脊髓小胶质细胞酪氨酸家族激酶在糖尿病神经痛中的作用
发布时间：2018-08-31 11:49
【摘要】：目的:探讨脊髓背角小胶质细胞上酪氨酸家族激酶(SFKs)在糖尿病神经痛中的作用。方法:采用雄性SD大鼠,体重200~220 g,免疫荧光单染检测腹腔注射链脲佐菌素(STZ)后7、14、28 d脊髓背角p-SFKs的表达水平,双染观察其表达部位;同时在给予STZ前鞘内注射SFKs抑制剂PP2,行为学检测大鼠50%机械撤足阈值的变化。结果 :与对照组比较,腹腔注射STZ后第1天,大鼠血糖水平开始升高(P0.001),持续至少28 d(P0.001);机械痛撤足逐渐下降(P0.05),28 d达到最低(P0.001);同时腹腔注射STZ后7、14、28 d,脊髓背角p-SFKs的表达水平明显升高(均P0.01);只表达于小胶质细胞中,神经元和星型胶质细胞中无表达。腹腔给予STZ前鞘内注射PP2可防止STZ诱导的机械痛敏。结论:脊髓背角小胶质细胞SFKs可能在糖尿病神经痛中发挥重要作用。
[Abstract]:Aim: to investigate the role of tyrosine family kinase (SFKs) on spinal dorsal horn microglia in diabetic neuralgia. Methods: the expression of p-SFKs in spinal dorsal horn of male SD rats, weighing 200 ~ 220g, was detected by immunofluorescence single staining on the 28th day after intraperitoneal injection of streptozotocin (STZ). The expression sites of p-SFKs in spinal dorsal horn were observed by double staining. At the same time, the behavioral changes of 50% mechanical withdrawal threshold of rats were detected by intrathecal injection of SFKs inhibitor PP2, before STZ. Results: compared with the control group, the first day after intraperitoneal injection of STZ, The blood glucose level of rats began to rise (P0.001) and lasted for at least 28 days (P0.001); the mechanical pain gradually decreased (P0.05) and reached the lowest level at 28 days (P0.001); at the same time, the expression of p-SFKs in the dorsal horn of spinal cord increased significantly (all P0.01) at 714 days after intraperitoneal injection of STZ, and was only expressed in microglia cells. No expression was found in neurons and astrocytes. Intrathecal injection of PP2 before STZ could prevent mechanical pain induced by STZ. Conclusion: SFKs of spinal dorsal horn microglia may play an important role in diabetic neuralgia.
【作者单位】： 广州医科大学附属第二医院疼痛科中华疼痛学会第一临床中心;
【基金】：国家自然科学基金资助项目(编号:31100805) 广州市珠江科技新星项目(编号:2012J2200036) 广州市西医一般引导项目(编号:20141A011081)
【分类号】：R587.2
，
本文编号：2214895
[Abstract]:Aim: to investigate the role of tyrosine family kinase (SFKs) on spinal dorsal horn microglia in diabetic neuralgia. Methods: the expression of p-SFKs in spinal dorsal horn of male SD rats, weighing 200 ~ 220g, was detected by immunofluorescence single staining on the 28th day after intraperitoneal injection of streptozotocin (STZ). The expression sites of p-SFKs in spinal dorsal horn were observed by double staining. At the same time, the behavioral changes of 50% mechanical withdrawal threshold of rats were detected by intrathecal injection of SFKs inhibitor PP2, before STZ. Results: compared with the control group, the first day after intraperitoneal injection of STZ, The blood glucose level of rats began to rise (P0.001) and lasted for at least 28 days (P0.001); the mechanical pain gradually decreased (P0.05) and reached the lowest level at 28 days (P0.001); at the same time, the expression of p-SFKs in the dorsal horn of spinal cord increased significantly (all P0.01) at 714 days after intraperitoneal injection of STZ, and was only expressed in microglia cells. No expression was found in neurons and astrocytes. Intrathecal injection of PP2 before STZ could prevent mechanical pain induced by STZ. Conclusion: SFKs of spinal dorsal horn microglia may play an important role in diabetic neuralgia.
【作者单位】： 广州医科大学附属第二医院疼痛科中华疼痛学会第一临床中心;
【基金】：国家自然科学基金资助项目(编号:31100805) 广州市珠江科技新星项目(编号:2012J2200036) 广州市西医一般引导项目(编号:20141A011081)
【分类号】：R587.2
，
本文编号：2214895
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