间歇性断食对老年前期肥胖大鼠肠道菌群及代谢的影响

发布时间：2018-09-04 17:53

【摘要】：目的研究间歇性断食对老年前期肥胖大鼠代谢和肠道菌群的影响。方法雌性Wistar大鼠经42周高脂高糖饲料饲养造模,根据体质量选取模型鼠进行间歇性断食干预。干预方法为每2周断食72 h,总干预时间18周。干预后进行口服葡萄糖耐量试验、血脂4项检测。收集粪便,通过Illumina高通量测序检测16S r RNA基因V4可变区,运用QIIME及LEf Se分析肠道菌群。结果间歇性断食组体质量相对于模型对照组显著下降(P0.01);高密度脂蛋白胆固醇和低密度脂蛋白胆固醇均显著下降(P0.05);空腹血糖显著上升(P0.01);葡萄糖耐量测试曲线下面积显著高于模型对照组,糖耐量减退(P0.05);HE染色显示间歇性断食轻度减少肝脏脂肪变性。肠道菌群结果显示,断食组肠道菌群得到显著改善,具体表现为YS2、RF32、Helicobacteraceae(螺杆菌科)增加,Lactobacillus(乳杆菌属)、Roseburia(罗氏菌属)、Erysipelotrichaceae(韦荣球菌科)、Ralstonia(青枯菌属)、Bradyrhizobiaceae(慢生根瘤菌科)和RF39减少。Spearman相关性分析发现Bradyrhizobiaceae与总胆固醇、高密度脂蛋白胆固醇呈正相关;大鼠体质量与RF39呈负相关。结论间歇性断食能改善肠道菌群,降低老年前期肥胖大鼠体质量和血脂水平,但对糖代谢有不良影响。
[Abstract]:Objective to study the effects of intermittent food deprivation on metabolism and intestinal flora of obese rats. Methods female Wistar rats were fed with high fat and high sugar forage for 42 weeks. The intervention method was 72 hours every 2 weeks, and the total intervention time was 18 weeks. After intervention, oral glucose tolerance test and 4 items of blood lipids were tested. Feces were collected and the V4 variable region of 16s r RNA gene was detected by Illumina high-throughput sequencing. The intestinal flora was analyzed by QIIME and LEf Se. Results compared with the model control group, the body mass of intermittent diet group decreased significantly (P0.01), the high density lipoprotein cholesterol and low density lipoprotein cholesterol decreased significantly (P0.05), the fasting blood glucose increased significantly (P0.01), and the area under the curve of glucose tolerance test was significantly increased. Significantly higher than the model control group, Impaired glucose tolerance (P0.05) and HE staining showed that intermittent food deprivation slightly reduced hepatic steatosis. The results of intestinal microflora showed that the intestinal flora of the diet group was significantly improved. YS2,RF32,Helicobacteraceae (Helicobacteriaceae) increased Lactobacillus (Lactobacillus) Roseburia (Roxburia) Erysipelotrichaceae (Veronella) and Bradyrhizobiaceae (Bradyrhizobiaceae) and RF39 decreased. Spearman correlation analysis showed that Bradyrhizobiaceae was positively correlated with total cholesterol and high density lipoprotein cholesterol. There was a negative correlation between body mass and RF39 in rats. Conclusion intermittent feeding can improve intestinal flora, decrease body mass and blood lipid level of obese rats, but have a negative effect on glucose metabolism.
【作者单位】：南方医科大学公共卫生学院环境卫生学系;南方医科大学珠江医院检验医学部;南方医科大学南方医院神经内科;
【基金】：国家自然科学基金优秀青年基金(31322003)~~
【分类号】：R589.2

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