中国MSM人群中HIV-1亚型与不同位点耐药突变发生频率的Meta分析

发布时间：2018-08-18 21:42

【摘要】：目的了解我国男男性行为(men who have sex with men,MSM)人群中人类获得性免疫缺陷病毒1型(human immunodeficiency virus type 1,HIV-1)亚型与不同耐药位点突变发生频率的情况。方法检索CNKI、CBM、万方、Pub Med、Web of science、Embase等数据库,收集有关中国MSM人群HIV-1亚型与耐药基因突变的现况调查研究,采用系统评价的方法严格筛选文献,以美国医疗保健研究与质量局(the agency for healthcare research and quality,AHRQ)质量评价条目评价文献质量,对所提取数据采用Freeman-Tukey双反正弦变换法计算各位点合并的耐药突变发生频率,以不同亚型为分组进行亚组分析,采用Begg’s test估计发表偏倚,统计分析由Stata 12.0软件完成。结果共纳入10篇研究文献,获得耐药突变位点信息23个,包含亚型五种(01AE、07BC、08BC、B、URFs)。突变发生频率超过5%的位点有:A71T/V/I 17.42%(8.20~29.17),V179D/E 11.06%(6.24~17.00),Y181C/N11.95%(0.50~35.28),L10I/V 7.06%(4.14~10.65),K103N 5.67%(1.75~11.66)。M46I/L、T69D/NS、L74I/S与K103N/R的突变仅在01AE亚型中检测到,B亚型检测出发生突变的位点其突变发生频率均高于其他亚型。结论我国MSM感染者中01AE亚型携带更多种类的耐药突变相关位点,B亚型耐药相关位点的突变频率更高,上述两种亚型及其耐药突变对传播耐药发生率的贡献度大,需要加强防治。
[Abstract]:Objective to investigate the frequency of human acquired immunodeficiency virus type 1 (human immunodeficiency virus type 1) HIV-1) subtypes and different drug resistance loci in men who had sex with men in China. Methods the database of HIV-1 subtype and drug resistance gene mutation in Chinese MSM population were searched, and the literature was screened by systematic evaluation. The literature quality was evaluated by the quality evaluation item of (the agency for healthcare research and quality Agency of USA. The frequency of drug resistance mutation was calculated by Freeman-Tukey double inverse sinusoidal transform method. The subgroup analysis was carried out in different subtypes, and the publication bias was estimated by Begg's test. The statistical analysis was done by Stata 12.0 software. Results A total of 10 studies were carried out and 23 information on drug resistance mutation sites were obtained, including 5 subtypes (01AEO07BCC08BCCnBUFs). The frequency of mutation more than 5% was: 1 / A 71T / V / V / I 17.42% (8.20 / 29.17) V179DE 11.06% (6.240.00) Y181C / N11.95% (0.505.28) L10IV 7.06% (4.1410.65) K103N 5.67% (1.751.66) .M46IP / LT69Dr / 74IS and K103N/R only detected in 01AE subtype, the frequency of mutation in B subtype was higher than that in other subtypes. Conclusion the mutation frequency of 01AE subtype in MSM infected patients is higher than that of 01AE subtype B subtype related to drug resistance. The two subtypes and their mutations contribute greatly to the incidence of transmission resistance, so it is necessary to strengthen prevention and treatment.
【作者单位】：东南大学公共卫生学院流行病学与卫生统计学系流行病学教研室;
【基金】：高等学校博士学科点专项科研基金(20130092110048)
【分类号】：R512.91

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