骨形态发生蛋白-7基因变异与太原地区汉族人群糖尿病及胰岛素抵抗关联研究
发布时间：2018-09-07 20:15
【摘要】：目的:探讨骨形态发生蛋白-7(bone morphogenetic protein 7,BMP7)基因变异与太原地区汉族人群2型糖尿病、胰岛素抵抗的关系,从分子遗传学的角度和水平阐明太原地区汉族人群易患糖尿病的遗传机制,为太原地区汉族人群2型糖尿病的防治提供一定的遗传学证据。方法:选用以流行病学调查为基础的病例-对照研究,选取173名(男性104人、女性69人)太原地区汉族人作为研究对象,按照1999年WHO制订的2型糖尿病诊断标准,将研究对象分成糖尿病组(90例,男性56例、女性34例)和正常对照组(83人,男性48人、女性35人)。选取2个具有代表性的BMP7基因位点在研究对象中进行SNa Pshot-PCR基因型鉴定,通过己糖激酶法测定空腹血糖(FBG,单位mmol/L),化学发光法测定空腹血胰岛素(Fl NS,单位m IU/L),稳态模型评估法(HOMA)计算胰岛素抵抗指数(HOMA-IR=FBG×FINS/22.5),β细胞功能指数(HOMA-β)通过HOMA-β=20×FINS/(FBG-3.5)评估,并分析BMP7基因变异与2型糖尿病及胰岛素抵抗的关联性。结果:1.经过Hardy-Weinberg检验可知:BMP7基因的rs6025422位点和rs17480735位点符合平衡定律(P0.05)。经过卡方检验可知rs6025422基因位点分布在正常对照组和糖尿病组有统计学差异(P0.05),rs17480735基因位点无统计学差异(P0.05)。2.Logistic回归分析结果显示:rs6025422基因位点中GG基因型为糖尿病的保护因素,年龄、BMI为糖尿病的危险因素;3.糖尿病表型资料在基因rs6025422不同基因型组间的比较显示:男性人群中不同基因型组间FBG、FINS、HOMA-IR有统计学差异(P0.05=,HOMA-β无统计学差异(P0.05)。FBG和HOMA-IR在校正P值后差异仍有统计学意义(Pα0.05),经比较可知FBG、FINS、HOMA-IR在基因AA、AG、GG间呈趋势性降低;总人群中FINS和HOMA-IR有统计学差异,HOMA-β无统计学差异(P0.05);女性人群中各指标在基因型之间无统计学差异(P0.05)。结论:1.BMP7基因的rs6025422基因位点中GG基因型为糖尿病的保护因素,年龄、BMI为糖尿病的危险因素;2.糖尿病表型资料在基因rs6025422不同基因型组间的比较显示男性人群中不同基因型组间FBG、FINS、HOMA-IR有统计学差异(P0.05),HOMA-β无统计学差异(P0.05)。FBG和HOMA-IR在校正P值后差异仍有统计学意义(Pα0.05),经比较可知FBG、FINS、HOMA-IR在基因AA、AG、GG间呈趋势性降低;总人群中FINS和HOMA-IR有统计学差异,HOMA-β无统计学差异(P0.05);女性人群中各指标在基因型之间无统计学差异(P0.05)。3.BMP7基因变异可能与太原地区汉族人群2型糖尿病与胰岛素抵抗相关。
[Abstract]:Objective: to investigate the relationship between the variation of bone morphogenetic protein-7 (bone morphogenetic protein 7 (BMP7) gene and type 2 diabetes mellitus and insulin resistance in Taiyuan Han population, and to elucidate the genetic mechanism of the susceptibility to diabetes in Taiyuan Han population from the perspective of molecular genetics. To provide some genetic evidence for the prevention and treatment of type 2 diabetes in Taiyuan Han population. Methods: a case-control study based on epidemiological investigation was conducted. 173 Han Chinese (104 males and 69 females) in Taiyuan were selected as the subjects. The diagnostic criteria for type 2 diabetes were established by WHO in 1999. The subjects were divided into diabetic group (90 cases, male 56 cases, female 34 cases) and normal control group (83 cases, male 48, female 35). Two representative BMP7 loci were selected to identify SNa Pshot-PCR genotypes. Fasting blood glucose (FBG, unit mmol/L) was measured by hexokinase assay, fasting insulin (Fl NS, unit m IU/L was measured by chemiluminescence assay, insulin resistance index (HOMA-IR=FBG 脳 FINS/22.5) was calculated by steady-state model evaluation method (HOMA), and 尾 cell function index (HOMA- 尾) was evaluated by HOMA- 尾 -20 脳 FINS/ (FBG-3.5). The association of BMP7 gene mutation with type 2 diabetes mellitus and insulin resistance was analyzed. The result is 1: 1. Hardy-Weinberg test showed that the rs6025422 and rs17480735 loci of the BMP7 gene obeyed the equilibrium law (P0.05). After chi-square test, the distribution of rs6025422 gene loci in normal control group and diabetes group was statistically different (P0.05). There was no significant difference in rs17480735 gene locus (P0.05). 2. Logistic regression analysis showed that GG genotype in the GG gene locus was the protective factor of diabetes mellitus. Age BMI is a risk factor for diabetes. The comparison of phenotypic data of diabetes mellitus among different genotypes of gene rs6025422 showed that there were significant differences in FBG,FINS,HOMA-IR between different genotypes of male population (P0.05Homa 尾) (P0.05) .FBGs and HOMA-IR were still significantly different after correction of P value (P 伪 0.05). The results showed that FBG,FINS,HOMA-IR decreased gradually between gene AA,AG,GG. There was no statistical difference between FINS and HOMA-IR in the total population (P0.05), and there was no statistical difference among the genotypes in the female population (P0.05). Conclusion the rs6025422 genotype of BMP7 gene is the protective factor of diabetes mellitus and age is the risk factor of diabetes mellitus. The comparison of phenotypic data of diabetes mellitus among different genotypes of gene rs6025422 showed that there was significant difference in FBG,FINS,HOMA-IR between different genotypes of male population (P0.05). There was no significant difference in HOMA- 尾 (P0.05) .FBGs and HOMA-IR remained statistically significant after correcting P value (P 伪 0. 05). The results showed that FBG,FINS,HOMA-IR decreased gradually between gene AA,AG,GG. There was no statistical difference between FINS and HOMA-IR in total population (P0.05), but there was no significant difference between genotype (P0.05). 3. BMP7 gene mutation may be related to type 2 diabetes mellitus and insulin resistance in Taiyuan Han population.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R587.1
