茅台酒对糖尿病大鼠血清C-反应蛋白、胎球蛋白A水平及胰岛素抵抗的影响

发布时间：2018-10-12 20:00

【摘要】：目的:研究不同剂量茅台酒对糖尿病大鼠血清胎球蛋白A(Fetuin-A,FA)、C-反应蛋白(C-reactive protein,CRP)水平及胰岛素抵抗的影响,并初步探讨三者之间的关系。方法:选取雄性SD大鼠47只,适应性喂养一周后,随机分为空白对照组7只,普通饲料喂养,造模组40只,以高脂高糖饲料喂养,6周后,禁食12小时,造模组腹腔注射STZ(35mg/kg),空白对照组注腹腔注射同体积的柠檬酸钠缓冲液,3天后禁食12小时,测SD大鼠空腹血糖,大于16.7mmol/L为造模成功。将造模成功的27只大鼠随机分为三组,分别为模型组、小剂量茅台酒组、大剂量茅台酒组。对照组予以1ml/kg.d生理盐水灌胃,模型组予以1ml/kg.d生理盐水灌胃,小剂量茅台酒组予以1ml/kg.d茅台酒灌胃,大剂量茅台酒组予以3ml/kg.d茅台酒灌胃。灌胃6周后,禁食12小时,腹腔腹主静脉取血,测总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、空腹血糖(FPG)、空腹胰岛素(FINS)、CRP、FA水平。结果:1.模型组、小剂量茅台酒组、大剂量茅台酒组FPG水平明显高于对照组(P0.05),小剂量茅台酒组FPG水平低于模型组(P0.05),大剂量茅台酒组FPG水平高于模型组(P0.05)。2.小剂量茅台酒组、大剂量茅台酒组FINS水平低于对照组(P0.05),大剂量茅台酒组FINS水平低于模型组(P0.05),小剂量茅台酒组FINS水平低于模型组,但是无统计学差异(P0.05),大剂量茅台酒组FINS水平低于小剂量茅台酒组(P0.05)。3.模型组、小剂量茅台酒组、大剂量茅台酒组稳态胰岛素抵抗指数(HOMA-IR)明显高于对照组(P0.05),小剂量茅台酒组HOMA-IR低于模型组(P0.05),大剂量茅台酒组HOMA-IR高于模型组(P0.05)。4.模型组、小剂量茅台酒组、大剂量茅台酒组血清CRP、FA水平明显高于对照组(P0.05),小剂量茅台酒组血清FA水平低于模型组(P0.05),小剂量茅台酒组CRP水平低于模型组,但是无统计学差异(P0.05),大剂量茅台酒组FA水平、CRP水平明显高于模型组(P0.05)。5.FA与TG、LDL-C、CRP、FPG、HOMA-IR呈正相关(P0.05);CRP与FA、TG、FPG、HOMA-IR呈正相关,与FINS呈负相关(P0.05)。结论:1.小剂量茅台酒可能降低糖尿病大鼠血清FA水平,减轻胰岛素抵抗;大剂量茅台酒可能增加糖尿病大鼠血清FA、CRP水平,加重胰岛素抵抗。2.茅台酒对胰岛素抵抗的影响可能与FA、CRP水平的变化有关。
[Abstract]:Objective: to study the effects of different doses of Maotai liquor on serum fetal protein A (Fetuin-A,FA), C-reactive protein (C-reactive protein,CRP) and insulin resistance in diabetic rats. Methods: 47 male SD rats were randomly divided into control group (n = 7), normal diet group (n = 40) and model group (n = 40) after one week of adaptive feeding. The rats were fed with high-fat and high-sugar diet. After 6 weeks, the rats were fasted for 12 hours. STZ (35mg/kg) was injected intraperitoneally in the model group, and sodium citrate buffer of the same volume was injected intraperitoneally in the blank control group. Fasting for 12 hours after 3 days. Fasting blood glucose was measured in SD rats, which was larger than 16.7mmol/L. The 27 rats were randomly divided into three groups: model group, low dose Maotai liquor group and high dose Maotai liquor group. The control group was treated with 1ml/kg.d saline, the model group with 1ml/kg.d saline, the low dose Moutai wine group with 1ml/kg.d Moutai wine, and the large dose Moutai wine group with 3ml/kg.d Maotai liquor. After 6 weeks, fasting for 12 hours, blood was taken from abdominal main vein, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), fasting blood glucose (FPG), fasting insulin (FINS), CRP,FA) were measured. The result is 1: 1. The FPG level of model group, low dose Maotai liquor group and high dose Maotai liquor group was significantly higher than that of control group (P0.05), the FPG level of small dose Maotai liquor group was lower than that of model group (P0.05), and the FPG level of large dose Maotai liquor group was higher than that of model group (P0.05). The FINS level of low dose Maotai liquor group and high dose Maotai liquor group was lower than that of control group (P0.05), the FINS level of large dose Maotai liquor group was lower than that of model group (P0.05), and the FINS level of small dose Maotai liquor group was lower than that of model group. However, there was no statistical difference (P0.05), the FINS level of high dose Maotai liquor group was lower than that of small dose Maotai liquor group (P0.05). The steady-state insulin resistance index (HOMA-IR) in model group, low dose Maotai liquor group and high dose Maotai liquor group was significantly higher than that in control group (P0.05). The HOMA-IR of low dose Maotai liquor group was lower than that of model group (P0.05), and the HOMA-IR of high dose Maotai liquor group was higher than that of model group (P0.05). The serum CRP,FA level of model group, small dose Maotai liquor group and large dose Maotai liquor group was significantly higher than that of control group (P0.05), the serum FA level of small dose Maotai liquor group was lower than that of model group (P0.05), and the CRP level of small dose Maotai liquor group was lower than that of model group. However, there was no statistical difference (P0.05). The level of FA and CRP in high dose Maotai liquor group was significantly higher than that in model group (P0.05). 5.FA was positively correlated with TG,LDL-C,CRP,FPG,HOMA-IR (P0.05); CRP and FA,TG,FPG,HOMA-IR, and negatively correlated with FINS (P0.05). Conclusion: 1. Low dose Moutai wine may decrease serum FA level and reduce insulin resistance in diabetic rats, while high dose Maotai wine may increase serum FA,CRP level and increase insulin resistance in diabetic rats. 2. The effect of Maotai wine on insulin resistance may be related to the change of FA,CRP level.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R587.1

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