燃煤污染型氟中毒大鼠脑组织NOS活性及NO含量改变

发布时间：2018-09-19 07:05

【摘要】：目的探讨燃煤型氟中毒对大鼠脑组织一氧化氮合酶(NOS)活性及一氧化氮(NO)含量影响。方法24只SD大鼠随机分为3组,对照组、低、高氟组。染氟组以地氟病区燃煤烘烤的玉米为主要饲料,复制氟中毒动物模型,6个月后用氟离子选择电极法检测动物尿及脑氟含量,比色法测定脑组织NOS活性,硝酸还原酶法测定脑组织NO含量。结果染氟6个月时,低、高氟组大鼠尿氟为(2.87±0.12)、(5.27±0.15)mg/L,脑氟为(1.14±0.04)、(1.79±0.04)mg/kg,明显高于对照组[尿氟(1.68±0.02)mg/L,脑氟(0.52±0.05)mg/kg],差异有统计学意义(P0.05);低氟组大鼠脑组织白细胞/巨噬细胞性NOS(iNOS)活性[(8.04±3.90)k U/g]与低、高氟组大鼠脑组织中NO含量[分别为(3.13±0.63)、(4.25±0.66)μmol/L]明显高于对照组[iNOS活性(4.02±0.20)k U/g、NO含量(2.29±0.46)μmol/L],差异有统计学意义(P0.05)。结论饲喂燃煤型氟中毒病区燃煤烘烤的饲料可致大鼠氟中毒,导致大鼠脑组织中iNOS活性及NO含量升高。
[Abstract]:Objective to investigate the effect of coal-burning fluorosis on the activity of nitric oxide synthase (NOS) and the content of nitric oxide (NO) in rat brain. Methods 24 SD rats were randomly divided into 3 groups: control group, low fluoride group and high fluoride group. In fluorine-exposed group, the animal model of fluorosis was made from corn roasted by coal burning in endemic fluorosis area. After 6 months, the contents of fluoride in urine and brain were detected by fluorine ion selective electrode method, and the activity of NOS in brain tissue was determined by colorimetric method. The content of NO in brain tissue was determined by nitrate reductase method. Results after 6 months of fluoride exposure, the urinary fluorine of rats in the high fluoride group was (2.87 卤0.12), (5.27 卤0.15) mg/L, and (1.14 卤0.04), (, 1.79 卤0.04) mg/kg, which was significantly higher than that in the control group [(1.68 卤0.02) mg/L, (0.52 卤0.05) mg/kg], the difference was statistically significant (P0.05), and the activity of leukocyte / macrophage NOS (iNOS) in the low fluorine group [(8.04 卤3.90) k U / g] was significantly higher than that in the control group (P 0.05). The content of NO [(3.13 卤0.63), (4.25 卤0.66) 渭 mol/L] in high fluoride group was significantly higher than that in control group [iNOS activity (4.02 卤0.20) k U / g no (2.29 卤0.46) 渭 mol/L] (P0.05). Conclusion the feed fed with coal burning in the endemic areas of coal-burning fluorosis can lead to fluorosis in rats and increase the activity of iNOS and the content of NO in brain tissue of rats.
【作者单位】：贵州医科大学病理教研室;贵州医科大学附属肿瘤医院病理科;贵州省电力职工医院病理科;贵州医科大学分子生物学重点实验室;
【基金】：高等学校博士学科点专项科研基金-新教师类联合资助课题(教育部20115215120003) 国家自然科学基金(81460482)
【分类号】：R599

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