慢性高原病患者血清GC-TOF-MS代谢组学的初步研究

发布时间：2018-07-31 08:39

【摘要】：目的:研究慢性高原病患者气相色谱飞行时间质谱(GC-TOF-MS)代谢组学的变化,并鉴定血清代谢图谱的内源性代谢物,探讨青海玉树世居藏族居民潜在的差异性代谢标志物。方法:收集10例青海玉树世居藏族慢性高原病患者(CMS组)和10例世居高原健康藏族居民(control组)血清,利用GC-TOF-MS代谢组学和多元回归分析方法,如主成分分析法(PCA)、偏最小二乘法判别分析(PLS-DA)和正交偏最小二乘法判别分析(OPLS-DA)模式识别分析各代谢物的变化。结果:PCA、PLS-DA和OPLS-DA模式判别分析结果显示,两组血清代谢组均表现出明显聚类型分布。CMS组血清中延胡索酸、肌苷、植烷酸、2,2-二甲基丁二酸、β-酮己二酸、异亮氨酸、甘氨酸、丝氨酸、L-半胱氨酸、瓜氨酸、三甲基赖氨酸、1-磷酸葡萄糖和核糖水平显著高于control组;CMS组血清中下降的代谢物有硫酸、草酸、谷氨酰胺和来苏糖。结论:慢性高原病患者和高原健康藏族居民血清代谢物水平有显著性差异,这些血清代谢物的改变可能参与了慢性高原病的发生与发展。
[Abstract]:Objective: to study the changes of gas chromatography time of flight mass spectrometry (GC-TOF-MS) metabolism in patients with chronic altitude disease, identify the endogenous metabolites of serum metabolic map, and explore the potential differential metabolic markers of Tibetan residents living in Yushu, Qinghai. Methods: the serum samples of 10 Tibetan patients with chronic altitude disease (CMS group) and 10 healthy Tibetan residents (control group) were collected and analyzed by GC-TOF-MS metabolomics and multivariate regression analysis. For example, principal component analysis (PCA), (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) are used to analyze the changes of metabolites. Results the results of discriminant analysis of the two groups showed that the concentration of maleic acid, inosine, phytinic acid, 尾 -ketoadipic acid, isoleucine, glycine in the serum of the two groups were significantly different from that of the control group, and the results of discriminant analysis of the two groups showed that the distribution of maleic acid, inosine, phytinic acid, 尾 -ketoadipic acid, isoleucine and glycine were significant. The serum levels of L-cysteine, citrullinic acid, trimethyllysine 1-phosphate glucose and ribose were significantly higher than those of control group in which the decreased metabolites were sulfuric acid, oxalic acid, glutamine and lethose. Conclusion: there are significant differences in serum metabolites between the patients with chronic altitude disease and the healthy Tibetan residents at high altitude. The changes of these serum metabolites may be involved in the occurrence and development of chronic high altitude disease.
【作者单位】：青海大学医学院高原医学研究中心;
【基金】：国家重点基础研究发展计划(973计划)项目(No.2012CB518200) 国家自然科学基金资助项目(No.31571231) 青海-犹他联合重点实验室项目(No.2014-ZJ-Y39) 青海大学医学院中青年科研基金资助项目(No.2015-KY-2)
【分类号】：R594.3

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