大鼠卵巢去势后颌骨和股骨骨髓基质干细胞性能观察
发布时间：2018-08-22 07:20
【摘要】：目的观察去卵巢骨质疏松大鼠颌骨及股骨来源的骨髓间充质干细胞增殖及成骨分化能力的差异。方法雌性SD大鼠双侧卵巢切除建立骨质疏松模型。培养正常和骨质疏松状态下大鼠颌骨及股骨来源的骨髓间充质干细胞。观察细胞形态,进行MTT和CCK-8实验比较四组干细胞的增殖能力。制备四组干细胞膜片与TCP复合进行大网膜移植,术后6w行组织学观察。结果去卵巢组BMSCs在同等时间条件下与未去卵巢组比较,初期增殖速度无显著性差异,3 d后增殖速度则慢于健康组(P0.05)。颌骨和股骨BMSCs在健康骨质环境下,细胞的OD值各时间段均未出现统计学差异;骨质疏松状态下颌骨来源BMSCs后期的增殖能力明显优于股骨来源的BMSCs(P0.05);四种膜片的大网膜移植结果显示体内移植6w后在的TCP诱导下均可形成不均质的矿化物沉积和纤维样组织穿通,似牙周膜/牙骨质复合体样结构;去卵巢组和未去卵巢组颌骨来源BMSCs组较同等条件下股骨来源BMSCs再生矿化物能力强,牙骨质/牙周膜样复合体的形成更满意。结论骨质疏松对BMSCs的增殖和分化可能存在着部位因素影响且具有一定的时间依赖性。
[Abstract]:Objective to observe the difference of proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) derived from mandible and femur of ovariectomized rats. Methods Osteoporosis model was established by bilateral ovariectomy in female SD rats. Bone marrow mesenchymal stem cells derived from rat jaws and femur were cultured under normal and osteoporosis condition. The cell morphology was observed and the proliferative ability of stem cells in four groups was compared by MTT and CCK-8. Four groups of stem cell membranes were prepared and combined with TCP for omentum transplantation. Histological observation was made 6 weeks after operation. Results compared with the unovariectomized group, the initial proliferation rate of BMSCs in ovariectomized group was lower than that in the healthy group after 3 days (P0.05), but there was no significant difference in the initial proliferation rate between the ovariectomized group and the non-ovariectomized group. There was no significant difference in OD value between jaw bone and femur BMSCs in healthy bone environment. The proliferative ability of mandibular BMSCs in the later stage of osteoporosis was significantly better than that of BMSCs derived from femur (P0.05). The results of four kinds of membrane transplantation of greater omentum showed that under the induction of TCP in vivo, uneven deposition of mineralized matter and perforation of fibrous tissue could be formed after 6 weeks of transplantation in vivo. Ovariectomized and non-ovariectomized BMSCs groups had stronger ability to regenerate mineralized matter than femur derived BMSCs, and the formation of cementum / periodontal membrane-like complex was more satisfactory than that of femur origin. Conclusion Osteoporosis may affect the proliferation and differentiation of BMSCs in a time dependent manner.
【作者单位】： 武警工程大学武警医院急诊科;武警工程大学武警医院口腔中心;西安市第九人民医院口腔科;
【基金】：武警后勤科研项目(wjh20110256)
【分类号】：R580
，
本文编号：2196373
[Abstract]:Objective to observe the difference of proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) derived from mandible and femur of ovariectomized rats. Methods Osteoporosis model was established by bilateral ovariectomy in female SD rats. Bone marrow mesenchymal stem cells derived from rat jaws and femur were cultured under normal and osteoporosis condition. The cell morphology was observed and the proliferative ability of stem cells in four groups was compared by MTT and CCK-8. Four groups of stem cell membranes were prepared and combined with TCP for omentum transplantation. Histological observation was made 6 weeks after operation. Results compared with the unovariectomized group, the initial proliferation rate of BMSCs in ovariectomized group was lower than that in the healthy group after 3 days (P0.05), but there was no significant difference in the initial proliferation rate between the ovariectomized group and the non-ovariectomized group. There was no significant difference in OD value between jaw bone and femur BMSCs in healthy bone environment. The proliferative ability of mandibular BMSCs in the later stage of osteoporosis was significantly better than that of BMSCs derived from femur (P0.05). The results of four kinds of membrane transplantation of greater omentum showed that under the induction of TCP in vivo, uneven deposition of mineralized matter and perforation of fibrous tissue could be formed after 6 weeks of transplantation in vivo. Ovariectomized and non-ovariectomized BMSCs groups had stronger ability to regenerate mineralized matter than femur derived BMSCs, and the formation of cementum / periodontal membrane-like complex was more satisfactory than that of femur origin. Conclusion Osteoporosis may affect the proliferation and differentiation of BMSCs in a time dependent manner.
【作者单位】： 武警工程大学武警医院急诊科;武警工程大学武警医院口腔中心;西安市第九人民医院口腔科;
【基金】：武警后勤科研项目(wjh20110256)
【分类号】：R580
，
本文编号：2196373
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