MicroRNA-3146在急性痛风性关节炎的表达研究

发布时间：2018-10-05 15:30

【摘要】：目的本课题主要研究micro RNA-3146(miR-3146)在急性痛风性关节炎患者的表达水平,分析其对痛风的诊断价值,并研究其与关键炎症因子的相关关系。方法入选2015年1月至12月在青大附院就诊的急性痛风性关节炎、单纯高尿酸血症(HUA)患者各31例,另随机选取健康对照32例。收集患者临床信息,采集外周静脉血测空腹血糖、尿酸、肝功、肾功等指标;采用逆转录PCR方法检测血浆mi R-3146水平,并采用ELISA法检测血浆TNF-α和IL-1β水平。利用统计软件分析mi R-3146在急性痛风性关节炎组、HUA组和正常对照组之间的表达差异,采用ROC曲线评估其对急性痛风性关节炎的诊断意义,分析mi R-3146与TNF-α、IL-1β的相关关系,并分析秋水仙碱治疗是否可改变患者血浆mi R-3146水平。结果急性痛风性关节炎患者血浆miR-3146水平明显高于单纯HUA患者(P=0.002)和健康对照者(P=0.0002)。急性痛风性关节炎组和HUA组的血浆mi R-3146水平与年龄、血压、血脂、肝功和肾功等指标均无相关性(P0.05)。急性痛风性关节炎患者血浆中mi R-3146水平与血浆TNF-α水平呈正相关(Spearman r=0.55,P=0.014),血浆mi R-3146水平与血浆IL-1β水平也呈正相关(Spearman r=0.53,P=0.002)。ROC曲线显示血浆mi R-3146水平对急性痛风性关节炎有一定的诊断价值,曲线下面积为0.725(95%CI:0.596-0.854)。急性痛风性关节炎患者经秋水仙碱治疗后血浆中mi R-3146水平较治疗前明显降低(P=0.026)。生物信息学预测的mi R-3146可能的靶基因包括ADAMTS4、ADAMTS19、SLC16A1、CXCR6等基因。结论mi R-3146在急性痛风性关节炎患者血浆中呈特异性高表达,其表达水平与TNF-α、IL-1β水平存在正相关关系,说明mi R-3146可能参与了痛风的发病过程。血浆mi R-3146对急性痛风性关节炎的有一定的诊断价值。秋水仙碱可降低急性痛风性关节炎患者血浆中mi R-3146的表达水平。
[Abstract]:Objective to study the expression level of micro RNA-3146 (miR-3146) in patients with acute gouty arthritis, analyze its diagnostic value and study the relationship between miR-3146 and key inflammatory factors. Methods from January to December, 2015, 31 patients with acute gouty arthritis, 31 patients with hyperuricemia (HUA) and 32 healthy controls were selected. Clinical information was collected, fasting blood glucose, uric acid, liver function and renal function were measured by peripheral venous blood samples, plasma mi R-3146 levels were detected by reverse transcription PCR method, plasma TNF- 伪 and IL-1 尾 levels were detected by ELISA method. The expression of mi R-3146 in patients with acute gouty arthritis was analyzed by statistical software. The significance of mi R-3146 in diagnosis of acute gouty arthritis was evaluated by ROC curve, and the correlation between mi R-3146 and TNF- 伪 IL-1 尾 was analyzed. Whether colchicine treatment can change plasma mi R-3146 level was analyzed. Results Plasma miR-3146 levels in patients with acute gouty arthritis were significantly higher than those in patients with HUA alone (P0. 002) and healthy controls (P0. 0002). There was no correlation between plasma mi R-3146 level and age, blood pressure, blood lipid, liver function and renal function in acute gouty arthritis group and HUA group (P0.05). There was a positive correlation between plasma mi R-3146 level and plasma TNF- 伪 level in patients with acute gouty arthritis (Spearman rr 0.55U Pn0. 014), and there was a positive correlation between plasma mi R-3146 level and plasma IL-1 尾 level (Spearman rn 0.53P 0. 002) .ROC curve showed that plasma mi R-3146 level had certain diagnostic value for acute gouty arthritis. The area under the curve is 0.725 (95%CI:0.596-0.854). The level of mi R-3146 in plasma of patients with acute gouty arthritis after colchicine treatment was significantly lower than that before treatment (P0. 026). Bioinformatics predicted possible target genes of mi R-3146 include ADAMTS4,ADAMTS19,SLC16A1,CXCR6 and so on. Conclusion the expression of mi R-3146 in plasma of patients with acute gouty arthritis is highly specific, and there is a positive correlation between the expression level of mi R-3146 and the level of TNF- 伪 -IL-1 尾, indicating that mi R-3146 may be involved in the pathogenesis of gout. Plasma mi R-3146 has certain diagnostic value for acute gouty arthritis. Colchicine decreased the expression of mi R-3146 in plasma of patients with acute gouty arthritis.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R589.7

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