原子荧光分光光度法检测人尿中锡

发布时间：2018-10-17 17:04

【摘要】：目的建立尿中锡的原子荧光分光光度测定方法。方法取2.50 m L尿样加入1.50 m L浓硝酸后,用石墨消解仪消化,以体积分数为4.00%的盐酸溶液定容至10.00 m L,加入质量浓度为100 g/L的硫脲-抗坏血酸2.50 m L,以体积分数为4.00%的盐酸溶液定容至25.00 m L(相当于稀释尿样10倍)后,取1.00 m L样品采用原子荧光光度法进行测定。结果尿中锡在质量浓度为4.00~200.00μg/L范围内线性关系良好,相关系数为0.999 5;方法检出限为0.20μg/L,加标回收率为100.20%~100.84%,批内相对标准偏差(RSD)为0.11%~2.01%,批间RSD为1.37%~5.58%;样品在4℃条件下可保存7 d。结论本方法灵敏度高,准确性好,操作简便,适用于人尿中锡的日常监测。
[Abstract]:Objective to establish an atomic fluorescence spectrophotometric method for the determination of tin in urine. Methods 2.50 mL urine samples were digested by graphite digester after 1.50 mL concentrated nitric acid was added. When the volume fraction of hydrochloric acid was 4.00%, the volume of hydrochloric acid solution was 10.00 mL, the concentration of thiourea-ascorbic acid was 2.50 mL, and the volume fraction of hydrochloric acid solution was 4.00% to 25.00 m L (was 10 times that of dilute urine. 1.00 mL sample was determined by atomic fluorescence spectrophotometry. Results the calibration curve of tin in urine was linear in the range of 4.00 ~ 200.00 渭 g / L, the correlation coefficient was 0.999, the detection limit was 0.20 渭 g / L, the recovery rate was 100.20g / L, the relative standard deviation (RSD) was 0.112.01, the interassay RSD was 1.375.58. the sample could be preserved at 4 鈩，

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