血红素氧合酶系统对一氧化碳中毒后体外培养少突胶质细胞Nogo-A的影响

发布时间：2018-08-21 12:58

【摘要】：背景:鉴于Nogo-A蛋白及其受体Ng R分别表达于中枢神经系统的少突胶质细胞与神经元,而一氧化碳中毒迟发性脑病患者颅脑影像学上白质脱髓鞘突出,推测Nogo-A蛋白及NgR系统参与急性一氧化碳中毒脑损害,可能与一氧化碳中毒迟发性脑病关系密切。内源性一氧化碳(CO)是机体重要的气体信使分子之一,主要是由血红素氧合酶代谢产生的,其对Nogo-A体系的影响目前亦未见报道。目的:采用外源性CO处理体外培养少突胶质细胞,并用锌原卟啉9抑制血红素氧合酶系统活性,观察少突胶质细胞Nogo-A在mR NA及蛋白质水平的表达情况。方法:将体外培养的少突胶质细胞分为对照组、CO组和锌原卟啉9组。CO组和锌原卟啉9组细胞置于含体积分数1%CO的密封室内培养。锌原卟啉9组细胞在CO处理前,预先在培养液中加入10μmol/L锌原卟啉9。首先比较培养6,24和48 h时细胞中Nogo-A mR NA和蛋白的表达水平,检测表达水平最高时CO组和锌原卟啉9组细胞中Nogo-A mR NA和蛋白表达水平的差异。结果与结论:CO组细胞中Nogo-A mR NA和蛋白的表达水平均高于对照组,且都在培养24 h时表达水平最高。培养24 h时锌原卟啉9组细胞中Nogo-A m RNA和蛋白表达水平均高于CO组。提示排除在体情况中一氧化碳中毒所致的缺氧的影响,单纯外源性CO可诱导体外培养少突胶质细胞Nogo-A m RNA及蛋白表达水平增加;血红素氧合酶系统可抑制Nogo-A m RNA及蛋白的表达水平。
[Abstract]:BACKGROUND: Nogo-A protein and its receptor NgR are expressed in oligodendrocytes and neurons of the central nervous system respectively, and the white matter demyelinates prominently in the brain imaging of patients with delayed encephalopathy induced by carbon monoxide poisoning. It is speculated that Nogo-A protein and NgR system may be involved in the brain damage induced by acute carbon monoxide poisoning and may be associated with delayed brain injury induced by carbon monoxide poisoning. Endogenous carbon monoxide (CO) is one of the most important gaseous messenger molecules in the body. It is mainly produced by the metabolism of heme oxygenase. Its effect on Nogo-A system has not been reported yet. Objective: To culture oligodendrocytes in vitro with exogenous CO treatment and inhibit the activity of heme oxygenase system with zinc protoporphyrin 9. Methods: Oligodendrocyte cultured in vitro were divided into control group, CO group and zinc protoporphyrin group 9. CO group and zinc protoporphyrin group 9 cells were cultured in sealed chamber containing 1% CO. Before CO treatment, zinc protoporphyrin group 9 cells were added to culture medium for 10 microns. Ol/L zinc protoporphyrin 9.Firstly, the expression levels of Nogo-A mR NA and protein in cultured cells at 6, 24 and 48 h were compared, and the differences of expression levels of Nogo-A mR NA and protein between CO and zinc protoporphyrin 9 cells at the highest expression levels were detected. The expression level of Nogo-A m RNA and protein in the nine zinc protoporphyrin groups was higher than that in the CO group at 24 h after culture. It can inhibit the expression level of Nogo-A m RNA and protein.
【作者单位】：大连市第三人民医院神经内科;大连医科大学;大连市第三人民医院综合病房;大连市中心医院神经内科;大连市中心医院康复科;
【基金】：大连市卫生局2008年度科研计划项目~~
【分类号】：R595.1;R747.9

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