2型糖尿病患者血清锌离子水平与氧化应激及内皮损伤的研究

发布时间：2018-07-18 16:01

【摘要】：背景：糖尿病（diabetes mellitus，DM）是由遗传和环境因子共同作用而引起的以胰岛素及β细胞功能障碍致糖代谢紊乱为主要表现的临床综合征。近年研究显示，糖尿病与氧化应激（oxidative stress,OS）有密切联系。氧化应激是指机体在遭受各种有害刺激，生成体内高活性分子，如活性氧自由基和活性氮自由基的产生过多，氧化程度超出氧化物的清除，氧化系统和抗氧化系统失衡，导致组织损伤。丙二醛（malondialdehyde,MDA）是氧化应激的指标，而超氧化物岐化酶superoxide dismutase,SOD）则是抗氧化系统的指标。研究认为内皮细胞功能紊乱是糖尿病心血管并发症的始动因素。血管内皮细胞分泌的多种活性因子一氧化氮（nitric oxide,NO）和内皮素（endothelin,ET）可导致内皮功能障碍。锌离子参与胰岛素的合成、分泌、生物活性和抗原性，锌与糖尿病的发生、发展及并发症的发生都密切相关。同时，锌是维持细胞正常功能的必需金属元素，锌可作为多种酶和蛋白质的辅助因子参与抗氧化作用。因此，本研究通过探讨锌离子对2型糖尿病患者氧化应激的影响及对2型糖尿病患者血清内皮功能的影响，寻找干预靶点进行有效的预防及治疗。目的：本实验通过检测对照组及糖尿病组血清氧化应激指标（SOD、MDA）及内皮损伤因子(NO、ET)的含量水平，探讨锌离子与氧化应激及内皮损伤因子的关系，明确锌对糖尿病患者心血管并发症的保护作用。方法：吉大一院老年病科就诊2型糖尿病患者为糖尿病组，同时来自医院就诊的非糖尿病患者为对照组，研究对象的基本资料、调查患者情况、近期用药情况。检测所有研究对象血清中锌离子水平。采用试剂盒测定研究对象血清中NO、ET及SOD、MDA。分析研究对象血清中锌离子水平，，研究对象血清中NO、ET、MDA、SOD与锌离子水平的相关性。结果： 1、糖尿病组患者血清锌离子水平明显低于对照组血清锌离子水平，有显著统计学差异（P0.05）。 2、糖尿病组与对照组相比患者抗氧化指标SOD明显下降，氧化应激指标MDA明显升高，有显著统计学差异（P0.05）。 3、糖尿病组患者与对照组相比内皮损伤因子NO、ET水平升高，有统计学意义（P0.05）。结论： 1、2型糖尿病患者血清离子发生紊乱，其中锌离子、镁离子在糖尿病组患者血清中明显下降。 2、2型糖尿病患者抗氧化能力明显下降，同时氧化损伤明显增加。 3、2型糖尿病组患者内皮功能明显受损。
[Abstract]:Background: diabetes mellitus (diabetes mellitus DM) is a clinical syndrome caused by the interaction of genetic and environmental factors, which is mainly characterized by impaired glucose metabolism caused by insulin and 尾 cell dysfunction. Recent studies have shown that diabetes is closely associated with oxidative stress. Oxidative stress refers to the excessive production of highly active molecules such as reactive oxygen free radicals and active nitrogen free radicals in the body under various harmful stimuli, the degree of oxidation exceeding the removal of oxides, and the imbalance between the oxidation system and the antioxidant system. Causing tissue damage. Malondialdehyde (malondialdehyde) is an index of oxidative stress, while superoxide dismutase (superoxide) is an index of antioxidant system. It is believed that endothelial cell dysfunction is the initiator of cardiovascular complications in diabetes mellitus. Endothelial dysfunction can be caused by nitric oxide (no) and endothelin et (et) secreted by vascular endothelial cells. Zinc ion is involved in the synthesis, secretion, bioactivity and antigenicity of insulin. Zinc is closely related to the occurrence, development and complications of diabetes mellitus. At the same time, zinc is an essential metal element to maintain the normal function of cells. Zinc can be used as an auxiliary factor of many enzymes and proteins to participate in antioxidation. Therefore, in this study, the effect of zinc ion on oxidative stress and serum endothelial function in type 2 diabetes mellitus patients was investigated to find effective intervention targets for prevention and treatment. Objective: to investigate the relationship between zinc ion and oxidative stress and endothelial injury factor by detecting the levels of serum oxidative stress index (SOD- MDA) and endothelial injury factor (NO-et) in control group and diabetic group. To determine the protective effect of zinc on cardiovascular complications in diabetic patients. Methods: type 2 diabetic patients in the geriatric department of Jida first Hospital were treated as diabetic group and non-diabetic patients in hospital as control group. The basic data of the subjects were investigated and the recent drug use was investigated. Serum zinc ion levels were measured in all subjects. The determination of NONET and SODX MDAs in serum was carried out by using the kit. The level of zinc ion in serum and the correlation between SOD and zinc ion level in serum were analyzed. Results: 1. The serum zinc ion level in the diabetic group was significantly lower than that in the control group (P0.05). Oxidative stress index MDA significantly increased, there was significant difference (P0.05). 3, compared with the control group, the level of endothelium injury factor NON-ET in diabetic patients increased significantly (P0.05). Conclusion: (1) the serum ions of type 2 diabetes mellitus patients have disorder, among which zinc ion and magnesium ion are obviously decreased in the serum of the patients with type 2 diabetes mellitus, and the antioxidant ability of type 2 diabetes mellitus patients is obviously decreased. At the same time, oxidative injury was significantly increased. Endothelial function was significantly impaired in type 3 diabetes mellitus patients.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.2

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