不同糖耐量人群血浆25-羟维生素D与葡萄糖转运子4及胆固醇调节元件结合蛋白1C表达关系的研究
发布时间：2018-11-09 20:38
【摘要】：目的探讨不同糖耐量人群血浆25-羟维生素D[25(OH)D]与葡萄糖转运子4(GluT-4)及胆固醇调节元件结合蛋白1C(SREBP-1C)表达的关系。方法随机分为T2DM组、IGR组及正常对照(NGT)组。检测血糖、血脂、HbA1 c等,电化学发光法测定25(OH)D和FIns。Western blot检测GluT-4及SREBP-1C蛋白的表达,RT-PCR检测GluT-4和SREBP-1C mRNA的表达。结果T2DM组、IGR组25(OH)D低于NGT组[(8.77±4.52)vs(18.35±5.90)vs(30.67±5.47)ng/ml,P0.05],T2DM组低于IGR组(P0.05)。T2DM组、IGR组血浆GluT-4 mRNA及蛋白表达低于NGT组[(0.235±0.412)vs(3.386±0.698)vs(6.325±0.959),(1.235±0.092)vs(2.430±0.153)vs(4.843±0.299),P0.05],T2DM组较IGR组降低(P0.05)。T2DM组、IGR组血浆中SREBP-1C mRNA及蛋白表达水平高于NGT组[(7.570±1.233)vs(5.561±0.820)vs(2.880±0.483),(8.340±1.092)vs(5.279±0.798)vs(2.340±0.135),P0.05],T2DM组较IGR组升高(P0.05)。Pearson相关性分析显示,25(OH)D与血浆中GluT-4 mRNA、蛋白表达呈正相关(P0.05),与HbA_1 c、HOMA-IR、FIns、FPG、2 hPG、TG及SREBP-1C mRNA、SREBP-1C蛋白表达呈负相关(P0.05)。血浆中GluT-4表达与SREBP-1C表达呈负相关(P0.05)。结论血浆25(OH)D降低可引发糖耐量异常,甚至导致糖尿病,其机制可能与血浆中GluT-4表达降低及SREBP-1C表达升高有关。
[Abstract]:Objective to investigate the relationship between 25 hydroxyvitamin D [25 (OH) D] and the expression of glucose transporter 4 (GluT-4) and cholesterol regulatory element binding protein 1C (SREBP-1C) in patients with different glucose tolerance. Methods T2DM group, IGR group and normal control (NGT) group were randomly divided into three groups. Blood glucose, blood lipids and HbA1 c were detected. The expression of GluT-4 and SREBP-1C was detected by ECL and FIns.Western blot, and the expression of GluT-4 and SREBP-1C mRNA by RT-PCR. Results in T2DM group, 25 (OH) D in IGR group was lower than that in NGT group [(8.77 卤4.52) vs (18.35 卤5.90) vs (30.67 卤5.47) ng/ml,P0.05], and that in T2DM group was lower than that in IGR group (P0.05). The expression of plasma GluT-4 mRNA and protein in IGR group was lower than that in NGT group [(0.235 卤0.412) vs (3.386 卤0.698) vs (6.325 卤0.959), (1.235 卤0.092) vs (2.430 卤0.153) vs (4.843 卤0.299), P0.05]. The expression of SREBP-1C mRNA and protein in T2DM group and IGR group was higher than that in NGT group [(7.570 卤1.233) vs (5.561 卤0.820) vs (2.880 卤0.483)]. (8.340 卤1.092) vs (5.279 卤0.798) vs (2.340 卤0.135), P0.05. T2DM group was higher than IGR group (P0.05). Pearson correlation analysis, 25 (OH) D and GluT-4 mRNA, in plasma). The protein expression was positively correlated (P0.05), and negatively correlated with the expression of hPG,TG and SREBP-1C mRNA,SREBP-1C protein in HbA_1. There was a negative correlation between GluT-4 expression and SREBP-1C expression in plasma (P0.05). Conclusion the decrease of plasma 25 (OH) D may lead to abnormal glucose tolerance and even lead to diabetes mellitus. The mechanism may be related to the decrease of GluT-4 expression and the increase of SREBP-1C expression in plasma.
【作者单位】： 遵义医学院附属医院内分泌科;
【基金】：国家自然科学基金(81460168) 贵州省科学技术基金(黔科合J字LKZ[2013]19号) 遵义医学院博士启动基金([2012]F-574)
【分类号】：R587.1
本文编号：2321498
[Abstract]:Objective to investigate the relationship between 25 hydroxyvitamin D [25 (OH) D] and the expression of glucose transporter 4 (GluT-4) and cholesterol regulatory element binding protein 1C (SREBP-1C) in patients with different glucose tolerance. Methods T2DM group, IGR group and normal control (NGT) group were randomly divided into three groups. Blood glucose, blood lipids and HbA1 c were detected. The expression of GluT-4 and SREBP-1C was detected by ECL and FIns.Western blot, and the expression of GluT-4 and SREBP-1C mRNA by RT-PCR. Results in T2DM group, 25 (OH) D in IGR group was lower than that in NGT group [(8.77 卤4.52) vs (18.35 卤5.90) vs (30.67 卤5.47) ng/ml,P0.05], and that in T2DM group was lower than that in IGR group (P0.05). The expression of plasma GluT-4 mRNA and protein in IGR group was lower than that in NGT group [(0.235 卤0.412) vs (3.386 卤0.698) vs (6.325 卤0.959), (1.235 卤0.092) vs (2.430 卤0.153) vs (4.843 卤0.299), P0.05]. The expression of SREBP-1C mRNA and protein in T2DM group and IGR group was higher than that in NGT group [(7.570 卤1.233) vs (5.561 卤0.820) vs (2.880 卤0.483)]. (8.340 卤1.092) vs (5.279 卤0.798) vs (2.340 卤0.135), P0.05. T2DM group was higher than IGR group (P0.05). Pearson correlation analysis, 25 (OH) D and GluT-4 mRNA, in plasma). The protein expression was positively correlated (P0.05), and negatively correlated with the expression of hPG,TG and SREBP-1C mRNA,SREBP-1C protein in HbA_1. There was a negative correlation between GluT-4 expression and SREBP-1C expression in plasma (P0.05). Conclusion the decrease of plasma 25 (OH) D may lead to abnormal glucose tolerance and even lead to diabetes mellitus. The mechanism may be related to the decrease of GluT-4 expression and the increase of SREBP-1C expression in plasma.
【作者单位】： 遵义医学院附属医院内分泌科;
【基金】：国家自然科学基金(81460168) 贵州省科学技术基金(黔科合J字LKZ[2013]19号) 遵义医学院博士启动基金([2012]F-574)
【分类号】：R587.1
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