超重人群血清C1q肿瘤坏死因子相关蛋白12水平的研究
发布时间：2018-08-28 10:38
【摘要】：目的:脂肪组织通过表达和分泌脂肪因子调节能量代谢。肥胖状态下,脂肪因子的异常分泌促进代谢性及心血管疾病的发生发展。C1q肿瘤坏死因子相关蛋白12(complement-C1q/TNF-related protein 12,CTRP12)是一种新型脂肪因子,属于补体C1q/肿瘤坏死因子相关蛋白超家族,在调节代谢及心血管功能方面发挥积极作用。研究发现,肥胖患者血清中CTRP12的水平降低。进一步研究其机制发现CTRP12通过抑制脂肪组织炎性反应改善糖代谢。最新数据显示,近40%男性及30%女性属于超重范畴,表明超重在人群中占有很大比例。本研究旨在检测超重和肥胖人群血清CTRP12的水平,并分析CTRP12与生化指标的关系,探讨这一新型脂肪因子在超重人群中的变化及临床意义。方法:选取2016年3月-6月于河北省人民医院体检中心进行体检的人群为受试对象,共282人纳入研究,其中正常组(CON)81例、超重组(OW)93例、肥胖组(OB)108例。应用全自动生化分析仪检测血糖、血脂等生化指标;应用电化学发光法检测血清胰岛素水平;应用高压液相色谱法测定糖化血红蛋白(glycosylated hemoglobin,Hb A1c)水平;应用酶联免疫吸附法(enzyme-linked immunsorbent assay,ELISA)试剂盒检测CTRP12、脂联素(adiponectin,APN)及抵抗素(Resistin)的水平;采用稳态模型胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)评估受试者胰岛素敏感性。采用SPSS21.0软件对上述数据进行统计分析。结果:1三组一般资料与生化指标的比较1.1三组受试者一般资料比较比较三组间性别、年龄、体重指数(BMI)、腰围(WC)、腰臀比(WHR)以及血压的差异。结果提示,与CON组相比,OW组受试者BMI、WC及WHR显著升高(P0.001),OB组SBP、DBP、BMI、WC和WHR显著升高(P0.001)。此外,OW组SBP、DBP、BMI、WC和WHR均低于OB组(P0.05)。1.2三组受试者生化指标比较与CON组比,OW及OB组FBG、2h PBG、FINS、2h INS、Hb A1c、TG、LDL-C显著升高,HDL-C及Apo A1显著降低(P0.05)。OB组TC较CON组显著升高。OW组FBG、2h PBG、FINS、2h INS、Hb A1c、TG较OB组显著降低(P0.05),HDL-C及Apo A1显著升高(P0.05)。TC及LDL-C在OW与OB组间无显著差异。1.3三组胰岛素敏感性指标比较与CON组比,OW和OB组HOMA-IR均显著升高(P0.001)。与OB组相比,OW组HOMA-IR显著降低(P0.001)。2各组受试者血清脂肪因子水平的比较2.1血清脂肪因子水平的比较应用方差分析比较三组人群血清脂肪因子(CTRP12、APN、Resistin)水平的差异。与CON组比较,CTRP12的水平在OW组(P=0.03)及OB组(P=0.001)显著降低;APN水平在OW组(P=0.001)及OB组(P0.001)显著降低,而Resistin水平在OW组(P=0.034)及OB组(P0.001)显著升高。与OB组比较,OW组血清CTRP12和APN水平有升高趋势,血清Resistin水平显著降低(P=0.04)。2.2按性别分层比较血清脂肪因子水平本研究纳入的研究对象中,包含男性133人,女性149人。应用Mann-Whitney U非参数检验对不同性别亚组间血清脂肪因子水平进行比较。结果提示血清CTRP12,APN,Resistin水平无性别差异。3血清CTRP12与各生化指标的关联分析3.1血清CTRP12与一般资料及各生化指标的相关性应用Pearson或Spearman秩相关系数评价CTRP12与年龄、BMI、WC等一般资料以及各生化指标之间的相关性。结果提示血清CTRP12水平与年龄、BMI、WC、WHR、SBP、FBG、2h PBG、Hb A1c、HOMA-IR、TC、TG、LDL-C呈显著的负相关关系。3.2血清CTRP12水平的影响因素分析应用多元线性逐步回归分析,以CTRP12作为因变量,观察BMI、WC、WHR、FBG、Hb A1c、HOMA-IR、TG等对CTRP12的影响,结果提示WC是血清CTRP12的重要影响因素(P=0.001)。结论:1血清CTRP12水平不仅在肥胖患者,而且在超重者已经显著降低,提示其可能为肥胖的早期预测指标。2 CTRP12与年龄、BMI、WC、WHR、HOMA-IR、FBG、2h PBG、TC、TG、LDL-C、Hb A1c和SBP呈负相关,且WC是CTRP12的重要影响因素。
[Abstract]:Objective: Adipose tissue regulates energy metabolism through the expression and secretion of adipocytokines. Abnormal secretion of adipocytokines promotes the development of metabolic and cardiovascular diseases under obesity. Complement-C1q/TNF-related protein 12 (CTRP12) is a novel adipocytokine, belonging to complement C1q/tumor damage. The death factor-related protein superfamily plays an active role in regulating metabolism and cardiovascular function. Studies have found that the level of serum CTRP12 in obese patients decreases. Further studies have found that CTRP12 improves glucose metabolism by inhibiting inflammatory responses in adipose tissue. Recent data show that nearly 40% of men and 30% of women are overweight. The purpose of this study was to detect the levels of serum CTRP12 in overweight and obese people, and to analyze the relationship between CTRP12 and biochemical indicators, and to explore the changes and clinical significance of this new fat factor in overweight people. A total of 282 subjects were included in the study, including 81 normal subjects (CON), 93 overweight subjects (OW) and 108 obese subjects (OB). The levels of CTRP12, adiponectin (APN) and resistin (Resistin) were detected by enzyme-linked immunosorbent assay (ELISA), and insulin sensitivity was assessed by homeostasis model of insulin resistance (HOMA-IR). SPSS21.0 software was used to analyze the above data. Results: 1 Comparison of general data and biochemical indicators of three groups 1.1 Comparison of general data of three groups of subjects sex, age, body mass index (BMI), waist circumference (WC), waist-hip ratio (WHR) and blood pressure differences between the three groups. In addition, SBP, DBP, BMI, WC and WHR in OW group were lower than those in OB group (P 0.05). 