富半胱氨酸蛋白61在高脂诱导肥胖小鼠脂肪组织中的表达

发布时间：2018-08-13 17:45

【摘要】：目的探讨富半胱氨酸蛋白61(Cyr61)在肥胖小鼠脂肪组织中的表达、分布及其与脂肪慢性炎症的关系。方法 4周龄雄性C57BL/6小鼠30只,随机分为普通饮食组(NC组)和高脂喂养组(HFD组),每组15只。HFD组给予60%脂肪含量高脂饲料喂养,构建肥胖小鼠模型,NC组给予普通饲料喂养。造模成功后测量小鼠体脂含量,通过在腹腔注射葡萄糖进行葡萄糖耐量试验(IPGTT)检测糖耐量和血脂,取睾周脂肪检测Cyr61的表达及分布,并检测脂肪组织肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的mRNA表达量。结果 HFD组脂质量、体内脂肪含量明显增加,糖耐量减低,血清总胆固醇和低密度脂蛋白胆固醇较正常高(P0.05)。HFD组脂肪组织TNF-α、IL-6表达上调(P0.05),Cyr61在HFD组脂肪组织中蛋白及mRNA水平均增加(P0.05),且主要分布于脂肪组织基质中。结论 Cyr61在肥胖小鼠脂肪组织表达明显增加,主要定位于脂肪组织基质中,且与炎症因子表达具有一致性,提示其可能与慢性炎症的发生相关。
[Abstract]:Objective to investigate the expression and distribution of cysteine rich protein 61 (Cyr61) in adipose tissue of obese mice and its relationship with chronic fat inflammation. Methods Thirty four-week-old male C57BL/6 mice were randomly divided into normal diet group (NC group) and high-fat feeding group (HFD group). 15 rats in each group were fed with 60% fat high-fat diet. After the establishment of the model, the body fat content of mice was measured. Glucose tolerance test (IPGTT) was performed by intraperitoneal glucose tolerance test (GTT), and serum lipid and glucose tolerance (GTT) were measured by (IPGTT), and the expression and distribution of Cyr61 were detected by pericorchidism fat. The mRNA expression of tumor necrosis factor- 伪 (TNF- 伪) and interleukin-6 (IL-6) in adipose tissue was detected. Results in HFD group, the fat mass, body fat content and glucose tolerance were significantly increased. Serum total cholesterol and low density lipoprotein cholesterol (LDL-C) were higher than normal (P0.05). The expression of TNF- 伪 and IL-6 in adipose tissue of HFD group was up-regulated (P0.05). The levels of protein and mRNA in adipose tissue of HFD group were increased (P0.05), and mainly distributed in adipose tissue matrix. Conclusion the expression of Cyr61 in adipose tissue of obese mice is significantly increased, mainly located in adipose tissue matrix and consistent with the expression of inflammatory factors, suggesting that Cyr61 may be related to the occurrence of chronic inflammation.
【作者单位】：山东大学齐鲁医院内分泌科;
【基金】：国家自然科学基金(81370943)
【分类号】：R589.2

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