番茄红素及维生素E对燃煤型氟中毒致大鼠睾丸组织中Clusterin蛋白表达的影响及机制

发布时间：2018-08-14 17:55

【摘要】：目的研究番茄红素及维生素E对燃煤型氟中毒致大鼠睾丸组织中Clusterin蛋白表达的影响及机制。方法 60只雄性SD大鼠分为低、中、氋氟组、氋氟+维生素E组、氋氟+番茄红素组和对照组,每组10只。染毒组喂饲含不同比例的燃煤型氟中毒病区煤烘玉米的饲料,构建燃煤型氟中毒动物模型,氋氟+维生素E组和氋氟+番茄红素组分别喂饲添加维生素E和番茄红素的病区煤烘玉米配制饲料,对照组给予正常饲料。观察各组大鼠氟斑牙情况;氟离子选择电极法检测大鼠尿和睾丸组织氟含量;HE染色光镜下观察睾丸组织病理学改变;原位缺口末端标记(TUNEL)染色观察生精细胞凋亡情况;免疫组化法检测大鼠睾丸组织中Clusterin蛋白表达。结果与对照组相比,低、中、高氟组大鼠氟斑牙严重程度、尿氟及睾丸组织氟含量、睾丸组织病理学损伤程度和睾丸组织生精细胞凋亡指数均依次增加(P0.05),各氟剂量组上述指标随氟剂量的增大而增大;与高氟组相比,氋氟+维生素E组和氋氟+番茄红素组上述指标明显降低;与对照组相比,低、中、高氟组大鼠Clusterin蛋白水平依次下降(P0.05),各氟剂量组Clusterin蛋白表达水平随氟剂量的增大而下降。与高氟组相比,氋氟+维生素E组和氋氟+番茄红素组Clusterin蛋白水平明显升高。结论燃煤型氟中毒可引起大鼠睾丸组织的性生殖损害,生精细胞凋亡率增加,Clusterin蛋白的表达降低。抗氧化剂可显著降低生殖细胞凋亡率,提高Clusterin蛋白的表达,改善雄性生殖功能的损伤。
[Abstract]:Objective To study the effect of lycopene and vitamin E on the expression of Clusterin protein in the testis of rats with coal-burning fluorosis and its mechanism.Methods Sixty male SD rats were divided into low, medium, fluoride group, fluoride + vitamin E group, fluoride + lycopene group and control group, with 10 rats in each group. The animal model of coal-burning fluorosis was established by using coal-baked corn as feed. The rats were fed with coal-baked corn supplemented with vitamin E and lycopene respectively, and the rats in control group were fed with normal diet. Fluoride content; HE staining light microscopic observation of testicular histopathological changes; in situ notch end labeling (TUNEL) staining observation of spermatogenic cell apoptosis; immunohistochemical detection of Clusterin protein expression in rat testicular tissue. The degree of histopathological damage and the apoptosis index of spermatogenic cells in testicular tissue increased in turn (P 0.05). The above indexes increased with the increase of fluoride dosage in each fluoride dosage group. Compared with the high fluoride group, the above indexes in the fluoride + vitamin E group and the fluoride + lycopene group decreased significantly; Compared with the control group, the Clusterin protein water in the low, middle and high fluoride groups was lower. Compared with the high fluoride group, the levels of Clusterin protein in the fluoride + vitamin E group and the fluoride + lycopene group were significantly higher. Conclusion Coal-burning fluorosis can cause sexual reproductive damage in rat testis, and the apoptosis rate of spermatogenic cells is increased. Antioxidants can significantly reduce the apoptosis rate of germ cells, increase the expression of Clusterin protein and improve the damage of male reproductive function.
【作者单位】：贵州医科大学附属医院泌尿外科;
【基金】：国家自然科学基金项目(81360097) 贵州省科技厅项目黔科合LH字[2014]7126 贵州省卫计委科技基金项目(gzwjkj2014-1-035);贵州省卫计委科技基金项目(gzwjkj2015-1-036) 贵州省高层次人才基金(NO:TZJF—2011年—27号)科技厅国际合作项目(黔科合外G字2012-7015)
【分类号】：R599.1

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