富血小板血浆促糖尿病创面愈合机制的初步研究
发布时间：2018-10-31 14:00
【摘要】：目的探讨富血小板血浆(PRP)促进糖尿病创面愈合与核苷酸结合寡聚化结构域样受体蛋白(NLRP3)炎性反应复合物/IL-1β信号通路的关系。方法共收集25份组织标本,5份取自5例糖尿病截肢患者截下肢体的远端坏死交界区(DMD组),5份取自前述5例糖尿病截肢患者截下肢体的近端创面(DMP组),5份取自5例非糖尿病截肢患者截下肢体的近端创面(NDM组),5份取自5例糖尿病足溃疡保肢治疗患者的溃疡创面使用PRP治疗前行清创时去除的组织(NPRP组),5份取自前述5例糖尿病足溃疡保肢治疗患者的溃疡创面使用PRP治疗后首次行清创时去除的组织(PRP组)。分别采用Western印迹法检测NIRP3蛋白表达,ELISA检测IL-1β蛋白表达,实时荧光定量PCR检测NLRP3基因和IL-1β基因表达。结果 DMD组和DMP组的IL广1β和NLRP3蛋白相对表达量和基因相对表达量均显著高于NDM组(P值均0.05),DMP组的IL-1β和NIRP3蛋白相对表达量和基因表达量均显著高于DMD组(P值均0.05)。NPRP组的IL-1β和NLRP3蛋白相对表达量和基因相对表达量均显著高于PRP组(P值均0.05)。结论 NLRP3炎性反应复合物/IL-1β信号通路上调可阻碍糖尿病创面的愈合。PRP通过抑制NLRP3炎性反应复合物/IL-1β信号通路,加快糖尿病创面愈合进程,是其促进糖尿病创面愈合的机制之一。
[Abstract]:Objective to investigate the relationship between platelet rich plasma (PRP) and nucleotide binding oligodeoxyribonucleotide domain like receptor protein (NLRP3) inflammatory response complex / IL-1 尾 signaling pathway in diabetic wound healing. Methods A total of 25 tissue specimens were collected from the distal necrotic junction region (DMD group) of 5 patients with diabetic amputation (DMD group) and from the proximal wound surface (DMP group) of 5 patients with diabetic amputation (DMP group). Five cases were taken from the proximal wound of limb amputation in 5 cases of non-diabetic amputation (NDM group), and 5 cases were taken from the ulcer wounds of 5 cases of diabetic foot ulcer treated with limb salvage (NPRP group), and 5 cases were treated with PRP before debridement (NPRP group). Five patients with diabetic foot ulcer treated with limb salvage therapy were treated with PRP for the first time after debridement (PRP group). NIRP3 protein expression was detected by Western blot, IL-1 尾 protein was detected by ELISA, NLRP3 gene and IL-1 尾 gene expression were detected by real-time fluorescence quantitative PCR. Results the relative expression of IL 1 尾 and NLRP3 protein in DMD group and DMP group was significantly higher than that in NDM group (P < 0. 05). The relative expression and gene expression of IL-1 尾 and NIRP3 protein in DMP group were significantly higher than those in DMD group (P < 0. 05). The relative expression of IL-1 尾 and NLRP3 protein and gene expression in). NPRP group were significantly higher than those in PRP group (P < 0. 05). Conclusion the upregulation of NLRP3 inflammatory response complex / IL-1 尾 signaling pathway may inhibit the healing of diabetic wounds. PRP can accelerate the healing process of diabetic wounds by inhibiting the NLRP3 inflammatory response complex / IL-1 尾 signaling pathway. It is one of the mechanisms of promoting wound healing of diabetes mellitus.
【作者单位】： 上海交通大学附属第一人民医院骨科;上海交通大学附属第六人民医院骨科;
【分类号】：R587.2
本文编号：2302450
[Abstract]:Objective to investigate the relationship between platelet rich plasma (PRP) and nucleotide binding oligodeoxyribonucleotide domain like receptor protein (NLRP3) inflammatory response complex / IL-1 尾 signaling pathway in diabetic wound healing. Methods A total of 25 tissue specimens were collected from the distal necrotic junction region (DMD group) of 5 patients with diabetic amputation (DMD group) and from the proximal wound surface (DMP group) of 5 patients with diabetic amputation (DMP group). Five cases were taken from the proximal wound of limb amputation in 5 cases of non-diabetic amputation (NDM group), and 5 cases were taken from the ulcer wounds of 5 cases of diabetic foot ulcer treated with limb salvage (NPRP group), and 5 cases were treated with PRP before debridement (NPRP group). Five patients with diabetic foot ulcer treated with limb salvage therapy were treated with PRP for the first time after debridement (PRP group). NIRP3 protein expression was detected by Western blot, IL-1 尾 protein was detected by ELISA, NLRP3 gene and IL-1 尾 gene expression were detected by real-time fluorescence quantitative PCR. Results the relative expression of IL 1 尾 and NLRP3 protein in DMD group and DMP group was significantly higher than that in NDM group (P < 0. 05). The relative expression and gene expression of IL-1 尾 and NIRP3 protein in DMP group were significantly higher than those in DMD group (P < 0. 05). The relative expression of IL-1 尾 and NLRP3 protein and gene expression in). NPRP group were significantly higher than those in PRP group (P < 0. 05). Conclusion the upregulation of NLRP3 inflammatory response complex / IL-1 尾 signaling pathway may inhibit the healing of diabetic wounds. PRP can accelerate the healing process of diabetic wounds by inhibiting the NLRP3 inflammatory response complex / IL-1 尾 signaling pathway. It is one of the mechanisms of promoting wound healing of diabetes mellitus.
【作者单位】： 上海交通大学附属第一人民医院骨科;上海交通大学附属第六人民医院骨科;
【分类号】：R587.2
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1 严姗姗;PRP对RA-FLS细胞迁移和侵袭的影响[D];扬州大学;2015年
2 刘宸;富血小板血浆对糖尿病大鼠创面巨噬细胞浸润变化的影响[D];南京医科大学;2014年
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