库欣综合征中FHIT、Ki-67、PCNA的表达及相关因素的研究

发布时间：2018-10-14 21:04

【摘要】：目的探讨库欣综合征肾上腺皮质病变中肿瘤抑制基因脆性组氨酸三联体(FHIT)、肿瘤增殖抗原Ki-67、增殖细胞核抗原(PCNA)的表达,探索鉴别库欣综合征肾上腺皮质病变的相关因素。方法应用免疫组织化学方法检测49例库欣综合征肾上腺皮质病变(腺癌14例、腺瘤26例、增生9例)中FHIT、Ki-67、PCNA的表达,对临床病理及生化指标采用有序结果的累积比数Logistic回归分析探索鉴别库欣综合征皮质病变的相关因素。结果 FHIT在肾上腺皮质增生中表达最高,腺瘤次之,腺癌最低(分别为100%、96.15%和42.96%),Ki-67与PCNA在肾上腺皮质腺癌的表达最高,腺瘤次之,增生最低(分别为85.71%、7.69%、0.00%和100%、96.15%、77.78%)。应用Logistic回归分析发现FHIT、PCNA两个因素与鉴别腺癌、腺瘤、增生的关系更为密切。结论 FHIT、Ki-67和PCNA的表达与库欣综合征肾上腺皮质病变密切相关。Logistic回归分析提示FHIT、PCNA与鉴别诊断的关系更为密切。
[Abstract]:Objective to investigate the expression of tumor suppressor gene fragile histidine triad (FHIT),) tumor proliferating antigen (Ki-67,) proliferating cell nuclear antigen (PCNA) in adrenal cortical lesions of Cushing's syndrome, and to explore the relevant factors in differentiating adrenal cortical lesions of Cushing's syndrome. Methods Immunohistochemical method was used to detect the expression of FHIT,Ki-67,PCNA in 49 cases of adrenal cortical lesions with Cushing's syndrome (14 cases of adenocarcinoma, 26 cases of adenoma and 9 cases of hyperplasia). The clinicopathological and biochemical parameters were analyzed by Logistic regression analysis of cumulative ratio of ordered results to explore the related factors in differentiating the cortical lesions of Cushing's syndrome. Results the expression of FHIT was the highest in adrenocortical hyperplasia, followed by adenoma and adenocarcinoma (96.15% and 42.96%, respectively). The expression of Ki-67 and PCNA was the highest in adrenocortical adenocarcinoma, followed by adenoma, and lowest in hyperplasia (85.71% and 77.78%, respectively). Logistic regression analysis showed that the two factors of FHIT,PCNA were more closely related to the differentiation of adenocarcinoma, adenoma and hyperplasia. Conclusion the expression of FHIT,Ki-67 and PCNA is closely related to the adrenal cortical lesions of Cushing's syndrome, and Logistic regression analysis shows that FHIT,PCNA is more closely related to differential diagnosis.
【作者单位】：广西医科大学第一附属医院内分泌科;广西医科大学公共卫生学院流行病学与统计学教研室;广西医科大学第一附属医院病理科;
【基金】：国家自然科学基金项目(No:81060220) 广西自然科学基金项目(No:2011GXNSFA018172) 广西留学回国人员科学基金(No:桂科回0731019) 广西青年科学基金(No:桂科青0728073) 广西卫生厅自筹经费科研课题项目(No:Z20133027)
【分类号】：R586.2

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