模拟高海拔不同时间大鼠肺组织的病理变化研究

发布时间：2018-09-19 17:10

【摘要】：目的:观察高海拔(5000m)不同时间大鼠的肺泡壁厚度、肺内细小动脉壁厚度、细小血管周围有无淋巴细胞浸润、肺气肿改变等病理变化,研究高海拔低压、低氧环境对大鼠肺组织结构的影响。方法:将48只雄性Wistar大鼠随机分成低海拔对照组(海拔1500m)、高海拔1天组、高海拔3天组、高海拔7天组、高海拔15天组、高海拔30天组,每组样本量均为8只,取肺组织制作成病理切片,经HE染色后,观察大鼠的肺泡壁厚度、肺内细小动脉壁厚度、细小血管周围淋巴细胞浸润、肺气肿改变等病理变化。结果:(1)高海拔组与低海拔对照组肺组织病理变化比较提示低氧会导致肺组织结构的破坏;(2)随着大鼠在低压氧舱停留时间的延长,出现肺泡壁增宽、肺内细小动脉壁增厚、细小血管周围出现淋巴细胞浸润、肺气肿改变的大鼠数目比例会逐渐升高;(3)当比例升高到一定程度,大鼠会发生低氧适应,肺泡壁增宽、肺内细小血管周围淋巴细胞浸润、肺气肿的发生率又会逐渐下降。结论:高海拔低氧环境下,大鼠肺组织结构会随着生活时间的延长出现不同程度的破坏,当大鼠低氧适应后,其肺组织会获得对低氧的耐受性,在一定程度上减轻了对肺组织的破坏。
[Abstract]:Objective: to observe the thickness of alveolar wall, the thickness of pulmonary arterioles, the infiltration of lymphocytes and the changes of emphysema in rats at high altitude (5000m). Effects of hypoxia on lung tissue structure in rats. Methods: 48 male Wistar rats were randomly divided into low altitude control group (1 500 m), high altitude 1 day group, high altitude 3 day group, high altitude 7 day group, high altitude 15 day group, high altitude 30 days group. After HE staining, the thickness of alveolar wall, the thickness of pulmonary arterioles, the infiltration of lymphocytes around small blood vessels and the changes of emphysema were observed. Results: (1) the pathological changes of lung tissue in the high altitude group and the low altitude control group showed that hypoxia would lead to the destruction of the lung tissue structure, (2) with the prolongation of the staying time of the rats in the hypobaric oxygen chamber, the alveolar wall widened and the pulmonary arterioles thickened. Lymphocytic infiltration appeared around the small blood vessels, and the proportion of rats with emphysema would gradually increase. (3) when the proportion increased to a certain extent, the rats would develop hypoxic adaptation, the alveolar wall widened, and the lymphocytes infiltrated around the small blood vessels in the lung. The incidence of emphysema will gradually decrease again. Conclusion: at high altitude and hypoxic environment, the lung tissue structure of rats will be damaged in varying degrees with the prolongation of life time, and the lung tissue will gain tolerance to hypoxia after hypoxic adaptation. The damage to lung tissue was alleviated to some extent.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R594.3

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