糖基化终末产物损伤冠状动脉平滑肌细胞K_v通道

发布时间：2018-07-26 06:18

【摘要】：目的研究糖基化终末产物(advanced glycation end products,AGEs)对大鼠冠状动脉平滑肌细胞电压门控性钾离子(voltage-gated K~+,K_v)通道电流的影响。方法分离大鼠冠状动脉平滑肌细胞,分为空白对照组、糖基化牛血清白蛋白(AGEbull serum albumin,AGE-BSA)组、AGE-BSA+抗AGE受体抗体(anti-receptor of AGEs immunoglobulin G,anti-RAGE Ig G)组进行干预。采用膜片钳技术检测各组细胞K_v通道电流,采用Western blotting、实时荧光定量PCR方法检测各组细胞K_v1.2和K_v1.5通道蛋白及mRNA的表达。结果 AGE-BSA直接干预冠状动脉平滑肌细胞明显抑制了平滑肌细胞的K_v电流达32.7%,并使K_v1.2和K_v1.5通道蛋白及mRNA的表达明显下调。而给予anti-RAGE Ig G预处理30 min后再加入AGE-BSA刺激,平滑肌细胞的K_v电流密度及K_v1.2和K_v1.5通道蛋白及mRNA的表达与空白对照组相比,差异无统计学意义。结论 AGEs通过结合RAGE损伤冠状动脉平滑肌细胞K_v通道。
[Abstract]:Aim to study the effect of glycation end product (advanced glycation end products ages on the current of voltage-gated K ~ + K _ v channel in rat coronary artery smooth muscle cells. Methods Rat coronary artery smooth muscle cells were isolated and divided into two groups: the control group, and the glycosylated bovine serum albumin (BSA) (AGEbull serum albumin AGE-BSA group and the anti-receptor of AGEs immunoglobulin GG anti-RAGE Ig G) group. The expression of K_v1.2, K_v1.5 channel protein and mRNA were detected by patch clamp technique, Western blotting and real-time fluorescence quantitative PCR. Results AGE-BSA directly inhibited the KV current of smooth muscle cells and significantly down-regulated the expression of K_v1.2 and K_v1.5 channel protein and mRNA. The KV current density, the expression of K_v1.2, K_v1.5 channel protein and mRNA in smooth muscle cells after 30 min pretreatment with anti-RAGE Ig G were not significantly different from those in the control group. Conclusion AGEs can injure K _ V channel of coronary artery smooth muscle cells by combining RAGE.
【作者单位】：首都医科大学附属北京友谊医院心血管中心;首都医科大学基础医学院生理学与病理生理学系;
【基金】：国家自然科学基金(30971240) 北京市自然科学基金(7122053) 北京市卫生系统高层次卫生技术人才资助项目(2013-3-060)~~
【分类号】：R543;R587.2

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