M2型巨噬细胞在糖尿病小鼠同种异体胰岛移植中的抗排斥作用

发布时间：2018-11-17 06:51

【摘要】：目的:探讨腹膜腔来源的M2型巨噬细胞对糖尿病小鼠同种异体胰岛移植的抗排斥作用。方法:体外分离诱导C57BL/6小鼠腹膜腔M2型和M0型细胞,流式细胞术鉴定巨噬细胞表型。链脲佐菌素(streptozotocin,STZ)诱导C57BL/6雄性小鼠的糖尿病模型作为受体,分离BALB/c小鼠胰岛细胞移植于C57BL/6糖尿病小鼠左肾包膜下。将移植后糖尿病小鼠随机分为3组,每组8只。于胰岛移植术后1,3,7 d分别经尾静脉输注PBS(islet+PBS组)、2.5×106个M0型巨噬细胞(islet+M0组),2.5×106个M2型巨噬细胞(islet+M2组)。移植术后取尾静脉血监测受体血糖水平的变化,记录胰岛存活时间,并于移植后10 d每组随机抽取2只小鼠处死取左肾做病理学检查,观察移植物形态结构和胰岛素表达水平。结果:Islet+PBS组、islet+M0组和islet+M2组移植物的中位存活时间分别为6.5(4~10),7.5(4~10)和24(㧐15)d;与islet+PBS组和islet+M0组比较,islet+M2组移植物调节血糖正常水平时间和中位存活时间明显延长(P0.01)。病理学检查结果显示islet+M2组胰岛移植10 d后移植物结构完整,胰岛素染色阳性;而islet+PBS组和islet+M0组胰岛移植物均被排斥,胰岛素染色阴性,局部见大量淋巴细胞浸润。结论:腹膜腔来源的M2型巨噬细胞能减轻受体对胰岛移植物的免疫排斥反应,延长胰岛移植物的存活时间,从而提高受体对血糖的耐受能力。
[Abstract]:Aim: to investigate the anti-rejection effect of M 2 macrophages derived from peritoneal cavity on islet allograft in diabetic mice. Methods: M 2 and M 0 cells were isolated and induced in vitro from C57BL/6 mice, and macrophage phenotype was identified by flow cytometry. Streptozotocin (streptozotocin,STZ) induced diabetic model of C57BL/6 male mice was used as a receptor to isolate islet cells from BALB/c mice transplanted under the left renal capsule of C57BL/6 diabetic mice. The allograft diabetic mice were randomly divided into 3 groups with 8 mice in each group. On the 7th day after islet transplantation, PBS (islet PBS group, 2.5 脳 106 M0 macrophage group and 2.5 脳 106 M2 macrophage group (islet M2 group) were injected via tail vein respectively. After transplantation, the changes of blood glucose level of the recipients were monitored and the survival time of islets was recorded. At 10 days after transplantation, two mice in each group were randomly selected to take the left kidney for pathological examination to observe the morphologic structure of the graft and the level of insulin expression. Results the median survival time of grafts in: Islet PBS group, islet M0 group and islet M2 group was 6.5 (4 / 10), 7.5 (4 / 10) and 24 (? 15) days, respectively. Compared with islet PBS group and islet M0 group, the normal time and median survival time of graft regulating blood glucose in islet M2 group were significantly prolonged (P0.01). Histopathological examination showed that the grafts in islet M2 group were intact and positive for insulin staining 10 days after islet transplantation, while in islet PBS group and islet M0 group, both islet grafts were rejected, insulin staining was negative, and a large number of lymphocytes were infiltrated locally. Conclusion: type M2 macrophages derived from peritoneal cavity can reduce the immune rejection of the receptor to islet grafts, prolong the survival time of islet grafts, and improve the tolerance of the receptors to blood glucose.
【作者单位】：中南大学湘雅三医院细胞移植与基因治疗中心;中南大学湘雅三医院超声科;
【基金】：国家自然科学基金(81471715,30900359) 湖南省科技计划项目(2015SK20664,2016JJ6149)~~
【分类号】：R587.1

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