富氢生理盐水对大鼠痛风性关节炎的保护作用

发布时间：2018-08-20 19:24

【摘要】：目的探讨富氢生理盐水对大鼠痛风性关节炎(GA)的保护作用。方法雄性Wistar大鼠40只,随机分为4组:正常对照组、模型组、秋水仙碱组、富氢盐水组,富氢盐水组予10 mL/kg富氢盐水,秋水仙碱组予0.3 mg/kg秋水仙碱,正常对照组、模型组给予同体积的吐温-生理盐水,各组均灌胃给药2次/d,连续给药4周,在最后1次灌胃后采用尿酸钠右踝关节腔注射法进行大鼠GA的造模。于造模前后分别测量大鼠受试关节周径,白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的含量,并取受试关节软组织行病理检查,结合大鼠的行为学表现对治疗效果进行评价。结果与模型组比较,富氢盐水组、秋水仙碱组大鼠步态有明显改善(P0.05),受试关节周径明显缩小(P0.01),血清中IL-1β、TNF-α表达水平显著降低(P0.01),右踝关节滑膜炎症反应显著降低。结论富氢生理盐水能减轻GA大鼠炎症反应,对大鼠GA具有一定的疗效。
[Abstract]:Objective to investigate the protective effect of hydrogen rich saline on (GA) in rats with gouty arthritis. Methods 40 male Wistar rats were randomly divided into 4 groups: normal control group, model group, colchicine group, hydrogen enriched saline group (10 mL/kg), colchicine group (0.3 mg/kg) and normal control group. The model group was given the same volume of Tween-normal saline. The rats in each group were given intragastric administration for 2 times / d for 4 weeks. After the last gavage, the rat model of GA was established by injection of sodium uric acid into the right ankle joint. The peripheral diameter, interleukin-1 尾 (IL-1 尾) and tumor necrosis factor- 伪 (TNF- 伪) were measured before and after the establishment of the model. Results compared with the model group, the gait, circumference, IL-1 尾 -TNF- 伪 expression and synovial inflammation of the right ankle were significantly decreased in the hydrogen-rich saline group and colchicine group (P 0.05), the peripheral diameter of the joint was significantly decreased (P0.01), and the expression of IL-1 尾 -TNF- 伪 in serum was significantly decreased (P0.01). Conclusion Hydrogen-rich saline can alleviate the inflammatory reaction in GA rats and has a certain therapeutic effect on GA in rats.
【作者单位】：河池市人民医院骨科;桂林医学院附属医院骨科;
【分类号】：R589.7

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