1.2 Compared with CON group, FBG, 2H PBG, FINS, 2H INS, Hb A1c, TG, LDL-C, HDL-C and Apo A1 in OW group were significantly higher than those in CON group (P 0.05). There was no significant difference between OW and OB groups in TC and LDL-C. Compared with C ON group, HOMA-IR in OW and OB groups were significantly increased (P 0.001). Compared with OB group, HOMA-IR in OW and OB group was significantly decreased (P 0.05). Comparison of serum adipokine levels 2.1 Comparison of serum adipokine levels using variance analysis of three groups of people with serum adipokine (CTRP12, APN, Resistin) levels. Compared with the CON group, CTRP12 levels in OW group (P = 0.03) and OB group (P = 0.001) were significantly lower; APN levels in OW group (P = 0.001) and OB group (P = 0.001) were significantly lower, while Resistin levels in OW group (P = 0.001) were significantly lower. Compared with OB group, serum CTRP12 and APN levels in OW group increased, serum Resistin levels decreased significantly (P = 0.04). 2. The serum levels of CTRP12, APN and Resistin were not significantly different between the sexes. 3 Correlation analysis of serum CTRP12 with biochemical indexes 3.1 Correlation analysis of serum CTRP12 with general data and biochemical indexes Pearson or Spearman rank correlation coefficient was used to evaluate CTRP12 with age, BMI and WC. The results showed that serum CTRP12 levels were negatively correlated with age, BMI, WC, WHR, SBP, FBG, 2H PBG, Hb A1c, HOMA-IR, TC, TG, LDL-C. 3.2 The influencing factors of serum CTRP12 levels were analyzed by multivariate linear stepwise regression analysis. BMI, WC, WHR, FBG, Hb A1c, HOMA-IR, TC, TG and LDL-C were observed as dependent variables. Conclusion: 1. Serum CTRP12 levels have been significantly decreased not only in obese patients, but also in overweight patients, suggesting that it may be an early predictor of obesity. 2. CTRP12 is negatively correlated with age, BMI, WC, WHR, HOMA-IR, FBG, 2H PBG, TC, TG, LDL-C, Hb A1c and SBP. WC is an important influence factor of CTRP12.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R589.2
[Abstract]:Objective: Adipose tissue regulates energy metabolism through the expression and secretion of adipocytokines. Abnormal secretion of adipocytokines promotes the development of metabolic and cardiovascular diseases under obesity. Complement-C1q/TNF-related protein 12 (CTRP12) is a novel adipocytokine, belonging to complement C1q/tumor damage. The death factor-related protein superfamily plays an active role in regulating metabolism and cardiovascular function. Studies have found that the level of serum CTRP12 in obese patients decreases. Further studies have found that CTRP12 improves glucose metabolism by inhibiting inflammatory responses in adipose tissue. Recent data show that nearly 40% of men and 30% of women are overweight. The purpose of this study was