低功率密度微波辐射对小鼠学习记忆的影响

发布时间：2018-10-29 18:07

【摘要】：[目的]观察低功率密度微波辐射对小鼠学习记忆能力的影响。[方法]将40只雄性昆明小鼠随机分成对照组及低、中、高3个微波辐射暴露组(500、1 000、2 000μW/cm~2),每组10只,每天照射1 h,连续照射30 d。照射30 d后进行Y迷宫训练连续7 d,观察小鼠学习记忆能力变化;取海马组织匀浆,用高效液相色谱法观察海马组织中氨基酸含量的变化。[结果]观察学习能力的Y迷宫实验中,与对照组(54.62±3.13)s相比,3个暴露组小鼠的逃避潜伏期[分别(76.24±4.17)s、(95.36±2.31)s、(117.01±4.13)s]均有所延长(P0.05);中、高暴露组小鼠进入错误区域的次数[(7.24±0.57)、(9.86±0.28)次/min]也较对照组[(2.67±0.38)次/min]增加(P0.05);微波辐射后海马组织中谷氨酸的质量分数减少,γ-氨基丁酸增加(P0.05)。[结论]低功率密度微波辐射对小鼠的学习记忆能力有损害,可能是通过γ-氨基丁酸的增加和谷氨酸减少实现的。
[Abstract]:[objective] to observe the effect of low power density microwave radiation on learning and memory ability of mice. [methods] 40 male Kunming mice were randomly divided into two groups: control group and low, medium and high microwave exposure group (5001 000 ~ 2 000 渭 W/cm~2). 10 mice in each group were irradiated for 1 hour per day for 30 days. After 30 days of irradiation, Y maze training was carried out for 7 days to observe the changes of learning and memory ability of mice, and the changes of amino acid content in hippocampus tissue were observed by HPLC. [results] compared with the control group (54.62 卤3.13) s, the escape latency of the three exposed groups [(76.24 卤4.17) s, () 95.36 卤2.31 s] was compared with that of the control group in Y-maze experiment. (117.01 卤4.13) s] were prolonged (P0.05); The frequency of entering the wrong area in the high exposure group [(7.24 卤0.57), (9.86 卤0.28) times / min] was also higher than that in the control group [(2.67 卤0.38) times / min] (P0.05). After microwave irradiation, the content of glutamate in hippocampus decreased and 纬 -aminobutyric acid increased (P0.05). [conclusion] low power density microwave irradiation may impair the learning and memory ability of mice, possibly through the increase of 纬 -aminobutyric acid and the decrease of glutamate.
【作者单位】：吉林医药学院生理学教研室;
【基金】：吉林省大学生创新训练项目(无编号) 吉林省科技厅科技攻关项目(编号:WL201301)
【分类号】：R594.8

【参考文献】