长因子受体1的影响

发布时间：2018-07-17 05:15

【摘要】：目的探讨二甲双胍(METF)对血清成纤维细胞生长因子(FGF)19和FGF21及胰腺成纤维细胞生长因子受体(FGFR)1和Akt通路上分子的影响。方法给予8周龄健康雄性Wistar大鼠(40只)标准饮食,随机分为正常对照(Con)组和METF(500 mg·kg~(-1)·d~(-1))。在24 w末检测血清生化学指标及FGF19和FGF21水平。蛋白免疫印迹检测胰腺组织FGFR1,Akt、磷酸化的Akt(p-Akt),FoxO1、磷酸化的FoxO1(p-FoxO1)的表达水平。结果 METF干预可以减轻大鼠的体重,降低大鼠空腹血糖、低密度脂蛋白和胆汁酸水平。METF组大鼠血清FGF21较Con组明显升高(P0.05),而FGF19较Con组明显减低(P0.05)。此外,胰腺FGFR1的表达水平及Akt及FoxO1的磷酸化水平在METF干预后明显升高(均P0.05)。结论 METF可以降低血清FGF19、升高血清FGF21水平,对两者产生相反的作用。此外,METF可能通过增加FGFR1的表达、Akt及FoxO1的磷酸化对胰腺功能产生影响。
[Abstract]:Objective to investigate the effects of metformin (METF) on serum fibroblast growth factor (FGF19), FGF21, pancreatic fibroblast growth factor receptor (FGFR) 1 and molecules in Akt pathway. Methods Forty healthy male Wistar rats aged 8 weeks were randomly divided into normal control group (Con) and METF (500mg kg ~ (-1) d ~ (-1). Serum biochemical indexes, FGF19 and FGF21 levels were detected at the end of 24 weeks. The expression of FGFR1 Akt, phosphorylated Akt (p-Akt) and phosphorylated FoxO1 (p-FoxO1) were detected by Western blot. Results METF could reduce the weight of rats, decrease fasting blood glucose, low density lipoprotein and bile acid levels. The serum FGF21 of METF group was significantly higher than that of Con group (P0.05), while FGF19 was significantly lower than that of Con group (P0.05). In addition, the expression of FGFR1 and phosphorylation of Akt and FoxO1 in pancreas increased significantly after METF intervention (P0.05). Conclusion METF can decrease serum FGF19 and increase serum FGF21 level, which has the opposite effect on both. In addition, METF may affect pancreatic function by increasing the expression of FGFR1 and phosphorylation of Akt and FoxO1.
【作者单位】：山东大学医学院;山东大学附属济南市中心医院内分泌科;泰山医学院;
【基金】：国家自然科学基金(81170771) 山东省科技发展计划(2012GSF11803) 山东省重点研发项目(2016GSF201019) 济南市国际合作项目(201011008)
【分类号】：R587.1

【参考文献】