to detect the levels of serum CTRP12 in overweight and obese people, and to analyze the relationship between CTRP12 and biochemical indicators, and to explore the changes and clinical significance of this new fat factor in overweight people. A total of 282 subjects were included in the study, including 81 normal subjects (CON), 93 overweight subjects (OW) and 108 obese subjects (OB). The levels of CTRP12, adiponectin (APN) and resistin (Resistin) were detected by enzyme-linked immunosorbent assay (ELISA), and insulin sensitivity was assessed by homeostasis model of insulin resistance (HOMA-IR). SPSS21.0 software was used to analyze the above data. Results: 1 Comparison of general data and biochemical indicators of three groups 1.1 Comparison of general data of three groups of subjects sex, age, body mass index (BMI), waist circumference (WC), waist-hip ratio (WHR) and blood pressure differences between the three groups. In addition, SBP, DBP, BMI, WC and WHR in OW group were lower than those in OB group (P 0.05). 1.2 Compared with CON group, FBG, 2H PBG, FINS, 2H INS, Hb A1c, TG, LDL-C, HDL-C and Apo A1 in OW group were significantly higher than those in CON group (P 0.05). There was no significant difference between OW and OB groups in TC and LDL-C. Compared with C ON group, HOMA-IR in OW and OB groups were significantly increased (P 0.001). Compared with OB group, HOMA-IR in OW and OB group was significantly decreased (P 0.05). Comparison of serum adipokine levels 2.1 Comparison of serum adipokine levels using variance analysis of three groups of people with serum adipokine (CTRP12, APN, Resistin) levels. Compared with the CON group, CTRP12 levels in OW group (P = 0.03) and OB group (P = 0.001) were significantly lower; APN levels in OW group (P = 0.001) and OB group (P = 0.001) were significantly lower, while Resistin levels in OW group (P = 0.001) were significantly lower. Compared with OB group, serum CTRP12 and APN levels in OW group increased, serum Resistin levels decreased significantly (P = 0.04). 2. The serum levels of CTRP12, APN and Resistin were not significantly different between the sexes. 3 Correlation analysis of serum CTRP12 with biochemical indexes 3.1 Correlation analysis of serum CTRP12 with general data and biochemical indexes Pearson or Spearman rank correlation coefficient was used to evaluate CTRP12 with age, BMI and WC. The results showed that serum CTRP12 levels were negatively correlated with age, BMI, WC, WHR, SBP, FBG, 2H PBG, Hb A1c, HOMA-IR, TC, TG, LDL-C. 3.2 The influencing factors of serum CTRP12 levels were analyzed by multivariate linear stepwise regression analysis. BMI, WC, WHR, FBG, Hb A1c, HOMA-IR, TC, TG and LDL-C were observed as dependent variables. Conclusion: 1. Serum CTRP12 levels have been significantly decreased not only in obese patients, but also in overweight patients, suggesting that it may be an early predictor of obesity. 2. CTRP12 is negatively correlated with age, BMI, WC, WHR, HOMA-IR, FBG, 2H PBG, TC, TG, LDL-C, Hb A1c and SBP. WC is an important influence factor of CTRP12.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R589.2
【相似文献】
相关期刊论文 前10条
1 潘华;余叶蓉;;脂肪因子对血管内皮细胞功能的影响[J];现代临床医学;2007年01期
2 谢谦;张帆;胡小平;;脂联素-一种负性脂肪因子[J];临床和实验医学杂志;2008年08期
3 殷忠;杨晔;;阻塞性睡眠呼吸暂停与脂肪因子[J];医学综述;2010年18期
4 竹井，
本文编号：2209143
本文链接：https://www.wllwen.com/yixuelunwen/nfm/2209143.html
最近更新
教材专著
