糖尿病合并去势大鼠骨微结构退化的研究

发布时间：2018-09-12 14:29

【摘要】：目的探讨糖尿病合并去势大鼠骨微结构的改变。方法 6月龄雌性大鼠40只,随机分为对照组、假手术组、去势组、糖尿病组和糖尿病合并去势组。6 w和15 w后应用Micro-CT测定股骨检测骨密度和骨微结构;利用Van Gieson胶原纤维染色法观察胶原纤维沉积变化;HE染色分析大鼠股骨头骨小梁厚度(Tb.Th)和骨小梁间距(Tb.Sp)变化。结果与对照组相比,去势6 w和15 w组骨β-胶原降解产物水平和Tb.Sp明显升高,骨密度值和Tb.Th明显下降。去势15 w组骨胶原纤维明显下降,差异有统计学意义(P0.05)。糖尿病组15 w松质骨β-胶原降解产物水平、胶原纤维和Tb.SP明显升高,骨密度值、胶原纤维和Tb.Th明显下降,差异有统计学意义(P0.05)。糖尿病合并去势组6 w和15 w组松质骨β-胶原降解产物水平、胶原纤维和Tb.Sp明显升高,骨密度值和Tb.Th明显下降,差异有统计学意义(P0.05)。结论糖尿病合并去势早期是以去势为主导致的骨质疏松,后期则由糖尿病缓慢的骨分解加持,骨质疏松继续加重。
[Abstract]:Objective to investigate the changes of bone microstructure in diabetic castrated rats. Methods Forty 6-month-old female rats were randomly divided into control group, sham operation group, castration group, diabetes mellitus group and diabetic combined castration group. Bone mineral density (BMD) and bone microstructure were measured by Micro-CT after 6 weeks and 15 weeks. The changes of collagen deposition were observed by Van Gieson staining. The changes of trabecular thickness (Tb.Th) and trabecular spacing (Tb.Sp) of femoral head in rats were analyzed by HE staining. Results compared with the control group, bone 尾 -collagen degradation products and Tb.Sp were significantly increased, bone mineral density (BMD) and Tb.Th were significantly decreased in ovariectomized groups at 6 and 15 weeks. In castration group, collagen fibers decreased significantly at 15 weeks (P0.05). In the diabetic group, 尾 -collagen degradation product level, collagen fiber and Tb.SP increased significantly, bone mineral density, collagen fiber and Tb.Th decreased significantly in the diabetic group at 15 weeks, the difference was statistically significant (P0.05). The levels of 尾 -collagen degradation products, collagen fibers and Tb.Sp, bone mineral density (BMD) and Tb.Th decreased significantly in castrated diabetic group at 6 and 15 weeks (P0.05). Conclusion Osteoporosis is the main cause of Ovariectomization in the early stage of diabetes mellitus, and osteoporosis is aggravated in the later stage by the slow osteolysis of diabetes mellitus.
【作者单位】：福建莆田学院临床医学院;
【分类号】：R580;R587.2

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