本文编号：2229304
[Abstract]:Objective: to investigate the relationship between the variation of bone morphogenetic protein-7 (bone morphogenetic protein 7 (BMP7) gene and type 2 diabetes mellitus and insulin resistance in Taiyuan Han population, and to elucidate the genetic mechanism of the susceptibility to diabetes in Taiyuan Han population from the perspective of molecular genetics. To provide some genetic evidence for the prevention and treatment of type 2 diabetes in Taiyuan Han population. Methods: a case-control study based on epidemiological investigation was conducted. 173 Han Chinese (104 males and 69 females) in Taiyuan were selected as the subjects. The diagnostic criteria for type 2 diabetes were established by WHO in 1999. The subjects were divided into diabetic group (90 cases, male 56 cases, female 34 cases) and normal control group (83 cases, male 48, female 35). Two representative BMP7 loci were selected to identify SNa Pshot-PCR genotypes. Fasting blood glucose (FBG, unit mmol/L) was measured by hexokinase assay, fasting insulin (Fl NS, unit m IU/L was measured by chemiluminescence assay, insulin resistance index (HOMA-IR=FBG 脳 FINS/22.5) was calculated by steady-state model evaluation method (HOMA), and 尾 cell function index (HOMA- 尾) was evaluated by HOMA- 尾 -20 脳 FINS/ (FBG-3.5). The association of BMP7 gene mutation with type 2 diabetes mellitus and insulin resistance was analyzed. The result is 1: 1. Hardy-Weinberg test showed that the rs6025422 and rs17480735 loci of the BMP7 gene obeyed the equilibrium law (P0.05). After chi-square test, the distribution of rs6025422 gene loci in normal control group and diabetes group was statistically different (P0.05). There was no significant difference in rs17480735 gene locus (P0.05). 2. Logistic regression analysis showed that GG genotype in the GG gene locus was the protective factor of diabetes mellitus. Age BMI is a risk factor for diabetes. The comparison of phenotypic data of diabetes mellitus among different genotypes of gene rs6025422 showed that there were significant differences in FBG,FINS,HOMA-IR between different genotypes of male population (P0.05Homa 尾) (P0.05) .FBGs and HOMA-IR were still significantly different after correction of P value (P 伪 0.05). The results showed that FBG,FINS,HOMA-IR decreased gradually between gene AA,AG,GG. There was no statistical difference between FINS and HOMA-IR in the total population (P0.05), and there was no statistical difference among the genotypes in the female population (P0.05). Conclusion the rs6025422 genotype of BMP7 gene is the protective factor of diabetes mellitus and age is the risk factor of diabetes mellitus. The comparison of phenotypic data of diabetes mellitus among different genotypes of gene rs6025422 showed that there was significant difference in FBG,FINS,HOMA-IR between different genotypes of male population (P0.05). There was no significant difference in HOMA- 尾 (P0.05) .FBGs and HOMA-IR remained statistically significant after correcting P value (P 伪 0. 05). The results showed that FBG,FINS,HOMA-IR decreased gradually between gene AA,AG,GG. There was no statistical difference between FINS and HOMA-IR in total population (P0.05), but there was no significant difference between genotype (P0.05). 3. BMP7 gene mutation may be related to type 2 diabetes mellitus and insulin resistance in Taiyuan Han population.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R587.1
【参考文献】
相关期刊论文 前2条
1 金军华;老年糖尿病与衰老和肥胖[J];中国老年学杂志;2003年10期
2 陈蕾,贾伟平,项坤三,陆俊茜,包玉倩,薛凤仙,陆蔚;肥胖者胰岛素抵抗与总体脂、局部体脂关系的研究[J];中华内分泌代谢杂志;2001年05期
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